Oyster Bay 5-1-14

5-1-14

Shelton WA
Oyster Bay/Totten Inlet
Start Time: 9:30 AM
Finish Time: 5:30 PM
Temps: Mid 60s to Mid 80s

Participants: Jake Heare

I arrived earlier in the morning. I pulled up the hanging devices and broke them down into individual trays. I collected dead, counted live, and photographed trays for size/growth for later analysis. Upon completing these metrics the trays were reorganized into stacks containing one tray from each population at a random level in stack (top, middle, bottom). Since one stack is missing we were unable to completely reorganize the trays as such. One hanging tray has 2 Hood Canal Pops and 1 North Sound pop tray. 2 stacks were then rebuilt and hung off the docks.


The 3rd stack was treated with an epsom salt mixture to anesthetize the animals for brooding larvae inspection. One tray was dosed at a time in a 10 gallon mixture of 50/50 sea water/freshwater with 6-8 cups of epsom salt in them (roughly 75-80 g/L). Gaping oysters in each tray were individually inspected for brooding larvae. Once inspection was completed, trays were then transferred to a tub filled with 10-15 gallons of fresh seawater to recover from treatment for 30 minutes to 1 hour depending on recovery rate. In the first tray we had 25 animals open, in the second we had 3, in the third we had 4. None of these animals showed signs of brooding larvae. 

Temperatures logged at Oyster bay from Feb 28th to May 1st, 2014. Temps logged every 15 minutes. 

Oyster Bay	1N1-4	1N5-8	1N9-12	1N13-16	Total
Live	84	67		100	251
	1H1-4	1H5-8	1H9-12	1H13-16	Total
	89	101	96	14	300
	1S1-4	1S5-8	1S9-12	1S13-16	Total
		74		93	167

Treatment set up at Oyster Bay

Artsy photo of science

Gaping Oyster

Jack trying to help out

Jack decided to drink some epsom salt water behind my back. He made this face when I caught him. 

Manchester 4-30-14

4-30-14

Manchester WA
Clam Bay
Start Time: 10:00 AM
Finish Time: 6:00 PM
Temps: Mid 60s to Mid 80s

Participants: Sammi Brombacher and Jake Heare

We arrived earlier in the morning. We pulled up the hanging devices and broke them down into individual trays. We collected dead, counted live, and photographed trays for size/growth for later analysis. Upon completing these metrics the trays were reorganized into stacks containing one tray from each population at a random level in stack (top, middle, bottom). 3 stacks were then rebuilt and hung off the docks.


The 4th stack was treated with an epsom salt mixture to anesthetize the animals for brooding larvae inspection. One tray was dosed at a time in a 10 gallon mixture of 50/50 sea water/freshwater with 6-8 cups of epsom salt in them (roughly 75-80 g/L). Gaping oysters in each tray were individually inspected for brooding larvae. Once inspection was completed, trays were then transferred to a tub filled with 10-15 gallons of fresh seawater to recover from treatment for 30 minutes to 1 hour depending on recovery rate. In the first tray we had 30 animals open, in the second tray we had 20, in the 3rd tray we had 45. None of these animals had any signs of brooding larvae. 

Temps logged for Manchester from Feb 28th to April 30th, 2014. Temps logged every 15 minutes. 

Manchester	4N1-4	4N5-8	4N9-12	4N13-16	Total
Live	90	97	59	91	337
	4H1-4	4H5-8	4H9-12	4H13-16	Total
	97	85	89	84	355
	4S1-4	4S5-8	4S9-12	4S13-16	Total
	92	91	98	94	375

Tray being treated with epsom salt

Oysters being treated with Epsom Salt

1 tray being treated, 1 tray being examined, 1 tray recovering

Gaping oyster

Sammi processing Trays.

Ferry on the Sound.

For More info see the oly wiki

Dabob Final Sampling 4-18-14

4-18-2014

Dabob WA

Tarboo Creek

Start Time: 11:30 AM

Finish Time: 2:00 PM

Temp: Low to Mid 60's

Participants: Sammi Brombacher and Jake Heare

We reached the site at roughly 10 AM on Friday morning. There was a slight breeze, misty rain, and overall warm temperatures. We walked out to the site at 11:30 when the tide was low enough to reach the trays. We collected all the trays and placed them on a plastic sled and then dragged them back to the truck. This took approximately 2 hours and some swear words. The trays were then driven back to Seattle and cold stored at 12C. Trays stayed here for 14 hours before being processed the following day by Jake Heare.

Logged Temps for Dabob from Jan 30th to April 17th 2014. Temps logged every 15 minutes. 

Dabob	3H1-4	3H5-8	3H9-12	3H13-16	Total
Live	2	16	32	19	69
Dead	1	1	1	0	3
	3N1-4	3N5-8	3N9-12	3N13-16	Total
Live	0	16	0	17	33
Dead	0	7	2	11	20
	3S1-4	3S5-8	3S9-12	3S13-16	Total
Live	14	19	11	15	59
Dead	12	6	8	6	32

For more info see the Oly Wiki

Inaugural Post

This is the first post in what hopes to be a long running record of the scientific work performed by Jake Heare. This should include wet lab, computer science, and field work posts with informational videos and links to supportive information.