FastQCFastQC Report
Fri 13 Dec 2013
Y54_Mix_GTGGCC_L005_R2.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename Y54_Mix_GTGGCC_L005_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 15646291
Filtered Sequences 787245
Sequence length 36
%GC 23

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT 388364 2.48214736642697 Illumina Single End PCR Primer 1 (100% over 36bp)
GATCGGAAGAGCGTCGGGTAGGGAAAGAGGGTAGAT 133916 0.855896135384418 Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGAAGAGCGTCGGGTAGGGAAAGAGTGTAGAT 129784 0.8294873206691604 Illumina Single End PCR Primer 1 (97% over 36bp)
GATCGGAAGAGCGTCGTTTAGGGAAAGAGTGTAGAT 60260 0.3851392000826266 Illumina Single End PCR Primer 1 (97% over 36bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGAT 54305 0.3470790617405748 Illumina Single End PCR Primer 1 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 42225 0.2698722655739945 No Hit
GATCGGAAGAGCGTCGGGTAGGGAAAGGGGGTAGAT 29449 0.18821713082033306 Illumina Single End PCR Primer 1 (96% over 27bp)
GATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTAGAT 27591 0.17634211200597 Illumina Single End PCR Primer 1 (97% over 36bp)
GATCGGAAGAGCGTCGTATAGGGAAAGAGTGTAGAT 17352 0.1109016827055051 Illumina Single End PCR Primer 1 (97% over 36bp)

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 653265 2946.7627 15033.129 27
GAGGG 447480 232.65047 5403.342 27
GAGCG 1274960 198.02425 6275.838 9
TAGGG 1451965 134.60364 4262.976 19
GGGGT 160090 128.76276 2159.75 28
AGGGG 238770 124.13952 1491.061 26
TCGGG 513505 123.385 3819.105 14
GTCGG 492245 118.27665 3731.6794 13
CGGGT 488770 117.44167 3713.667 15
GGGCG 84235 113.511505 2351.2546 9
GTAGG 1181270 109.50901 3459.8257 18
GCGTC 1428795 102.56027 3260.1855 11
CGTCG 1317315 94.55812 2988.5042 12
GGGAA 1557400 93.3265 2812.8838 21
GGAAG 1540825 92.33325 2802.3718 5
GAGTG 987695 91.56374 2881.3364 27
AGGGA 1476220 88.46182 2789.1074 20
GGGTA 912720 84.61321 1429.478 16
GGTAG 895290 82.997375 1382.5234 17
GGGGA 156890 81.56908 866.10535 21
GGGTG 90340 72.661804 1198.5425 27
CGGGG 51025 68.75913 1616.0531 15
GGGAG 100850 52.433178 554.63007 17
TGGGG 65140 52.393074 337.39658 19
GGCGG 37430 50.43908 1121.1244 13
GCGGG 35095 47.29254 1088.6838 14
ATCGG 1699085 47.055157 1460.4366 2
TCGGA 1672325 46.31406 1455.0382 3
GGGGC 32945 44.395283 903.05566 11
GATCG 1571145 43.51194 1350.887 1
GGAGG 82465 42.874588 607.6632 18
AGGGT 419555 38.89462 1005.19055 28
AGCGT 1384120 38.332394 1216.3529 10
GTCGT 869105 37.235764 1174.924 13
GGTGG 44335 35.659298 305.2407 12
GTGGG 43315 34.8389 270.41254 18
TGTAG 1909340 31.561256 605.6175 30
GTGTA 1886790 31.188501 609.31213 29
TCGTG 723320 30.989777 978.3364 14
CGTGT 721540 30.913515 973.7383 15
CGGAA 1675940 30.002323 945.2088 4
AGAGC 1419675 25.41472 804.45703 8
