You should add the following to your notebook: - L...

2015-05-01T16:28:29.771-07:00

You should add the following to your notebook:
- Link to qPCR data file
- Plate layout with sample names and well locations
- Cycling parameters (cycling program name is insufficient because programs frequently get changed to customize cycling times and then saved under the same program name).
- Clarify if negative control is actually a negative control sample or a no template control.

Not needed for this entry, but just a heads up. After DNasing your RNA, you'll check it again via qPCR to verify that DNasing worked. Remember to include a positive control sample on that qPCR plate. Otherwise, if nothing amplifies there's no way to know if that was due to the success of the DNase treatment or a failure of the qPCR reactions.

Comments on Jake Heare Research Central: 5 1 2015 qPCR Quality Check

You should add the following to your notebook: - L...