##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Y38_control_GTGAAA_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3448820	
Filtered Sequences	165714	
Sequence length	36	
%GC	19	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.994552919549296	33.0	33.0	34.0	30.0	34.0
2	32.58483568292923	34.0	33.0	34.0	31.0	34.0
3	33.112310297435066	34.0	33.0	34.0	31.0	34.0
4	36.245855394018825	37.0	37.0	37.0	35.0	37.0
5	35.767828126721604	37.0	37.0	37.0	35.0	37.0
6	35.745567759407564	37.0	37.0	37.0	35.0	37.0
7	35.458854042832044	37.0	37.0	37.0	35.0	37.0
8	34.99943719881003	37.0	35.0	37.0	32.0	37.0
9	37.26542005671505	39.0	39.0	39.0	35.0	39.0
10	37.13047129163018	39.0	39.0	39.0	35.0	39.0
11	37.32302033739076	39.0	39.0	39.0	35.0	39.0
12	36.92495085275544	39.0	39.0	39.0	33.0	39.0
13	36.86133634112537	39.0	38.0	39.0	32.0	39.0
14	38.41772287333059	41.0	39.0	41.0	34.0	41.0
15	38.6450107573025	41.0	39.0	41.0	34.0	41.0
16	38.805200909296516	41.0	39.0	41.0	36.0	41.0
17	39.170909470485554	41.0	39.0	41.0	37.0	41.0
18	39.26465080810248	41.0	39.0	41.0	37.0	41.0
19	39.42619475646743	41.0	40.0	41.0	37.0	41.0
20	39.579840061238336	41.0	40.0	41.0	38.0	41.0
21	39.70116967542522	41.0	40.0	41.0	38.0	41.0
22	39.60589418989684	41.0	40.0	41.0	38.0	41.0
23	39.49029697113795	41.0	40.0	41.0	38.0	41.0
24	39.42058646145638	41.0	40.0	41.0	37.0	41.0
25	39.406230826775534	41.0	40.0	41.0	38.0	41.0
26	39.150383029557936	41.0	40.0	41.0	37.0	41.0
27	39.19754263777176	41.0	40.0	41.0	37.0	41.0
28	39.265902250624855	41.0	40.0	41.0	38.0	41.0
29	39.298482959389005	41.0	40.0	41.0	37.0	41.0
30	39.2027449968395	41.0	39.0	41.0	37.0	41.0
31	39.08808230061296	41.0	39.0	41.0	37.0	41.0
32	39.03391072888698	41.0	39.0	41.0	37.0	41.0
33	38.91437216207282	41.0	39.0	41.0	37.0	41.0
34	38.96157352369796	41.0	39.0	41.0	37.0	41.0
35	38.75148543559826	40.0	39.0	41.0	36.0	41.0
36	37.91247180194965	40.0	38.0	40.0	35.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	174.0
3	28.0
4	1383.0
5	439.0
6	475.0
7	370.0
8	408.0
9	471.0
10	601.0
11	590.0
12	733.0
13	874.0
14	973.0
15	1093.0
16	1381.0
17	1701.0
18	2121.0
19	2511.0
20	2855.0
21	3441.0
22	4233.0
23	5139.0
24	5863.0
25	7064.0
26	8507.0
27	10597.0
28	13613.0
29	18586.0
30	29048.0
31	46011.0
32	70854.0
33	78612.0
34	79879.0
35	93615.0
36	115014.0
37	228911.0
38	976498.0
39	1634154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.28666194014106	30.353029672926557	48.897716380023105	0.4625920069092788
2	16.01635148911202	36.367031558188266	47.33229117304552	0.2843257796541951
3	15.98959514308563	31.524622952968212	52.04432067743494	0.4414612265112182
4	16.913089114537726	31.779159248670556	47.78112513845315	3.5266264983385622
5	20.445427711507126	31.454091544354302	47.72800552072883	0.3724752234097459
6	20.53281992101646	31.903665601568072	47.18242761292268	0.38108686449278306
7	17.46614784187055	34.651909928613264	47.492765641581755	0.38917658793442395
8	17.40177799943169	34.49788623355234	47.71750917705186	0.3828265899641037
9	20.327184370306366	31.168747571633197	48.068237832070096	0.43583022599033877
