6 25 2014 Manchester Repro Check

Manchester WA

Mid 60's to mid 70s sunny

Participants: L. Christine Savolainen and Jake Heare

Performed the Anesthesia SOP modified with insulation on the treatment tub as well as ice blocks to keep temperatures within range. Found one brooder in the Northern population with minimal larvae and found one brooder from Dabob that had a very strange mix of eggs and what appeared to be semen. This might be evidence for an abortive spawn. also collected all mortalities from trays.

numbers as follow.

Temps in C

Pretreatment
Initial       10
45 min     15
1.5 hr       15

Treatment
Initial       11
45 min     11
1.5 hrs     11
2.25 hr     11

Recovery
Initial        10
45 min      15
1.5 hr        17


Salinity
Pretreatment      27
Treatment          65
Recovery           21


Brood Collection

4S1-4
Brood    0
Gaping     50
Dead      22
Closed    13

4N5-8
Brood        1
Gaping       53
Dead         23
Closed       19

Brooders
#       size      sick
1        25        w

4H5-8
Brood       1
Gaping      52
Dead        7
Closed      33

Brooders
#        size         sick
1        15           W

Harbor Seal watching us work all day. 

6 20 2014 fidalgo repro check

Anacortes wa

Mid 60s cloudy to sunny

Participants: L. Christine savolainen and Jake heare

Followed the anesthesia sop with insulation on the treatment group.  Found one brooder from the southern population. Two animals looked like possible brooders in the northern pop but they closed quickly and I was unable to confirm brooding in them.

Also Brian found the missing stack at oyster bay. They will be worked up next Thursday as they are safely secured to the dock again.

Numbers as follows

Temps in c
Pretreatment.
Initial.       8
45 min.     8
1.5 hrs.     9

Treatment
Initial.           9
45 min.         9
1.5 hrs.         10
2.25 hrs.       10

Recovery
Initial.        7
45 min.      8
1.5 hrs.      8

Salinity in ppt
Pretreatment.      35
Treatment.           65
Recovery.             35

Brood collection
2S1-4
Brood.       1
Gaping.     83
Dead.         0
Closed.      10
Brooders
#       size.     Sick
1.       27.        W

2H9-12
Brood.        0
Gaping.      77
Dead.          0
Closed.       10

2N13-16
Brood.     0
Gaping.    76
Dead.       0
Closed.    34

6 19 2014. Oyster bay repro

Shelton WA

Mid 60s to high 70s

Participants: L. Christine savolainen and Jake heare

Followed the repro sop with insulation and ice blocks to control temp. Also collected all morts from all trays. Surprisingly, the dabob pop had no brooders while the south sound pop had 11 brooders.

Numbers as follow.

Temps in c
Pretreatment
Initial.            16
45 min.          18
1.5 hrs.          18

Treatment
Initial.            11
45 min.          11
1.5 hrs.          11
2.25 hrs.        10

Recovery
Initial.      16
45 min.     20
1.5 hrs.      16

Salinity
Pretreatment.       25
Treatment.            63
Recovery.              25

Brood collection

1H1-4
Brood.    0
Gaping.  70
Closed.    9
Dead.        7

1N5-8
Brood.     3
Gaping.   54
Dead.       8
Closed.      1

Brooders
#             size.              Sick
1.             29.                  W
2.             26.                  W
3.             30.                  W

1S13-16
Brooders       11
Gaping.          80
Dead.              12
Closed.           1

Brooders
#             size.               Sick
1.             27.                  W
2.             22.                  W
3.             26.                  W
4.             25.                  W
5.             26.                  W
6.             28.                  W
7.             31.                  W
8.             33.                  W
9.             22.                  W
10.           21.                  W
11.           29.                  W

Number 11 could not have its Brood collected because it was attached to the tray.

6 18 2014 Manchester Repro Check

Manchester, WA

Hi 50's to mid 60's, mostly cloudy until later in the day

Participants: L. Christine Savolainen and Jake Heare

Performed the Anesthesia SOP modified with insulators and ice blocks for the treatment tub. Found 1 brooder at Manchester. It seems no matter where they are, South Sound oysters just love to spawn. Also collected all mortalities from all trays checked. Failed to take a picture of the single brooder, I absent mindedly put the tray into recovery before taking the pic and then couldn't find the brooder when I did remember.

