tag:blogger.com,1999:blog-9031065214003247297.post3345055883731063592..comments2016-02-07T13:32:05.041-08:00Comments on Jake Heare Research Central: 5 27 2015 New Primer Check (1-12)Jake Hearenoreply@blogger.comBlogger1125tag:blogger.com,1999:blog-9031065214003247297.post-77016705679596187532015-05-28T15:49:24.369-07:002015-05-28T15:49:24.369-07:00Nice work cranking through these!
A couple of qui...Nice work cranking through these!<br /><br />A couple of quick notes:<br /><br />- Please write down the SR IDs of the primers. This will ensure that everyone knows precisely which primers you're using. We've had issues in the past with same/similar primer names for genes and people not knowing which primers were actually used.<br /><br />- Although fun to start thinking about, you can't really "analyze" this data to gain any insight into the implications. This is due to a couple of factors. The first is that you're only looking at a sample size of n=1. Additionally, you still have to normalize the expression to actin (and/or other genes you determine that have stable expression levels across treatments/individuals). Seeing that your actin expression appears to be highly variable, the rough analysis above is probably meaningless at this point. So, no need to spend time comparing expression levels across treatments/populations at this stage of the game.Sambhttp://www.blogger.com/profile/03618493573679963503noreply@blogger.com