Wednesday, July 30, 2014
7 30 2014 Manchester Repro Check
Hi 60's to low 80's
Performed the anesthesia SOP with insulation on the treatment tub and water addition to the pretreatment and recovery tubs. Found one brooder in Southern and Dabob populations each. Also talked to Stuart at PSRF about spawning groups, he said they spawn Olys in groups of twenty.
Numbers as Follow
Temps in C
Pretreatment
Initial 11
45 12
1.5 14
Treatment
Initial 13
45 13
1.5 13
2.25 13
Recovery
Initial 13
45 15
1.5 17
Salinity in ppt
Pretreatment 28
Treatment 60
Recovery 27
Brood Collection
4S13-16
Brood 1
Gaping 69
Dead 10
Closed 10
Brooder
# Size Sick
1 24 W *Sample of poor quality due to screen failure
4N1-4
Brood 0
Gaping 45
Dead 12
Closed 7
4H9-12
Brood 1
Gaping 45
Dead 9
Closed 11
Brooder
# size sick
1 20 w
Pics have brooders between tube label and caliper. Closed oysters on the same tile. Open oysters on the other three tiles. Some oysters on closed/brooder tiles are open and vice versa due to the fact of the guys glued to the tiles.
Monday, July 28, 2014
7 28 2014 Temp Data Manipulation
See attached notebook
Friday, July 25, 2014
7 25 2014 Fidalgo repro check
Anacortes wa
Mid 60s to mid 70s sunny
Performed the anesthesia sop with insulation on treatment tub and a tarp on the Pretreatment and recovery tubs. Treatment less effective than normal but still had an acceptable amount of Gaping oysters. Found many grey sick animals in the south pop.
Numbers as follow :
Temps in c
Pretreatment
Initial. 9
45. 11
1.5. 11
Treatment.
Initial. 11
45. 11
1.5. 11
2.25. 11
Recovery
Initial. 11
45. 12
1.5. 14
Salinity in ppt
Pretreatment. 27
Treatment. 63
Recovery. 25
Brood collection
2N9-12
Brood. 0
Gaping. 55
Dead. 0
Closed. 45
2H1-4
Brood. 2
Gaping. 50
Dead. 0
Closed. 36
Brooders
# size. Sick
1. 20. W
2. 22. W
2S5-8
Brood. 6
Gaping. 72
Dead. 0
Closed. 25
Brooders
# size. Sick
1. 29. Grey
2. 25. Grey
3. 30. Grey
4. 30. W
5. 32. W
6. 31. Grey
Oyster below calipers closed. Oysters between calipers and label tube brooders.
Thursday, July 24, 2014
7 24 2014. Oyster bay repro check
Oyster bay wa
High 60s to low 70s partly cloudy
Performed the anesthesia sop with insulation on treatment tub and a tarp on the Pretreatment and recovery tubs. Brooding back to normal with most brooders in southern pop, and equal numbers between north and dabob. Also crab fresh cleaning salt water lines used their water for Pretreatment but not for recovery because pump was shut down.
Numbers as follow
Temps in c
Pretreatment
Initial. 15
45. 15
1.5. 15
Treatment
Initial. 12
45. 12
1.5. 12
2.25. 13
Recovery
Initial. 13
45. 13
1.5. 14
Brood collection
1S1-4
Brood. 8
Gaping. 75
Dead. 4
Closed. 7
Brooders
# size. Sick
1. 24. W
2. 34. W
3. 30. W
4. 30. W
5. 30. W
6. 25. W
7. 26. W
8. 27. W
1N9-12
Brood. 3
Gaping. 68
Dead. 0
Closed. 10
Brooders
# size. Sick
1. 33. W
2. 28. W
3. 30. W
1H9-12
Brood. 4
Gaping. 72
Dead. 4
Closed. 6
Brooders
# size. Sick
1. 25. W
2. 26. W
3. 28. W
4. 25. W. No sample.
Also pics are of whole tray. Brooders between tube and calipers or bag. Closed same time outside of brooders. Rest are gapers.
Wednesday, July 23, 2014
7 23 14 Manchester Repro Survey
Hi 50's to mid 60's Rainy, overcast
Participants: Joseph Stevick and Jake Heare
Performed the anesthesia sop with insulation on the treatment tub and tarp on the pretreatment/recovery tubs. No ice blocks because of the cloudy cool conditions. Found a few brooders in the Southern pop and one in the Dabob pop. Very middle of the road spawning.
Numbers as follow
Temp in C
Pretreatment
initial 8
45 min 9
1.5 hr 8
Treatment
Initial 8
45 min 9
1.5 hr 8
2.25 hr 8
Recovery
Initial 8
45 min 8
1.5 hr 8
Salinity in ppt
Pretreatment 27
Treatment 70
Recovery 28
Brood collection
4H5-8
Brood 1
Gaping 67
dead 7
closed 9
brooders
# size sick
1 15 w
4S1-4
Brood 2
Gaping 59
Dead 1
closed 6
Brooders
# size sick
1 27 w
2 23 w
4N5-8
Brood 0
Gaping 60
Dead 10
Closed 9
Also took pictures of the entire population. In the pics, the tile with the caliper and label tube are the closed animals except for the animals that are between the tube and calipers which were brooders.