Numbers as Follow:

Temps in C

Pretreatment
Initial         7
45 min       8
1.5 hr        10

Treatment
Initial        8
45 min      8
1.5 hr       7
2.25 hr      7

Recovery
Initial         8
45 min       8
1.5 hr        10

Salinity in ppt
Pretreatment     30
Treatment         71
Recovery          30

Brood Collection

4H13-16
Brood      0
Gaping     63
Dead       4
Closed     12

4N13-16
Brood       0
Gaping     73
Dead        2
Closed      12

4S5-8
Brood      1
Gaping     55
Dead       10
Closed     28

Brooder
#           size       sick
1           19         w

Temperature Logger Readings from May to Mid June 2014

Last week I pulled temperature data from the logger pendants out in the field using the Shuttle. Everything performed stellarly and I have the temp graphs on the computer. I'll go by site for each temp graph.

Manchester, WA

From the temperature readings it appears that Manchester has stayed below the 12.5 C mark for most of the logging period. Only recently have temperatures crested the 12.5 mark and stayed that way for several days. It may be soon that the animals will begin spawning and brooding (or spawned within the last week or so). 

Oyster Bay, WA

From this data and the amount of brooders we have seen at Oyster Bay, its clear that temps have been above the 12.5 C mark  since early May. This again correlates with expectations and as temperatures steadily increase I believe we may see a slowing of reproduction due to stress demands. 

Fidalgo Bay, WA

Contrary to the observations in the field, it seems that Fidalgo bay has been much warmer since the beginning of June. Though it does look like in early May there was a warm period for several days. As of June 1st temperatures crested the 12.5 C mark and headed into spawning temps. We saw our first brooder approximately 6 days after that and saw a large number of brooders approximately 12 days after temps went above 12.5. Though temps have somewhat decreased recently I believe we will continue to see spawning across the board.

Overall I think that the populations are responding to temperature cues nicely, though it does seem that North sound respond quickly to temperature increases. The south sound animals seem to have nearly synchronous spawning during warm periods which would be of obvious benefit in South Sound where temps continue to increase and only stay within the spawning window for a short amount of time. The dabob animals seem to have reduced response to temperature cues and do not actively spawn unless temps are somewhat stable for a duration of time. 

6 13 2014. Fidalgo repro check

Anacortes wa

Mid 60s partly cloudy lite rain

Participants:  Sean Bennett and Jake Heare

Used the anesthesia sop with insulation on the treatment group. Temps stayed steady due to insulation and weather. Found multiple brooders in only one population. If you can guess which pop, you get a sticker.

Numbers as follow:

Temps in c

Pretreatment
Initial.         7
45 min.       7
1.5 hrs.       10

Treatment
Initial.          6
45 min.        6
1.5 hrs.         6
2.25 hrs.       6

Recovery
Initial.             7
45 min.           8
1.5 hrs.           9

Salinity in ppt
Pretreatment.     27
Treatment.           66
Recovery.             26

2N1-4
Brood.       0
Gaping.     68
Dead.         1
Closed.       27

2S13-16
Brood.     7
Gaping     83
Dead.       1
Closed.     3

Brooders
#           size.              Sick
1.            30.                W
2.             26.                W
3.             26.                W
4.              25.              W
5.              26.               W
6.              28.               W
7.              25.              W

2H5-8
Brood.         0
Gaping.       72
Dead.           0
Closed.        27

Lots of males with semen. Expect more brooders next week.

6 12 2014 oyster bay repro

Shelton wa

Mid 60s partly cloudy to rainy

Participants:  L. Christine savolainen  and Jake heare

Used anesthesia sop with insulation and semi frozen ice blocks. No change in Temps the entire time. Weather definitely helped. Found several more brooders. Some were below the 20 mm mark.

***Update*** I forgot to mention that I also jumped in the water today during lowtide and hunted underneath the docks to find the lost stack of trays. I was unable to find them. I used a 6 ft pole to feel underneath the lower parts of the dock as well as grab items I found. I pulled out half a dozen shell bags and 1 set of oyster growout cages. Our stacks are long gone. It might be possible to search further under the docks with a diver but I couldn't risk my safety going any further under the docks. At this point in time, its probably safe to assume that we will be unable to ever recover the missing trays. 

Numbers as follow:

Temps
Pretreatment
Initial.  15
45 min. 15
1.5 hrs.   15

Treatment
Initial.       13
45 min.      13
1.5 hrs.      13
2.25 hrs.     13

Recovery
Initial.        13
45 min.       13
1.5 hrs.        13

Salinity.
Pretreatment.        26
Treatment.              64
Recovery.               26

Brood collection
1S13-16
Brood.    3
Gaping.   63
Dead.       11
Closed.     6

Brooders.    
#          size.           Sick
1.            27.            W
2.            26.              W
3.            19.             W

1N5-8
Brood.      2
Gaping      48
Dead.        8
Closed.     1

Brooders
#               size.            Sick
1.                32.               Grey
2.                20.              W

1H1-4
Brood.         1
Gaping.       86
Dead.            8
Closed.         1

Brooders
#         size.          Sick
1.         19.             W

Many males with semen in all treatments.