tag:blogger.com,1999:blog-9031065214003247297.post1889282716001324262..comments2016-02-07T13:32:05.041-08:00Comments on Jake Heare Research Central: 6 24 2015 PCR Miner with Friedman lab dataJake Hearenoreply@blogger.comBlogger2125tag:blogger.com,1999:blog-9031065214003247297.post-19141812871356392192015-06-24T16:44:22.251-07:002015-06-24T16:44:22.251-07:00Couple of things:
1. Make sure when you export th...Couple of things:<br /><br />1. Make sure when you export the data from CFX (and/or the Opticon) that you've made sure that "background subtraction" is OFF. <br /><br />2. Taking a very quick glance at the data, I noticed that the "AverageCT_OfReplicateSamples:" calculated for TLR2.1_HC<br /> by PCR Miner is inaccurate (sort of). More than half of the individuals for that group did not produce a CT value at all. It looks like PCR Miner calculated the "AverageCT_OfReplicateSamples:" from just those samples that actually produced a CT value. This means that using that value is not an accurate representation of TLR2.1 expression of that group.<br /><br />This is a long-standing issue that we've grappled with in the past. It's been so long since we've done serious qPCR expression analysis that I can't remember how we dealt with null values for amplification. Before going further with additional analysis, you should definitely discuss with Steven.Sambhttp://www.blogger.com/profile/03618493573679963503noreply@blogger.comtag:blogger.com,1999:blog-9031065214003247297.post-66256847949808910072015-06-24T16:38:40.158-07:002015-06-24T16:38:40.158-07:00Very cool - would be great to see individual data ...Very cool - would be great to see individual data points, violin? plots, something similar to size at spawning of olys?Steven Robertshttp://www.blogger.com/profile/07075064388692389301noreply@blogger.com