10	17.26862040669572	34.62976655445274	47.72153550396228	0.38007753488925033
11	20.037335894304277	31.32196206561792	48.07985172349401	0.5608503165837947
12	16.836164253280835	31.82601585469813	47.78524248873528	3.5525774032857615
13	16.665898286802857	34.85353664954484	47.9367840838085	0.5437809798438015
14	16.72706016143142	31.745366682798014	47.955663046343176	3.5719101094273875
15	16.799137096166223	34.80549869230635	47.869648169518854	0.525716042008571
16	17.01538497225138	31.49538102887364	47.93451093417458	3.55472306470039
17	20.095568919224547	31.365887462958348	48.16035629577652	0.37818732204058203
18	17.0341018383985	31.293687081686272	51.15948454209081	0.5127265378244192
19	16.966270664556898	31.28306481334084	48.249625440634624	3.5010390814676406
20	17.208958426360322	31.3202486647607	51.06682865443833	0.40396425444064926
21	20.117866400681972	31.516199743680446	47.94808079285089	0.4178530627866922
22	16.940054859343196	34.63033733276889	48.04878190221583	0.38082590567208496
23	16.932139108448688	34.66826334804367	47.83949292801596	0.5601046154916755
24	16.93204276482132	31.524326029570727	48.03953008800701	3.504101117600939
25	16.98159327045723	31.517490321976418	50.946896493932805	0.5540199136335524
26	17.077027148886987	31.483354920570854	47.7478958604986	3.6917220700435545
27	17.034453701343647	31.624784441449993	47.81631613301517	3.5244457241911857
28	17.359087619012268	34.5874183385609	47.573240103190365	0.48025393923646553
29	20.286106074520152	31.452965231912422	47.84493994898295	0.41598874458447666
30	17.198980935159593	31.417463983028732	50.79692007195855	0.5866350098531252
31	17.1400516651993	31.555268655556446	47.77163378723078	3.5330458920134693
32	17.054979777443126	34.743271979832826	47.58667628278462	0.615071959939415
33	17.23018092806313	31.582962525233604	47.640465858302115	3.5463906884011536
34	20.21260031332465	31.619432623167526	47.6853960732645	0.4825709902433268
35	17.333477960250026	31.68805714232681	50.477993893186444	0.5004710042367179
36	20.372116543319844	31.725707287031014	47.454862717944614	0.44731345170452896
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	20.749253947050335
2	16.30067726876621
3	16.431056369596845
4	20.439715612876288
5	20.81790293491687
6	20.913906785509244
7	17.855324429804977
8	17.784604589395794
9	20.763014596296706
10	17.64869794158497
11	20.59818621088807
12	20.388741656566594
13	17.20967926664666
14	20.298970270858806
15	17.324853138174795
16	20.57010803695177
17	20.47375624126513
18	17.54682837622292
19	20.46730974602454
20	17.61292268080097
21	20.535719463468666
22	17.320880765015282
23	17.492243723940362
24	20.436143882422257
25	17.53561318409078
26	20.768749218930544
27	20.558899425534833
28	17.839341558248734
29	20.70209481910463
30	17.78561594501272
31	20.67309755721277
32	17.670051737382543
33	20.776571616464285
34	20.695171303567975
35	17.833948964486744
36	20.819429995024375
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8089.0
1	24784.0
2	41479.0
3	41479.0
4	86160.5
