7 24 2014. Oyster bay repro check

Oyster bay wa

High 60s to low 70s partly cloudy

Performed the anesthesia sop with insulation on treatment tub and a tarp on the Pretreatment and recovery tubs. Brooding back to normal with most brooders in southern pop, and equal numbers between north and dabob. Also crab fresh cleaning salt water lines used their water for Pretreatment but not for recovery because pump was shut down.

Numbers as follow
Temps in c

Pretreatment
Initial.    15
45.        15
1.5.        15

Treatment
Initial.    12
45.        12
1.5.        12
2.25.     13

Recovery
Initial.    13
45.        13
1.5.        14

Brood collection

1S1-4
Brood.      8
Gaping.     75
Dead.        4
Closed.     7

Brooders
#    size.    Sick
1.      24.      W
2.     34.       W
3.     30.       W
4.     30.      W
5.     30.      W
6.     25.      W
7.     26.      W
8.     27.       W

1N9-12
Brood.       3
Gaping.     68
Dead.        0
Closed.      10

Brooders
#     size.     Sick
1.      33.        W
2.     28.        W
3.     30.        W

1H9-12
Brood.     4
Gaping.    72
Dead.       4
Closed.    6

Brooders
#      size.      Sick
1.        25.        W
2.       26.        W
3.       28.        W
4.       25.        W.    No sample.

Also pics are of whole tray. Brooders between tube and calipers or bag. Closed same time outside of brooders. Rest are gapers.

7 23 14 Manchester Repro Survey

Manchester WA

Hi 50's to mid 60's Rainy, overcast

Participants: Joseph Stevick and Jake Heare

Performed the anesthesia sop with insulation on the treatment tub and tarp on the pretreatment/recovery tubs. No ice blocks because of the cloudy cool conditions. Found a few brooders in the Southern pop and one in the Dabob pop. Very middle of the road spawning.

Numbers as follow

Temp in C

Pretreatment
initial          8
45 min       9
1.5 hr        8

Treatment
Initial         8
45 min       9
1.5 hr        8
2.25  hr     8

Recovery
Initial        8
45 min      8
1.5 hr       8

Salinity in ppt

Pretreatment    27
Treatment        70
Recovery         28

Brood collection

4H5-8
Brood   1
Gaping  67
dead      7
closed    9

brooders
#     size   sick
1     15     w

4S1-4
Brood   2
Gaping  59
Dead     1
closed    6

Brooders
#    size   sick
1    27     w
2    23     w

4N5-8
Brood    0
Gaping   60
Dead     10
Closed   9

Also took pictures of the entire population. In the pics, the tile with the caliper and label tube are the closed animals except for the animals that are between the tube and calipers which were brooders.

7 18 2014 fidalgo bay repro check

Anacortes, WA

Mid 60s to mid 70s

Participants: alicia Godersky and Jake heare

Performed anesthesia sop with insulation on treatment tub.  Found brooders in only the dabob pop. It seems that they spawn later in the season but in smaller number. We will see in the coming weeks. Also treatment was surprisingly ineffective with no discernable reason. Water was calm. Temps were stable. No movement while in tubs. Full treatment strength and time.  Hopefully it was a fluke.

Numbers as follow:

Temps in c
Pretreatment
Initial.     10
45.         11
1.5.         11

Treatment
Initial.   10
45.       10
1.5.       11
2.25.     11

Recovery
Initial.      10
45.          11
1.5.          12

Salinity in ppt
Pretreatment.       28
Treatment.           66
Recovery.            28

Brood collection

2S1-4
Brood.      0
Gaping.     67
Dead.        0
Closed.     30

2H9-12
Brood.      2
Gaping.    32
Dead.       0
Closed.     50

Brooders
#     size.     Sick
1.       19.        W
2.      20.       W

2N13-16
Brood.     0
Gaping.    68
Dead.       0
Closed.    40

7 17 2014 oyster bay repro

Oyster bay

High 60s to high 70s

Participants:  alicia Godersky and Jake heare

Performed anesthesia sop with insulation on treatment and tarp with ice blocks on recovery tubs. Very heavy mussel set on all trays and oysters. Possibly causing spawning issues. Only one population had brooders which is very different than in previous weeks. The one pop was the dabob pop with 5 brooders, one of which looked like aborted spawn.

Numbers as follow.

Temps in c
Pretreatment
Initial.   16
45.      18
1.5.       18

Treatment
Initial.     13
45.         14
1.5.         14
2.25.       14

Recovery
Initial.      16
45.          16
1.5.          17

Salinity in ppt
Pretreatment.       24
Treatment.           66
Recovery.            25

Brood collection
1S13-16.        
Brood.     0
Gaping.     75
Dead.       1
Closed.     2

1N5-8
Brood.      0
Gaping.     48
Dead.        1
Closed.      1

1H1-4
Brood.      5. 
Gaping.     68
Dead.        6
Closed.      1

Brooders
#     size.    Sick
1.       30.      W
2.       20.     W
3.       26.      W
4.       26.      W
5.       27.      W. Possibly abort spawn.

