8 6 2014 Manchester Repro check

Manchester WA

Hi 60s to Low 80s

Performed the anesthesia SOP with insulation and ice blocks on the treatment tub and tarp on the pretreatment and recovery tubs. Forgot my work box when I was packing yesterday and had to borrow a digital thermometer, ruler, refractometer, and notebook from Doug and Andy at the NOAA facility. The thermometer read in Fahrenheit and the refractometer hadn't been calibrated recently. The readings from both seemed accurate enough though the Temp readings will need to be converted to Celsius at some point. As for spawning, the animals at Manchester seem to be increasing their spawning efforts as the next tidal cycle approaches. I had 4 brooders in the southern pop and 2 in the dabob pop, all of pretty good quality. It wouldn't surprise me with the temperatures that if the current temperatures remain through the middle of September these animals might continue spawning until they are forced to stop by temp influence.

Numbers as Follow:

Temps in F
Pretreatment
Initial    64
45        66
1.5       67

Treatment
Initial    68
45        69
1.5       70
2.25     69

Recovery
Initial   61
45       63
1.5      64

Salinity in ppt
Pretreatment      33
Treatment          65
Recovery           34

Brood Collection

4H1-4
Brood   2
Gaping  77
Dead     12
Closed  5

Brooders
#    Size    Sick
1     24      W
2     24      W

4N9-12
Brood     0
Gaping    45
Dead       4
Closed    5

4S9-12
Brood     4
Gaping    50
Dead       11
Closed    7

Brooders
#    size    sick
1    24      W
2    22      W
3    25      W
4    25      Grey

Pics are of all animals. Brooders are between purple ruler and tube label. Closed are on the same tile as brooders. Gaping animals are on all other tiles. Some crossover due to adhered animals on tiles.

8 1 2014 Fidalgo Repro Check

Anacortes WA

Mid 60s to high 70s. Sunny until 2 pm then partly cloudy

Performed the anesthesia SOP with insulation on the treatment tub and ice blocks in the Treatment and recovery tubs to stabilize temperature fluctuations. Had a really poor treatment of the north sound tray and a less than great treatment of the south pop tray. Less than a third of the animals were gaping in north pop and only a little over half in the South pop were open. The Dabob tray was over 95% treated. I'm not sure we missed any brooders though as South sound only had a few and Dabob had none. I'm not sure why the animals didn't open but I assume that it might be due to the fact that they were quickly sprayed with fresh water to help knock off some of the more intense sediment deposits on them. This great to know for next time so I don't accidentally deter treatment. It seems like spawning season is winding down in Fidalgo. I'll be interested to see what the next tidal cycle does. Also yesterday I had a problem with the Hobo Shuttle. It would not read the logger pendant. I brought the logger pendant and shuttle with me to make sure neither of them was screwed up. Today I retried the pendant with the shuttle and the data offload properly. I think what happened yesterday was the shuttle go hot sitting in the sun in my tool box. The air was pretty stagnant and it got much hotter than expected. I'll double check everything on Monday to make sure the file and shuttle are in working order.

Numbers as Follow:

Temps in C

Pretreatment * Lacking the tarp and with little breeze, the trays changed temps faster than expected.
Initial  10
45      15
1.5     15

Treatment
Initial     13
45         13
1.5        14
2.25      14

Recovery
Initial    15
45        15
1.5       15

Salinity in ppt
Pretreatment    27
Treatment        60
Recovery         28


Brood Collection

2N5-8
Brood    0
Gaping   28
Dead      0
Closed   68

2S9-12
Brood     3
Gaping    45
Dead       0
Closed    38

Brooder
#    size    sick
1    30      W
2    35      W
3    33      W

2H13-16
Brood   0
Gaping  84
Dead     0
Closed  2

As for Pics. There were so few treated in the North pop that the tube label and calipers are on the tile with the gapers and the rest of the tiles are closed. The South pop tray is like normal with the brooders between the tube label and calipers. The Dabob tray had so many gapers that they are on all tiles but the closed guys are above the tube and calipers.

My face when treatments don't work.

7 31 2014 oyster bay repro check

Kamilche shores, wa

Hi 60s to low 80s sunny

Participants: Sean Bennett and Jake heare

Performed anesthesia sop with insulation on treatment tub and ice blocks on recovery tubs.  Found very few brooders again. I think it is a stack effect due to the unholy amounts of mussels set on the trays and oysters. Tried to clean them off but too many. Mussels probably consuming all sperm in water column.

Numbers as follow

Temps in c
Pretreatment
Initial.       17
45.          18
1.5.           22

Treatment
Initial.      15
45.         14
1.5.         15
2.25.       16

Recovery
Initial.     18
45.         20
1.5.         21

Salinity in ppt
Pretreatment.      26
Treatment.         60
Recovery.           27

Brood collection

1N5-8
Brood.      1
Gaping.     50
Dead.        0
Closed.      0

Brooders
#   size.   Sick
1.     30.     W.    Partial sample due to spillage.