5	130842.0
6	130842.0
7	199241.5
8	267641.0
9	267641.0
10	335524.0
11	403407.0
12	403407.0
13	446737.0
14	490067.0
15	494184.5
16	498302.0
17	498302.0
18	469542.0
19	440782.0
20	440782.0
21	394739.5
22	348697.0
23	348697.0
24	300736.5
25	252776.0
26	211150.5
27	169525.0
28	169525.0
29	139027.0
30	108529.0
31	108529.0
32	87497.5
33	66466.0
34	66466.0
35	53312.0
36	40158.0
37	40158.0
38	32211.5
39	24265.0
40	19339.5
41	14414.0
42	14414.0
43	11663.5
44	8913.0
45	8913.0
46	7389.5
47	5866.0
48	5866.0
49	7557.0
50	9248.0
51	19744.0
52	30240.0
53	30240.0
54	55989.5
55	81739.0
56	81739.0
57	43675.0
58	5611.0
59	5611.0
60	3225.0
61	839.0
62	839.0
63	609.0
64	379.0
65	294.0
66	209.0
67	209.0
68	168.0
69	127.0
70	127.0
71	106.0
72	85.0
73	85.0
74	64.5
75	44.0
76	31.5
77	19.0
78	19.0
79	20.0
80	21.0
81	21.0
82	18.5
83	16.0
84	16.0
85	12.5
86	9.0
87	9.0
88	8.0
89	7.0
90	4.0
91	1.0
92	1.0
93	2.0
94	3.0
95	3.0
96	3.0
97	3.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08753718663194948
2	0.08040431219953491
3	0.0013337895280124796
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0014497712261005212
11	0.003711414338817335
12	0.0
13	1.4497712261005212E-4
14	0.001420775801578511
15	0.0
16	0.0
17	0.0
18	8.698627356603129E-5
19	0.002928537876723053
20	0.0
21	0.0
22	0.0
23	0.0
24	1.1598169808804172E-4
25	7.828764620942815E-4
26	6.668947640062398E-4
27	0.006407988819364305
28	0.012642005091596546
29	0.004900226744219762
30	0.004871231319697752
31	0.013453876978212839
32	0.01284497306325062
33	0.0013337895280124796
34	2.899542452201043E-5
35	0.025168028485105052
36	2.3196339617608344E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
36	3448820.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	10.198144210322829
#Duplication Level	Relative count
1	100.0
2	5.990808210184158
3	0.7857150545157089
4	0.2642370491556254
5	0.12700599511349817
6	0.07588070047035271
7	0.05327793862811999
8	0.0430528796994909
9	0.031213337782130902
10++	0.2610080831781636
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTG	77030	2.233517550930463	TruSeq Adapter, Index 1 (97% over 35bp)
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CACAC	109395	4204.779	125131.945	12
ACTCC	107580	2771.5085	81655.3	23
CTCCA	107200	2761.7192	81369.75	24
CCCCC	55	1296.6907	11315.061	16
CACGT	223055	420.2416	6267.3125	14
GCACA	112270	315.58163	9395.4375	11
AGCAC	111900	314.54163	9350.155	10
ACACG	111680	313.9232	9331.364	13
CCCAC	260	247.50446	4264.085	12
TCACG	111865	210.75667	6171.673	30
ACGTC	111600	210.2574	6197.7705	15
GTCAC	111425	209.9277	6162.143	29
CAGTC	110750	208.65599	6132.3345	27
TCCAG	110445	208.08136	6129.345	25
CCAGT	110405	208.00598	6115.912	26
CTCCC	255	162.70052	4593.782	24
CGTCT	111195	140.41432	4151.4507	16
CACCC	140	133.27162	2284.3308	12
AACTC	110645	115.09395	3390.2222	22
ACCCC	85	80.91492	913.76025	23
CCTCC	115	73.37474	1122.9244	24
CCCCA	75	71.395515	1218.453	24
CGCCC	35	60.345394	1379.1788	10
CCACC	45	42.837307	304.57745	12
CACGC	605	42.11797	857.5528	12
CGCAC	525	36.54865	857.5528	12
