7 16 2014 Manchester Repro Check

Manchester WA

Low 70s to Mid 80s

Participants: Alicia Godersky and Jake Heare

Performed the anesthesia SOP with insulation and ice blocks on the treatment group and tarp over the recovery and pretreatment tubs. Finally found multiple brooders in one population. If you can guess the pop, you can consider yourself an Oly Whisperer. The brooding animals were also much smaller than expected. Half were less than 20 mm which is quite odd.  Also temps fluxed on the tubs on the dock even with replacing water, the combination of sun and no breeze just baked the docks.

Numbers as follow:

Temps in C
Pretreatment
Initial     10
45         12
1.5        13

Treatment
Initial      12
45          13
1.5         12
2.25       11

Recovery
Initial      12
45          14
1.5         15


Salinity in ppt
Pretreatment      26
Treatment          66
Recovery           25


Brood Collection
4S5-8
Brood    3
Gaping   59
Dead      12
Closed   12

Brooders
#     size     sick
1      22      w
2      16      w
3      20      w

4H13-16
Brood     1
Gaping    56
Dead       8
Closed    17

Brooders
#     size    sick
1      17     W

4N13-16
Brood     0
Gaping    61
Dead       6
Closed    8

7 11 2014 fidalgo repro check

Anacortes, wa

Low 70s to mid 80s

Performed the anesthesia sop with insulation and ice blocks on treatment and tarp cover on Pretreatment and recovery.  Found lots of brooders in southern pop and none in the other two pops. South sound oysters seem to be very attune to tidal changes. Also temps remained within range using the tarp cover. I think I should add a board or stand to keep the tarp higher over the tubs to keep it out of the water and allow for air flow to keep air temps lower. Also note to self, next week I absolutely have to refill my EtOH, 50 ml falcon tubes, and zipties. Also need a new water proof write in the rain pen as I have lost my only good one.

Numbers as follow :

Temps in c
Pretreatment
Initial.   16
45.         16
1.5.        17

Treatment
Initial.          14
45.                15
1.5.               14
2.25.             14

Recovery
Initial.         16
45.               16
1.5.              17

Salinity
Pretreatment.         20
Treatment.              68
Recovery.               23

Brood collection

2H5-8
Brood.     0
Gaping.   70
Dead.       0
Closed.    28

2S13-16
Brood.       11
Gaping.     81
Dead.         0
Closed.      11

Brooders
#    size.    Sick
1.    27.       W
2.    25.       W
3.    26.       W
4.    30.       W
5.    28.       W
6.    30.       W
7.    27.       W
8.    31.       W
9.    25.       W
10.  32.       W
11.  31.       W

2N1-4
Brood.      0
Gaping.    78
Dead.        0
Closed.     17

7 10 2014 oyster bay repro check

Klamiche shores, wa

Hi 60s to low 80s

Performed the anesthesia sop with insulation and ice blocks on treat. I also covered the Pretreatment and recovery tubs with a tarp to shade them from direct sun to help moderate the water temps. It worked well along with adding fresh seawater and leftover ice blocks, keeping the trays within 3 degrees of ambient. Also I broke the one day record for number of brooders. All pops had multiple brooders with the northern pop almost being equal to the southern pop in terms of numbers. Dabob pop also performed very well. I think this has to do with last week's neap tide and the much warmer than average air temps.

Numbers as follow.

Temps in c
Pretreatment
Initial.        16
45.              16
1.5.             16

Treatment
Initial.         14
45.               14
1.5.              13
2.25.            12

Recovery
Initial.          16
45.               17
1.5.               17

Salinity in ppt
Pretreatment.     25
Treatment.          65
Recovery.            24

Brood collection

1N9-12
Brood.      8
Gaping.    73
Dead.        3
Closed.     2

Brooders
#     size.     Sick
1.     30.        W
2.     22.        W  no sample, very few larvae
3.     39.        W
4.     35.        W
5.     31.        W
6.     33.        W
7.     28.        W
8.     32.        W  no sample tile oyster

1H9-12
Brood.       6
Gaping.     83
Dead.         3
Closed.      3

Brooders
#      size.     Sick
1.      27.        W
2.      27.        W.
3.      28.        W
4.      26.        W
5.      26.        W
6.      27.        W