1S13-16
Brood.    0
Gaping.   70
Dead.      0
Closed.    2

1H1-4
Brood.      1
Gaping.     59
Dead.        2
Closed.     8

Brooders
#     size.   Sick
1.       32.      Grey

7 30 2014 Manchester Repro Check

Manchester WA

Hi 60's to low 80's

Performed the anesthesia SOP with insulation on the treatment tub and water addition to the pretreatment and recovery tubs. Found one brooder in Southern and Dabob populations each. Also talked to Stuart at PSRF about spawning groups, he said they spawn Olys in groups of twenty.

Numbers as Follow

Temps in C

Pretreatment

Initial    11
45        12
1.5       14

Treatment
Initial    13
45        13
1.5       13
2.25     13

Recovery
Initial     13
45         15
1.5        17

Salinity in ppt

Pretreatment   28
Treatment       60
Recovery        27

Brood Collection

4S13-16
Brood   1
Gaping  69
Dead     10
Closed   10

Brooder
#   Size  Sick
1    24    W   *Sample of poor quality due to screen failure

4N1-4
Brood     0
Gaping    45
Dead      12
Closed    7

4H9-12
Brood     1
Gaping    45
Dead       9
Closed    11

Brooder
#     size     sick
1     20       w

Pics have brooders between tube label and caliper. Closed oysters on the same tile. Open oysters on the other three tiles. Some oysters on closed/brooder tiles are open and vice versa due to the fact of the guys glued to the tiles.

7 28 2014 Temp Data Manipulation

I attempted to manipulate the temp data today. I'm running into the same sed error on the windows computers that jammed me up back in the winter. I can edit pretty much anything and produce an out file but for some reason if I try to eliminate the quotation marks using sed so that I can have clean column heading, sed will not produce an outfile or even change the file its working on. It shows the edit in the notebook but then doesn't create the actual change in the file. Does anyone know how to solve this other than bouncing back and forth between my linux computer and desktop.

See attached notebook

7 25 2014 Fidalgo repro check

Anacortes wa

Mid 60s to mid 70s sunny

Performed the anesthesia sop with insulation on treatment tub and a tarp on the Pretreatment and recovery tubs. Treatment less effective than normal but still had an acceptable amount of Gaping oysters. Found many grey sick animals in the south pop.

Numbers as follow :

Temps in c
Pretreatment
Initial.          9
45.             11
1.5.             11

Treatment.
Initial.        11
45.           11
1.5.            11
2.25.         11

Recovery
Initial.         11
45.            12
1.5.            14

Salinity in ppt
Pretreatment.      27
Treatment.          63
Recovery.           25

Brood collection
2N9-12
Brood.      0
Gaping.     55
Dead.        0
Closed.      45

2H1-4
Brood.       2
Gaping.      50
Dead.         0
Closed.      36

Brooders
#     size.   Sick
1.       20.      W
2.      22.       W

2S5-8
Brood.     6
Gaping.    72
Dead.       0
Closed.    25

Brooders
#     size.    Sick
1.       29.      Grey
2.      25.      Grey
3.      30.      Grey
4.      30.      W
5.      32.      W
6.      31.       Grey

Oyster below calipers closed. Oysters between calipers and label tube brooders.

7 24 2014. Oyster bay repro check

Oyster bay wa

High 60s to low 70s partly cloudy

Performed the anesthesia sop with insulation on treatment tub and a tarp on the Pretreatment and recovery tubs. Brooding back to normal with most brooders in southern pop, and equal numbers between north and dabob. Also crab fresh cleaning salt water lines used their water for Pretreatment but not for recovery because pump was shut down.

Numbers as follow
Temps in c

Pretreatment
Initial.    15
45.        15
1.5.        15

Treatment
Initial.    12
45.        12
1.5.        12
2.25.     13

Recovery
Initial.    13
45.        13
1.5.        14

Brood collection

1S1-4
Brood.      8
Gaping.     75
Dead.        4
Closed.     7

Brooders
#    size.    Sick
1.      24.      W
2.     34.       W
3.     30.       W
4.     30.      W
5.     30.      W
6.     25.      W
7.     26.      W
8.     27.       W

1N9-12
Brood.       3
Gaping.     68
Dead.        0
Closed.      10

Brooders
#     size.     Sick
1.      33.        W
2.     28.        W
3.     30.        W

1H9-12
Brood.     4
Gaping.    72
Dead.       4
Closed.    6

Brooders
#      size.      Sick
1.        25.        W
2.       26.        W
3.       28.        W
4.       25.        W.    No sample.

Also pics are of whole tray. Brooders between tube and calipers or bag. Closed same time outside of brooders. Rest are gapers.