CCACG	525	36.54865	345.24854	13
CGCGC	210	26.478765	60.52603	29
CGGAA	124350	25.56208	704.4974	4
CTCCG	520	24.263557	701.7593	24
AGAGC	115950	23.835331	697.0917	8
GAGCA	114725	23.583513	697.3577	9
CCGTC	435	20.2974	134.36479	15
CCCAG	280	19.492615	467.83887	26
CCCTC	30	19.141237	408.28293	22
ATCGG	133145	18.344856	478.15436	2
TCGGA	129720	17.872957	476.71786	3
GATCG	127265	17.534704	477.4836	1
GCTCC	375	17.497757	492.68167	23
GCCCC	10	17.24154	275.8318	11
GCACC	240	16.707954	423.2177	11
GCCCA	235	16.359873	334.11926	11
CGCTC	350	16.33124	470.64258	2
ACGTG	118015	16.26023	460.8833	32
GCCAC	225	15.663708	233.88347	11
AGCCC	200	13.923295	278.43674	10
CGGCG	1410	13.001693	22.132683	28
TCGCG	3750	12.79631	61.151318	30
CGTCG	3405	11.61905	56.7739	16
CGCGG	1235	11.388009	23.61169	31
CGCGT	3275	11.175445	55.703175	31
CACCG	155	10.790554	111.37001	14
GTCTG	114475	10.571563	311.2827	17
CCAGC	145	10.094389	267.3365	26
GCGCG	1075	9.912638	22.131271	30
CGCGA	1890	9.622241	15.476657	24
CCCCT	15	9.570619	102.11159	31
ACGCG	1870	9.520418	69.229546	13
CGACG	1735	8.833116	13.850868	32
TGAAC	115955	8.820892	254.464	20
AGTCA	114905	8.741018	254.07971	28
CTGAA	114510	8.71097	255.04341	19
GAACT	114425	8.704503	254.85384	21
ACGCC	125	8.70206	167.06082	23
CCCGC	5	8.62077	275.87378	30
CCCCG	5	8.62077	275.82538	12
CGTCC	175	8.16562	223.94133	16
CCCGT	170	7.932317	104.50461	14
ACCCG	110	7.6578126	155.91869	13
CTCAC	295	7.59988	140.12698	12
CGCCA	105	7.3097305	155.93703	24
ACCAC	180	6.918599	92.23485	13
CACGG	1345	6.8475733	146.6605	31
GGCAC	1310	6.669384	161.27019	10
CCACA	170	6.534232	30.750387	26
CTTCC	360	6.2162185	168.50337	6
GCACG	1185	6.0329924	66.7877	11
GGGGG	121320	5.9829855	8.21058	1
TCCCA	230	5.92533	169.00287	25
TCTGA	114765	5.851559	171.2292	18
ACACC	150	5.765499	79.93686	13
CGGAC	1050	5.3456893	29.32011	4
CAGCC	75	5.2212358	144.81776	27
TCCCG	110	5.1326756	82.12553	30
GCGCC	40	5.0435743	100.85916	9
TCCAC	195	5.0236497	65.95234	25
CACCA	125	4.8045826	92.23484	12
CACTC	175	4.5084033	49.468918	27
GCGTC	1225	4.1801276	45.309937	15
AGCGC	800	4.0729065	62.71619	10
CGGTC	1155	3.9412632	49.14308	27
ACGGC	715	3.6401603	52.12623	15
GTCGC	1045	3.565905	49.14038	29
AACCC	90	3.4592996	49.19128	22
CACAT	3300	3.4326904	90.19409	12
CCCGG	25	3.1522343	40.349987	26
CGGGC	330	3.0429494	7.3769407	25
TCGGC	880	3.0028675	7.0966344	3
GCGCA	585	2.9783127	65.15872	11
ACCTC	115	2.962665	45.33658	23
CCACT	115	2.9626648	78.3201	26
GGAAG	196595	2.9554608	53.641834	5
CCAAC	75	2.8827498	24.60031	26
CGAGC	565	2.8764899	21.990911	8
CGATC	1450	2.731839	19.591825	10
TACAC	2575	2.6785386	69.725685	12
GCGGC	280	2.5818963	7.3760214	23
CATAC	2445	2.5433114	69.8921	12
CCGGC	20	2.5217872	20.176456	27
ACCCA	65	2.4983828	18.44753	23
TTCCA	3570	2.4890177	73.06514	24
ATTCC	3555	2.4785593	73.05878	23
GACCC	35	2.4365766	33.41701	30
ACTCT	3455	2.4088392	68.93179	23
CTCTA	3400	2.3704932	68.26849	24
ACTTC	3365	2.3460906	64.13557	23
GTCCC	50	2.3330343	67.19487	29
TGCCC	50	2.3330343	37.322475	20
CTTCA	3260	2.2728846	63.806503	24
CCCGA	30	2.0884945	55.7052	32