1S1-4
Brood.      10
Gaping.    82
Dead.        0
Closed.     1

Brooders
#        size.       Sick
1.        22.          W
2.        22.          W
3.        25.          W
4.        28.          W
5.        27.          W
6.        27.          W
7.        25.          W
8.        24.          W
9.        30.          W
10.      30.          W.  No sample, tile oyster

7 9 2014 Manchester Repro Check

Manchester, WA

Hi 60s to Mid 80s, sunny

Participants: Joe Stevick and Jake Heare

Used the anesthesia SOP with insulation modification and ice blocks in both the treatment and recovery trays. Treatment stayed within 1 degree, while recovery trays stayed within 5 degrees. Found 1 brooder from the Dabob population which was pretty interesting considering that none of the other populations have been spawning.

Numbers as follow:

Temps in C

Pretreatment
Initial        11
45 min      13
1.5 hr       16


Treatment
Initial       11
45 min     12
1.5 hr      11
2.25 hr    10

Recovery
Initial       13
45 min     16
1.5 hr      17

Salinity in PPT

Pretreatment     26
Treatment        53
Recovery         27


Brood Collection
4N9-12
Brood       0
Gaping      45
Dead        12
Closed      2

4S9-12
Brood       0
Gaping      55
Dead        22
Closed      15

4H1-4
Brood       1
Gaping      60
Dead         5
Closed      21

Brooders
#       size     sick
1       21       w

7 7 2014 Running Tally of Brooders

Here's an updated running tally of brooders. It appears the numbers for fidalgo were a bit weaker than I thought this morning during lab meeting.

Total Brooders	Manchester	Oyster Bay	Fidalgo
N	1	15	3
H	1	8	1
S	1	41	17
1st brood	6/18/2014	5/29/2014	6/6/2014

7 4 2014 Fidalgo repro check

Anacortes wa

Mid 60s to low 70s

Participants: Jim cerkovnik and Jake heare

Performed anesthesia sop with insulation on treatment tub. Found brood Dr s in all three pops. Majority in southern pop, equal numbers in northern and dabob.

Numbers as follow:

Temps in c
Pretreatment
Initial.        9
45              11
1.5.            12

Treatment
Initial.        12
45.              12
1.5.             12
2.25.           12

Recovery
Initial.          13
45.                13
1.5.               15

Salinity in ppt
Pretreatment.       31
Treatment.            65
Recovery.              32

Brood collection
2H13-16
Brood.     1
Gaping.   88
Dead.       0
Closed.    9

Brooders
#         size.     Sick
1.         23.         W

2S9-12
Brood.      6
Gaping     71
Dead.        0
Closed.     14

Brooders
#     size.     Sick
1.     30.        W
2.     32.         W
3.     32         W
4.      41.        W
5.       32.      Grey
6.       21.       W

2N5-8
Brood.      1
Gaping.     82
Dead.        0
Closed.      13

Brooders
#     size.     Sick
1.       28.      W
 

7 3 2014 oyster bay repro check

Shelton, wa

Mid 60s to high 70s cloudy mostly till 2 pm then sunny

Performed the anesthesia sop with insulation on treatment. No ice blocks needed. Found no brooders in North pop but 3 in dabob. Lots still from south sound pop.

Numbers as follow

Temps in c
Pretreatment
Initial.      15
45.            15
1.5.           16

Treatment
Initial.       11
45.             10
1.5.            11
2.25.          11

Recovery
Initial.     16
45.           19
1.5.           22 sun came out. Kept adding water.

Salinity in ppt
Pretreatment.       25
Treatment.            73
Recovery.              26

Brood collection
1N5-8
Brood.    0
Gaping.   49
Dead.      0
Closed.    1

1H1-4
Brood.       3
Gaping.     66
Dead.         4
Closed.      4

Brooders
#          size.      Sick
1.          25.         W
2.          26.          Grey
3.          25.          W

1S13-16
Brood.     9
Gaping.   78
Dead.      0
Closed.    3

Brooders
#       size.        Sick
1.       26.           W
2.       28.           W
3.       26.           W
4.       30.           W
5.       39.           W
6.       25.           W
7.       28.           W
8.       29.           W
9.       25.           W. No larvae collected from 9. Tile oyster.