Bismark report for: filtered_Unlabeled_NoIndex_L003_R1.fastq Option '--directional' specified: alignments to complementary strands will be ignored Bowtie was run against the bisulfite genome of /Volumes/Bay3/Software/bismark_v0.6.4/genomes/ with the specified options: -q -n 1 -l 50 -k 2 --best --chunkmbs 512 Final Alignment report ====================== Sequences analysed in total: 139728554 Number of alignments with a unique best hit from the different alignments: 46463 Mapping efficiency: 0.0% Sequences with no alignments under any condition: 139581084 Sequences did not map uniquely: 91544 Sequences which were discarded because genomic sequence could not be extracted: 1 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 23771 ((converted) top strand) CT/GA: 22692 ((converted) bottom strand) GA/CT: 0 (complementary to (converted) top strand) GA/GA: 0 (complementary to (converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 9463 Final Cytosine Methylation Report ================================= Total number of C's analysed: 327062 Total methylated C's in CpG context: 51012 Total methylated C's in CHG context: 905 Total methylated C's in CHH context: 5502 Total C to T conversions in CpG context: 18901 Total C to T conversions in CHG context: 32500 Total C to T conversions in CHH context: 218242 C methylated in CpG context: 73.0% C methylated in CHG context: 2.7% C methylated in CHH context: 2.5%