6 27 2014 fidalgo repro check

Anacortes wa

Mid 50 to mid 60s

Participants:  Sean Bennett and Jake heare

Followed the anesthesia sop modified with insulation on bottom of tub. Too windy to put cover on, also forgot to get ice. Cool enough to keep treatment with 3 degrees. Found brooders in south sound and north sound pop but none in dabob still.

Numbers as follow:

Temps in c

Pretreatment
Initial.         9
45.              10
1.5.             10

Treatment
Initial.            9
45.                 10
1.5.                10
2.25.              12

Recovery
Initial.          10
45.                10
1.5.               14

Salinity in ppt
Pretreatment.           29
Treatment.                58
Recovery.                 28

Brood collection

2S5-8
Brood.          3
Gaping.        87
Dead.            0
Closed.        6

Brooders
#         size.         Sick
1.         27.             W
2.         25.             W
3.         26.             W

2H1-4
Brood.       0
Gaping.     65
Dead.         0
Closed.      28

2N9-12
Brood.      1
Gaping.    87
Dead.        0
Closed.     6

Brooders
#         size.         Sick
1.         31.            W

6 26 2014 oyster bay repro

Used the modified anesthesia sop with insulation and ice blocks. Collected from the water the lost stack as well as the hhn stack to reshuffle oyster trays. Big things of note, 1 south sound tray was 99% dead. It was a middle tray, had no predators and no fouling. No idea why those animals died but the other two trays in the stack survived. Brooding in all sampled trays seemed to have followed expectations. With no pop with way more or way less brooders than expected. Also collected temp logger info. All trays were kept in ambient seawater during Pretreatment or out plant if not sampled

Lost trays marked with orange ziptie, hhn trays labeled with blue ziptie.

Numbers as follows:

Temps in c

Pretreatment
Initial.       14
45.             14
1.5.            14
2.25.          16

Treatment
Initial.        11
45.              11
1.5.             11
2.25.            9
3.                 8

Recovery
Initial.          13
45.                15
1.5.               16
2.25.             16

Salinity
Pretreatment.         26
Treatment.               68
Recovery.                 24

Trays not used for Brood collection

1S9-12
Live.           1
Dead.         82

1H13-16
Live.          12
Dead.         0

Brood collection

1N1-4. From hhn stack
Brood.       2
Gaping.     72
Dead.         10
Closed.      1

Brooders
#        size.      Sick
1.        25.         W
2.        31.         Grey

(Three previous trays were put out immediately as stack 1. Far left from shore)

1H9-12
Brood.       1
Gaping.      92
Dead.         4
Closed.      1

Brooders
#      size.      Sick
1.      27.          Grey

1S1-4
Brood.       11
Gaping.      85
Dead.          10
Closed.       2

Brooders
#          size.         Sick
1.          29.            W
2.          25.            W
3.          28.            W
4.          30.            W
5.          26.            W
6.          30.            W
7.          25.            W
8.          26.            W
9.          28.            W
10.        31.            W
11.        27.            W

10/11. Were dirty samples, had lots of mud mixed in with larvae.

1N9-12
Brood.          5
Gaping.        84
Dead.            16
Closed.          1

Brooders
#        size.        Sick
1.        31.           W
2.        29.           W
3.        36.           W
4.        28.           W
5.        30.           W

6 25 2014 Manchester Repro Check

Manchester WA

Mid 60's to mid 70s sunny

Participants: L. Christine Savolainen and Jake Heare

Performed the Anesthesia SOP modified with insulation on the treatment tub as well as ice blocks to keep temperatures within range. Found one brooder in the Northern population with minimal larvae and found one brooder from Dabob that had a very strange mix of eggs and what appeared to be semen. This might be evidence for an abortive spawn. also collected all mortalities from trays.

numbers as follow.

Temps in C

Pretreatment
Initial       10
45 min     15
1.5 hr       15

Treatment
Initial       11
45 min     11
1.5 hrs     11
2.25 hr     11

Recovery
Initial        10
45 min      15
1.5 hr        17


Salinity
Pretreatment      27
Treatment          65
Recovery           21


Brood Collection

4S1-4
Brood    0
Gaping     50
Dead      22
Closed    13

4N5-8
Brood        1
Gaping       53
Dead         23
Closed       19

Brooders
#       size      sick
1        25        w

4H5-8
Brood       1
Gaping      52
Dead        7
Closed      33

Brooders
#        size         sick
1        15           W

Harbor Seal watching us work all day. 

6 20 2014 fidalgo repro check

Anacortes wa

Mid 60s cloudy to sunny

Participants: L. Christine savolainen and Jake heare

Followed the anesthesia sop with insulation on the treatment group.  Found one brooder from the southern population. Two animals looked like possible brooders in the northern pop but they closed quickly and I was unable to confirm brooding in them.

Also Brian found the missing stack at oyster bay. They will be worked up next Thursday as they are safely secured to the dock again.

