6 13 2014. Fidalgo repro check

Anacortes wa

Mid 60s partly cloudy lite rain

Participants:  Sean Bennett and Jake Heare

Used the anesthesia sop with insulation on the treatment group. Temps stayed steady due to insulation and weather. Found multiple brooders in only one population. If you can guess which pop, you get a sticker.

Numbers as follow:

Temps in c

Pretreatment
Initial.         7
45 min.       7
1.5 hrs.       10

Treatment
Initial.          6
45 min.        6
1.5 hrs.         6
2.25 hrs.       6

Recovery
Initial.             7
45 min.           8
1.5 hrs.           9

Salinity in ppt
Pretreatment.     27
Treatment.           66
Recovery.             26

2N1-4
Brood.       0
Gaping.     68
Dead.         1
Closed.       27

2S13-16
Brood.     7
Gaping     83
Dead.       1
Closed.     3

Brooders
#           size.              Sick
1.            30.                W
2.             26.                W
3.             26.                W
4.              25.              W
5.              26.               W
6.              28.               W
7.              25.              W

2H5-8
Brood.         0
Gaping.       72
Dead.           0
Closed.        27

Lots of males with semen. Expect more brooders next week.

6 12 2014 oyster bay repro

Shelton wa

Mid 60s partly cloudy to rainy

Participants:  L. Christine savolainen  and Jake heare

Used anesthesia sop with insulation and semi frozen ice blocks. No change in Temps the entire time. Weather definitely helped. Found several more brooders. Some were below the 20 mm mark.

***Update*** I forgot to mention that I also jumped in the water today during lowtide and hunted underneath the docks to find the lost stack of trays. I was unable to find them. I used a 6 ft pole to feel underneath the lower parts of the dock as well as grab items I found. I pulled out half a dozen shell bags and 1 set of oyster growout cages. Our stacks are long gone. It might be possible to search further under the docks with a diver but I couldn't risk my safety going any further under the docks. At this point in time, its probably safe to assume that we will be unable to ever recover the missing trays. 

Numbers as follow:

Temps
Pretreatment
Initial.  15
45 min. 15
1.5 hrs.   15

Treatment
Initial.       13
45 min.      13
1.5 hrs.      13
2.25 hrs.     13

Recovery
Initial.        13
45 min.       13
1.5 hrs.        13

Salinity.
Pretreatment.        26
Treatment.              64
Recovery.               26

Brood collection
1S13-16
Brood.    3
Gaping.   63
Dead.       11
Closed.     6

Brooders.    
#          size.           Sick
1.            27.            W
2.            26.              W
3.            19.             W

1N5-8
Brood.      2
Gaping      48
Dead.        8
Closed.     1

Brooders
#               size.            Sick
1.                32.               Grey
2.                20.              W

1H1-4
Brood.         1
Gaping.       86
Dead.            8
Closed.         1

Brooders
#         size.          Sick
1.         19.             W

Many males with semen in all treatments.

6 11 2014 Manchester Repro

Manchester WA

Temps Mid 60's to High 70s
Sunny

Participants: Joelle Blais and Jake Heare

Used the Anesthesia SOP to check for brooding larvae. I modified this per Steven and Brent's suggestions to temperature control via adding ice blocks to the solution as well as insulate the tub with 2 inch styrofoam insulation with foil top and bottom. Much to my surprise not only did the insulation work, kept water within 1 degree of ambient with out ice blocks, it actually lowered the temps of the treatment when ice blocks were added (2 degrees below ambient). I believe this insulation and ice blocks method will be the way to go from here on out. Even on the warmest days I see it insulating the tubs very well.

Numbers as follow:

Temps in C

Pretreatment

Initial        9
45 min      15
1.5 hrs      19

Treatment

Initial     11
45 min   11
1.5 hrs    10
2.25 hrs   8

Recovery
Initial       11
45 mins    13
1.5 hrs     15


Salinity in ppt

Pretreatment      25
Treatment          65
Recovery           25


Brood Collection

4H1-4
Brood     0
Gapin      63
Dead       2
Closed     31

4S9-12
Brood       0
Gaping      49
Dead         12
Closed       37

4N9-12
Brood      0
Gaping     41
Dead        8
Closed     12

One male in the 4H group had sperm within its shell. I assume these animals are attempting to spawn though water temps seem to be below expectation.

6 6 2014 fidalgo repro check

Followed the anesthesia sop at fidalgo. Attempted to keep the treatment Temps around 11 c where the ambient water was. The direct sun had other plans. Will need way way way more gel bags. even then it might be more than can actually fit in the tub with the samples.