AGAGG 384105 23.017324 629.9069 26
AAGAG 2852485 19.701658 318.59097 7
AGTGT 1140620 18.854366 593.12 28
GTAGA 1613145 17.23648 534.12085 31
AAGGG 286665 17.178274 235.51433 25
GGCGT 60350 14.500898 403.15765 10
GGAAA 1819450 12.5666485 344.11993 22
GGTCG 49970 12.006792 339.5858 12
GGGTC 49390 11.867429 356.71054 11
AGAGT 1059020 11.315645 352.35062 26
GAAGA 1473075 10.174293 313.451 6
GAAAG 1459980 10.083849 311.09946 23
AGGGC 60360 9.374995 266.8859 8
GGTGT 60990 8.746918 216.21916 28
GAAGG 130155 7.7994814 153.89655 6
CCCCC 631235 6.7749257 8.16771 26
GCGGC 12320 4.959634 126.47645 11
CGGCG 12195 4.9093127 121.38281 12
TTAGG 288280 4.7652473 149.99155 18
TTGGG 33040 4.738452 68.65962 19
CGGGA 24170 3.7540371 60.687187 4
TAGAT 1748220 3.3307464 104.418495 32
TGTGG 22285 3.196017 50.140945 30
GTGTG 22180 3.1809585 52.80915 29
GGGTT 21595 3.0970602 22.541515 16
CTCTC 797810 3.0504963 3.6578372 31
GAGCT 106070 2.9375465 92.068436 9
GTTGG 19785 2.8374782 47.251873 18
GTGGT 19665 2.8202682 32.550556 31
CCGGG 6980 2.8099222 57.541542 14
GCTTC 216415 2.7699177 86.21493 11
GCCGG 6405 2.5784457 56.59315 13
GCGTG 10085 2.4232242 28.395256 11
CGTGG 10000 2.4028003 31.231821 15
GGTTG 15910 2.2817426 22.23672 17
AAGCG 127040 2.274243 69.06836 9
CGCCG 18910 2.274165 46.063778 12
GGAGA 35275 2.1138387 30.451002 31
TGGGT 13460 1.9303743 31.17994 32
CTTTT 1411545 1.9227523 12.037563 15
ACGGG 12155 1.8878906 35.500786 3
AGCGG 11965 1.8583802 42.145004 10
GATGG 19805 1.836012 30.970554 1
TTTTA 3467330 1.8222449 5.7541757 16
CGTTT 234985 1.7951404 48.274662 15
TCTTT 1308850 1.782865 11.981383 14
TCGTT 231585 1.7691665 48.28011 14
GCGCC 14425 1.7347873 47.23889 11
ATGGG 18515 1.7164233 33.2683 2
TTCTT 1232365 1.6786802 9.390361 13
CGTCT 130770 1.6737386 43.959682 12
GAGAG 27625 1.6554158 15.572217 27
GACGG 10655 1.6549135 33.01064 2
CTTCT 711810 1.6244782 14.284375 12
TGGTG 11290 1.6191623 26.827946 14
GTGGA 17395 1.6125942 13.478577 31
AAGTG 148490 1.5866181 47.886074 27
GGACG 10140 1.5749246 32.663105 1
GGGCC 3680 1.4814489 14.439715 11
TGGGA 15900 1.474001 20.59427 20
CGGGC 3650 1.469372 23.94258 15
CAGGG 9200 1.4289259 32.83786 19
TTTAG 479080 1.4120474 29.868774 17
GCGCG 3500 1.4089868 10.247118 9
GGCCG 3230 1.3002934 14.310665 12
CTTCG 98985 1.2669191 30.416601 12
GGGCA 8025 1.246427 12.580191 21
AAAGG 177880 1.2285886 24.063936 24
AAAGA 1467750 1.1684388 35.06682 24
GCAGG 7360 1.1431407 27.141567 18
GAGGT 11750 1.0892774 16.341848 27
GAGTT 65160 1.07709 31.973417 27
AGCTT 215210 1.062735 33.233784 10
GTTTA 345680 1.0188624 18.52111 16
TGGAG 10675 0.9896202 10.424054 17
TATGG 58840 0.97262096 28.885157 19
TCGCG 13465 0.96653044 9.1239195 14
TAGTG 58325 0.96410805 19.91514 19
CGCGG 2275 0.9158414 5.472603 13
GTCTT 113535 0.86733747 25.143095 13
TATCG 175285 0.8655801 18.55444 1