GAAGA	249120	2.0677354	29.716759	6
TCGGG	7890	1.9689419	5.9084454	3
GGCGC	210	1.9364227	7.37723	29
GCCCG	15	1.8913406	20.175346	29
GGCCC	15	1.8913404	20.17625	28
CCGGT	550	1.8767922	46.40955	26
AAGAG	225590	1.8724327	29.491714	7
GCCCT	40	1.8664275	29.858023	21
TCCGG	545	1.8597304	48.04649	25
CGTGA	13300	1.832488	25.51153	32
GAGCC	350	1.7818965	35.837196	9
CGGAG	4745	1.7666603	9.232029	4
CCCAA	45	1.7296498	12.297979	12
CAACC	45	1.7296497	12.298085	19
CACCT	65	1.6745497	8.245955	31
CTGCC	35	1.6331241	37.323555	19
AACGC	580	1.630332	9.443213	22
ACGGG	4245	1.5805001	11.618903	32
CGGGA	4190	1.5600221	6.6113234	4
TCACC	60	1.5457385	12.366316	30
CTCGC	30	1.3998206	14.929422	19
CGCCT	30	1.3998206	29.858847	16
GAGCG	3575	1.3310454	5.777706	9
CGAAC	470	1.321131	8.544044	19
AGATC	17155	1.3050098	6.4522715	32
CTCCT	75	1.2950457	22.10156	24
CCATC	50	1.2881153	20.610554	26
CGGCT	370	1.2625692	32.208267	16
CCGCG	10	1.2608937	20.179237	31
CGGCC	10	1.2608936	20.171423	12
GCCGC	10	1.2608936	20.174963	30
CCAGG	240	1.2218719	31.769764	26
TACGT	23345	1.1902988	5.442698	31
TTACG	23090	1.177297	5.506811	30
AGCTC	620	1.1680967	22.002014	22
ACGTT	22250	1.1344678	6.4276214	15
CAGGC	220	1.1200494	26.884058	27
ACGCT	580	1.0927355	19.321453	1
ATACG	14030	1.0672859	6.0848684	13
CCGAC	15	1.0442472	11.1392145	29
ACCGC	15	1.0442472	11.141362	31
GACAC	370	1.0400393	6.7453775	9
CCTAC	40	1.0304922	8.2427635	12
GACTC	540	1.0173745	23.8104	22
TCTCG	700	0.8839429	5.6574216	30
TCCGA	460	0.8666523	18.988588	8
AGGGC	2160	0.804212	12.508382	8
CCGAT	425	0.8007114	18.98867	9
CTCGT	620	0.78292084	5.0523386	31
TCTCC	45	0.7770273	8.287363	23
AGCCA	250	0.7027292	8.993992	10
CCGCA	10	0.69616485	11.141364	31
CAGCA	230	0.6465109	6.2971277	27
CGCTG	185	0.63128465	13.6475725	16
CGCCG	5	0.63044685	20.171747	8
TTCCG	480	0.60613227	12.727168	7
CTCTC	35	0.60435456	5.525389	24
CACAG	215	0.60434717	6.2955575	12
GGAGC	1515	0.56406534	6.9689555	8
GCTCT	445	0.5619351	12.524877	3
GCCAG	110	0.5600246	10.589691	25
GGGCA	1470	0.547311	12.687128	9
GCTCA	285	0.53694767	10.8507	11
CGGCA	105	0.5345689	7.3300266	4
TACCC	20	0.5152461	16.488419	30
ACCCT	20	0.5152461	12.368933	31
CACGA	180	0.50596505	5.8481383	31
TCGCC	10	0.4666069	7.466097	29
CCGCT	10	0.4666069	14.929294	13
GAACC	155	0.43569216	8.9934845	21
TAGTC	7870	0.40127015	5.3277245	27
CCTGA	210	0.39564562	7.8365035	19
AGTAC	5140	0.3910085	5.2088437	10
CCCTA	15	0.38643458	12.368575	32
ACATG	5075	0.38606384	6.9124107	13
AGTCC	195	0.36738527	7.5368376	28
CCGTG	105	0.3582967	5.460937	32
CGACC	5	0.3480824	11.13747	10
CCTCT	20	0.34534553	5.5249095	23
CCAAG	115	0.32325548	7.1961956	26
GTACA	4175	0.31759933	5.354809	11
GTCCA	165	0.31086442	5.1248183	29
GCATA	3970	0.3020046	5.415659	11
CTGAC	160	0.30144432	6.329484	19
TCCTG	235	0.29675224	5.252444	18
AGCAT	3865	0.29401708	5.3670564	10
GCCTC	5	0.23330344	7.464765	8
CCTGC	5	0.23330344	7.4645057	21
CCTCG	5	0.23330344	7.467321	32
CTAGT	4480	0.2284232	5.1968064	26
TCTAG	4415	0.22510901	5.1966934	25
GTCCT	160	0.20204408	5.050427	17
>>END_MODULE