7 16 2014 Manchester Repro Check

Manchester WA

Low 70s to Mid 80s

Participants: Alicia Godersky and Jake Heare

Performed the anesthesia SOP with insulation and ice blocks on the treatment group and tarp over the recovery and pretreatment tubs. Finally found multiple brooders in one population. If you can guess the pop, you can consider yourself an Oly Whisperer. The brooding animals were also much smaller than expected. Half were less than 20 mm which is quite odd.  Also temps fluxed on the tubs on the dock even with replacing water, the combination of sun and no breeze just baked the docks.

Numbers as follow:

Temps in C
Pretreatment
Initial     10
45         12
1.5        13

Treatment
Initial      12
45          13
1.5         12
2.25       11

Recovery
Initial      12
45          14
1.5         15


Salinity in ppt
Pretreatment      26
Treatment          66
Recovery           25


Brood Collection
4S5-8
Brood    3
Gaping   59
Dead      12
Closed   12

Brooders
#     size     sick
1      22      w
2      16      w
3      20      w

4H13-16
Brood     1
Gaping    56
Dead       8
Closed    17

Brooders
#     size    sick
1      17     W

4N13-16
Brood     0
Gaping    61
Dead       6
Closed    8

7 11 2014 fidalgo repro check

Anacortes, wa

Low 70s to mid 80s

Performed the anesthesia sop with insulation and ice blocks on treatment and tarp cover on Pretreatment and recovery.  Found lots of brooders in southern pop and none in the other two pops. South sound oysters seem to be very attune to tidal changes. Also temps remained within range using the tarp cover. I think I should add a board or stand to keep the tarp higher over the tubs to keep it out of the water and allow for air flow to keep air temps lower. Also note to self, next week I absolutely have to refill my EtOH, 50 ml falcon tubes, and zipties. Also need a new water proof write in the rain pen as I have lost my only good one.

Numbers as follow :

Temps in c
Pretreatment
Initial.   16
45.         16
1.5.        17

Treatment
Initial.          14
45.                15
1.5.               14
2.25.             14

Recovery
Initial.         16
45.               16
1.5.              17

Salinity
Pretreatment.         20
Treatment.              68
Recovery.               23

Brood collection

2H5-8
Brood.     0
Gaping.   70
Dead.       0
Closed.    28

2S13-16
Brood.       11
Gaping.     81
Dead.         0
Closed.      11

Brooders
#    size.    Sick
1.    27.       W
2.    25.       W
3.    26.       W
4.    30.       W
5.    28.       W
6.    30.       W
7.    27.       W
8.    31.       W
9.    25.       W
10.  32.       W
11.  31.       W

2N1-4
Brood.      0
Gaping.    78
Dead.        0
Closed.     17

7 10 2014 oyster bay repro check

Klamiche shores, wa

Hi 60s to low 80s

Performed the anesthesia sop with insulation and ice blocks on treat. I also covered the Pretreatment and recovery tubs with a tarp to shade them from direct sun to help moderate the water temps. It worked well along with adding fresh seawater and leftover ice blocks, keeping the trays within 3 degrees of ambient. Also I broke the one day record for number of brooders. All pops had multiple brooders with the northern pop almost being equal to the southern pop in terms of numbers. Dabob pop also performed very well. I think this has to do with last week's neap tide and the much warmer than average air temps.

Numbers as follow.

Temps in c
Pretreatment
Initial.        16
45.              16
1.5.             16

Treatment
Initial.         14
45.               14
1.5.              13
2.25.            12

Recovery
Initial.          16
45.               17
1.5.               17

Salinity in ppt
Pretreatment.     25
Treatment.          65
Recovery.            24

Brood collection

1N9-12
Brood.      8
Gaping.    73
Dead.        3
Closed.     2

Brooders
#     size.     Sick
1.     30.        W
2.     22.        W  no sample, very few larvae
3.     39.        W
4.     35.        W
5.     31.        W
6.     33.        W
7.     28.        W
8.     32.        W  no sample tile oyster

1H9-12
Brood.       6
Gaping.     83
Dead.         3
Closed.      3

Brooders
#      size.     Sick
1.      27.        W
2.      27.        W.
3.      28.        W
4.      26.        W
5.      26.        W
6.      27.        W

1S1-4
Brood.      10
Gaping.    82
Dead.        0
Closed.     1

Brooders
#        size.       Sick
1.        22.          W
2.        22.          W
3.        25.          W
4.        28.          W
5.        27.          W
6.        27.          W
7.        25.          W
8.        24.          W
9.        30.          W
10.      30.          W.  No sample, tile oyster