GGCGC 2140 0.8614948 6.251225 10
CGACG 18350 0.85143095 5.594694 12
CGCGT 11680 0.8384014 8.618629 15
TTCGT 101100 0.7723416 15.7732315 13
GCCGT 10725 0.7698506 17.79414 13
GGCAG 4845 0.7525158 8.981067 17
GGGCT 2985 0.7172358 7.3471127 9
GAGCA 39925 0.71472883 20.769228 9
GGGAC 4595 0.7136863 10.392331 21
GCGGT 2950 0.7088261 10.218906 14
CGGTG 2935 0.7052219 11.716735 15
TGGAA 65575 0.70066994 18.87051 21
AGCGC 14770 0.6853208 18.346992 10
AGGAA 97075 0.6704814 10.251033 21
ATGGA 59515 0.63591874 18.657377 20
CCGTG 8605 0.61767507 14.999953 14
GTGAA 57105 0.61016786 12.947499 21
TCGTA 117765 0.5815389 5.938831 14
GTAAG 54305 0.5802498 11.692607 18
TTCGG 13530 0.5796766 14.389423 13
GAACG 32380 0.5796599 7.866915 9
CGGAG 3695 0.5739001 7.452376 4
ATCGT 115670 0.57119346 5.1749926 2
TCGGT 13330 0.57110786 14.926238 14
CATCG 67140 0.5554757 9.843295 12
TAAGG 51910 0.5546592 15.173367 19
CGTCA 66875 0.5532833 9.743935 12
GGGAT 5905 0.54741985 5.7576756 21
CGGTT 12585 0.5391893 14.432503 15
AGTGG 5735 0.53166014 5.4086323 28
AGTGA 48245 0.5154986 12.902034 20
ATAGG 47965 0.5125068 13.645368 18
GAGGC 3255 0.5055601 5.247785 11
AAAGC 244720 0.5049406 15.088013 8
GAAAA 618235 0.49216136 5.8272395 23
TGAAG 45620 0.48745042 9.771819 5
TAGGA 45005 0.48087916 12.943398 19
AATCG 143400 0.45773682 6.01904 1
TGGGC 1900 0.45653206 6.426928 20
GTAGT 27550 0.45539957 10.630283 18
AAAAG 569640 0.45347604 6.037894 7
GTCGC 6095 0.43750492 9.275914 13
GACCG 9020 0.41852355 5.7952785 1
GCATC 50515 0.4179305 11.597437 11
AAGGA 59860 0.41344342 10.161903 20
AGTTT 139765 0.41194546 11.749363 28
TAGAG 38475 0.411106 10.795578 19
GGTAA 38345 0.4097169 5.3568783 21
TGGTT 15995 0.40902606 6.860291 32
TAGCG 14700 0.4071079 11.1372385 9
TCTTG 53050 0.40526927 11.853837 14
CTTGT 52985 0.4047727 11.730041 15
GTATG 24270 0.40118134 11.440461 18
CGTAT 81105 0.40050703 5.8951893 15
AGAGA 57960 0.4003204 7.2126727 20
CTCGG 5475 0.3930007 5.827728 32
GTTAG 21415 0.35398847 5.544298 17
TTAGA 183365 0.34935093 9.380715 31
GGTTA 21045 0.34787232 5.3970923 16
GCGAC 7415 0.34405237 5.542733 11
GAGAA 49620 0.34271735 7.2338767 21
AAGCT 106015 0.33840287 10.0685005 9
TAGGT 20370 0.33671463 8.024832 19
ATCTG 65065 0.3212994 9.436287 2
TCTGA 64065 0.3163613 9.377927 3
GATCT 63880 0.31544775 6.451212 1
TCTGG 7350 0.31490192 7.5144944 14
CGTGC 4175 0.29968545 7.3447704 15
GTCTG 6900 0.29562223 7.6812124 13
ACCGG 6325 0.29347688 5.8842845 2
CGTGA 10575 0.29286844 7.1109366 15
CCGGA 6305 0.2925489 5.8482165 3
CTGGT 6770 0.29005253 7.322422 15
AAAGT 221240 0.27246666 7.7604146 26
GGAAT 25480 0.27225417 7.0645404 5
GTCAT 50615 0.24994345 5.8347945 13
GGTAT 14795 0.24456027 5.1524367 17
AGGTA 20960 0.22395794 5.3788424 20
CTGAA 66795 0.21321152 6.111513 4
Produced by FastQC (version 0.10.1)