Numbers as follows

Temps in c
Pretreatment.
Initial.       8
45 min.     8
1.5 hrs.     9

Treatment
Initial.           9
45 min.         9
1.5 hrs.         10
2.25 hrs.       10

Recovery
Initial.        7
45 min.      8
1.5 hrs.      8

Salinity in ppt
Pretreatment.      35
Treatment.           65
Recovery.             35

Brood collection
2S1-4
Brood.       1
Gaping.     83
Dead.         0
Closed.      10
Brooders
#       size.     Sick
1.       27.        W

2H9-12
Brood.        0
Gaping.      77
Dead.          0
Closed.       10

2N13-16
Brood.     0
Gaping.    76
Dead.       0
Closed.    34

6 19 2014. Oyster bay repro

Shelton WA

Mid 60s to high 70s

Participants: L. Christine savolainen and Jake heare

Followed the repro sop with insulation and ice blocks to control temp. Also collected all morts from all trays. Surprisingly, the dabob pop had no brooders while the south sound pop had 11 brooders.

Numbers as follow.

Temps in c
Pretreatment
Initial.            16
45 min.          18
1.5 hrs.          18

Treatment
Initial.            11
45 min.          11
1.5 hrs.          11
2.25 hrs.        10

Recovery
Initial.      16
45 min.     20
1.5 hrs.      16

Salinity
Pretreatment.       25
Treatment.            63
Recovery.              25

Brood collection

1H1-4
Brood.    0
Gaping.  70
Closed.    9
Dead.        7

1N5-8
Brood.     3
Gaping.   54
Dead.       8
Closed.      1

Brooders
#             size.              Sick
1.             29.                  W
2.             26.                  W
3.             30.                  W

1S13-16
Brooders       11
Gaping.          80
Dead.              12
Closed.           1

Brooders
#             size.               Sick
1.             27.                  W
2.             22.                  W
3.             26.                  W
4.             25.                  W
5.             26.                  W
6.             28.                  W
7.             31.                  W
8.             33.                  W
9.             22.                  W
10.           21.                  W
11.           29.                  W

Number 11 could not have its Brood collected because it was attached to the tray.

6 18 2014 Manchester Repro Check

Manchester, WA

Hi 50's to mid 60's, mostly cloudy until later in the day

Participants: L. Christine Savolainen and Jake Heare

Performed the Anesthesia SOP modified with insulators and ice blocks for the treatment tub. Found 1 brooder at Manchester. It seems no matter where they are, South Sound oysters just love to spawn. Also collected all mortalities from all trays checked. Failed to take a picture of the single brooder, I absent mindedly put the tray into recovery before taking the pic and then couldn't find the brooder when I did remember.

Numbers as Follow:

Temps in C

Pretreatment
Initial         7
45 min       8
1.5 hr        10

Treatment
Initial        8
45 min      8
1.5 hr       7
2.25 hr      7

Recovery
Initial         8
45 min       8
1.5 hr        10

Salinity in ppt
Pretreatment     30
Treatment         71
Recovery          30

Brood Collection

4H13-16
Brood      0
Gaping     63
Dead       4
Closed     12

4N13-16
Brood       0
Gaping     73
Dead        2
Closed      12

4S5-8
Brood      1
Gaping     55
Dead       10
Closed     28

Brooder
#           size       sick
1           19         w

Temperature Logger Readings from May to Mid June 2014

Last week I pulled temperature data from the logger pendants out in the field using the Shuttle. Everything performed stellarly and I have the temp graphs on the computer. I'll go by site for each temp graph.

Manchester, WA

From the temperature readings it appears that Manchester has stayed below the 12.5 C mark for most of the logging period. Only recently have temperatures crested the 12.5 mark and stayed that way for several days. It may be soon that the animals will begin spawning and brooding (or spawned within the last week or so). 

Oyster Bay, WA

From this data and the amount of brooders we have seen at Oyster Bay, its clear that temps have been above the 12.5 C mark  since early May. This again correlates with expectations and as temperatures steadily increase I believe we may see a slowing of reproduction due to stress demands. 

Fidalgo Bay, WA

Contrary to the observations in the field, it seems that Fidalgo bay has been much warmer since the beginning of June. Though it does look like in early May there was a warm period for several days. As of June 1st temperatures crested the 12.5 C mark and headed into spawning temps. We saw our first brooder approximately 6 days after that and saw a large number of brooders approximately 12 days after temps went above 12.5. Though temps have somewhat decreased recently I believe we will continue to see spawning across the board.

Overall I think that the populations are responding to temperature cues nicely, though it does seem that North sound respond quickly to temperature increases. The south sound animals seem to have nearly synchronous spawning during warm periods which would be of obvious benefit in South Sound where temps continue to increase and only stay within the spawning window for a short amount of time. The dabob animals seem to have reduced response to temperature cues and do not actively spawn unless temps are somewhat stable for a duration of time.