Also to my and hopefully everyone else's surprise. I found a Brooding oyster! If you can guess which population it came from before you look below, you are one smart cookie.

Numbers as follow

Temps
Pretreatment
Initial.      10
45 min.     15
1.5 hrs.      19

Treatment
Initial.         11
45 min.       16
1.5 hrs.        17
2.25 hrs.      19

Recovery
Initial     10
45 min.   15
1.5 hrs.    19

Salinity
Pretreatment.     20
Treatment.           63
Recovery.             21

Brood collection
2S9-12
Brood.      0
Gaping.    53
Dead.        1
Closed.      43

2H13-16
Brood.       0
Gaping.     58
Dead.         0
Closed.       33

2N5-8
Brood.      1
Gaping.    59
Dead.        2
Closed.      36

Brooders
#         size(mm).        Sick
1.          31.                     W

Lots of nukaluka in the water. Some may have been collected with brooding larvae. May muddy up counting.

Picture of brooders follows.

6 5 2014 oyster bay repro check

Shelton, wa

Participants. Katie Jackson and Jake heare.

High 60s to mid 70s sunny

Followed the anesthesia protocol but modified it by adding ice block and bags of Ice into pools. Temperature started at 13 but stabilized at 16 c. Temp increase unavoidable but non lethal.

Numbers as follow

Temps
Pretreatment.   
Initial. 13
45 min.  16
1.5 hrs.   16

Treatment
Initial.    10
45 min    17
1.5 hrs.   16
2.25 hrs.   16

Recovery
Initial  13
45 min. 17
1.5 hrs 17

Salinity
Pretreatment.     26
Treatment.          63
Recovery.            26

Brood collection.
1S13-16
Brood.  2
Gaping. 60
Dead.     12

Brooders
#     size.    Sick
1.     23.        W
2.     29.        W

1H1-4.   
Brood.   2
Gaping 79
Dead.     7

Brooders
#      size.      Sick
1.       25.         W
2.       24.          Grey

1N5-8
Brood.  1
Gaping.  52
Dead    7

Brooders
#      size.      Sick
1.      30.         W

Multiple males with milt in their shells. Possibly spawning at the moment.

6 4 2014 Manchester Repro Check

Manchester WA

Mid 60s to Low 70s

Followed the Anesthesia SOP to check for brooding oysters. Found no brooders. Water temperatures we also quite low. Last week the surface water temp was around 13 C. This week it was closer to 8 C. I think the bay underwent another turn over event which may lead to either a much later spawn timing for the oysters or an abortive spawn which we will not catch. 

Numbers as Follows:

Temps (Celsius)

Pretreatment 

Initial       8

45 min     9 

1.5 hrs.    10

Treatment

Initial         7

45 min     10

1.5 hr       12

2.25 hr      12

Recovery

Initial      8

45 min    9

1.5 hr     9

Salinity (ppt)

Initial          28

Treatment   63

Recovery    28

Brood Check

4S13-16

Brood    0

Gaping  60

Dead     3

4N1-4

Brood     0

Gaping    43

Dead       15

4H9-12

Brood    0

Gaping   55

Dead      11

Also toured the new hatchery at Manchester with Ryan, saw a chipmunk, and the canadian goose on the docks hatched 4 of it 5 eggs. They goslings were adorable.

Brooding Olys in Hatchery

Flow through with bong screen to catch Oly larvae. 

Various flowthroughs for brooding Olys

Oly larvae (invisible)

Larval Tanks and Ryan's Gesticulation

Chipmunk

Baby Goslings!

Clam Bay with glassy surface waters!

5 30 2014 fidalgo repro check

Anacortes wa

Mid 60s to mid 70s

Performed standard reproduction check using the anesthesia sop. Found no brooders. Also surface water Temps at 3 pm were only 9C.

Temps
Pretreatment
Initial 10
45 min. 12
1.5 hrs   16

Treatment
Initial. 9
45 min.  13
1.5 hrs.  16
2.25 hrs.  19

Recovery
Initial.  9
45 min. 13
1.5 hrs.  16

Salinity
Seawater.  30 ppt
Treatment.  65 ppt

Brood
2S5-8
Brood.   0
Gaping.  77
Dead.      1

2N9-12
Brood.   0
Gaping.  46
Dead.     0

2H1-4
Brood.  0
Gaping. 50
Dead.   1

Also forgot to dessicate 2N tray. Placed it straight in treatment from bath. Still got ok reaction from it.