Friday, July 18, 2014
7 18 2014 fidalgo bay repro check
Anacortes, WA
Mid 60s to mid 70s
Participants: alicia Godersky and Jake heare
Performed anesthesia sop with insulation on treatment tub. Found brooders in only the dabob pop. It seems that they spawn later in the season but in smaller number. We will see in the coming weeks. Also treatment was surprisingly ineffective with no discernable reason. Water was calm. Temps were stable. No movement while in tubs. Full treatment strength and time. Hopefully it was a fluke.
Numbers as follow:
Temps in c
Pretreatment
Initial. 10
45. 11
1.5. 11
Treatment
Initial. 10
45. 10
1.5. 11
2.25. 11
Recovery
Initial. 10
45. 11
1.5. 12
Salinity in ppt
Pretreatment. 28
Treatment. 66
Recovery. 28
Brood collection
2S1-4
Brood. 0
Gaping. 67
Dead. 0
Closed. 30
2H9-12
Brood. 2
Gaping. 32
Dead. 0
Closed. 50
Brooders
# size. Sick
1. 19. W
2. 20. W
2N13-16
Brood. 0
Gaping. 68
Dead. 0
Closed. 40
Thursday, July 17, 2014
7 17 2014 oyster bay repro
Oyster bay
High 60s to high 70s
Participants: alicia Godersky and Jake heare
Performed anesthesia sop with insulation on treatment and tarp with ice blocks on recovery tubs. Very heavy mussel set on all trays and oysters. Possibly causing spawning issues. Only one population had brooders which is very different than in previous weeks. The one pop was the dabob pop with 5 brooders, one of which looked like aborted spawn.
Numbers as follow.
Temps in c
Pretreatment
Initial. 16
45. 18
1.5. 18
Treatment
Initial. 13
45. 14
1.5. 14
2.25. 14
Recovery
Initial. 16
45. 16
1.5. 17
Salinity in ppt
Pretreatment. 24
Treatment. 66
Recovery. 25
Brood collection
1S13-16.
Brood. 0
Gaping. 75
Dead. 1
Closed. 2
1N5-8
Brood. 0
Gaping. 48
Dead. 1
Closed. 1
1H1-4
Brood. 5.
Gaping. 68
Dead. 6
Closed. 1
Brooders
# size. Sick
1. 30. W
2. 20. W
3. 26. W
4. 26. W
5. 27. W. Possibly abort spawn.
Wednesday, July 16, 2014
7 16 2014 Manchester Repro Check
Low 70s to Mid 80s
Participants: Alicia Godersky and Jake Heare
Performed the anesthesia SOP with insulation and ice blocks on the treatment group and tarp over the recovery and pretreatment tubs. Finally found multiple brooders in one population. If you can guess the pop, you can consider yourself an Oly Whisperer. The brooding animals were also much smaller than expected. Half were less than 20 mm which is quite odd. Also temps fluxed on the tubs on the dock even with replacing water, the combination of sun and no breeze just baked the docks.
Numbers as follow:
Temps in C
Pretreatment
Initial 10
45 12
1.5 13
Treatment
Initial 12
45 13
1.5 12
2.25 11
Recovery
Initial 12
45 14
1.5 15
Salinity in ppt
Pretreatment 26
Treatment 66
Recovery 25
Brood Collection
4S5-8
Brood 3
Gaping 59
Dead 12
Closed 12
Brooders
# size sick
1 22 w
2 16 w
3 20 w
4H13-16
Brood 1
Gaping 56
Dead 8
Closed 17
Brooders
# size sick
1 17 W
4N13-16
Brood 0
Gaping 61
Dead 6
Closed 8
Friday, July 11, 2014
7 11 2014 fidalgo repro check
Anacortes, wa
Low 70s to mid 80s
Performed the anesthesia sop with insulation and ice blocks on treatment and tarp cover on Pretreatment and recovery. Found lots of brooders in southern pop and none in the other two pops. South sound oysters seem to be very attune to tidal changes. Also temps remained within range using the tarp cover. I think I should add a board or stand to keep the tarp higher over the tubs to keep it out of the water and allow for air flow to keep air temps lower. Also note to self, next week I absolutely have to refill my EtOH, 50 ml falcon tubes, and zipties. Also need a new water proof write in the rain pen as I have lost my only good one.
Numbers as follow :
Temps in c
Pretreatment
Initial. 16
45. 16
1.5. 17
Treatment
Initial. 14
45. 15
1.5. 14
2.25. 14
Recovery
Initial. 16
45. 16
1.5. 17
Salinity
Pretreatment. 20
Treatment. 68
Recovery. 23
Brood collection
2H5-8
Brood. 0
Gaping. 70
Dead. 0
Closed. 28
2S13-16
Brood. 11
Gaping. 81
Dead. 0
Closed. 11
Brooders
# size. Sick
1. 27. W
2. 25. W
3. 26. W
4. 30. W
5. 28. W
6. 30. W
7. 27. W
8. 31. W
9. 25. W
10. 32. W
11. 31. W
2N1-4
Brood. 0
Gaping. 78
Dead. 0
Closed. 17
Thursday, July 10, 2014
7 10 2014 oyster bay repro check
Klamiche shores, wa
Hi 60s to low 80s
Performed the anesthesia sop with insulation and ice blocks on treat. I also covered the Pretreatment and recovery tubs with a tarp to shade them from direct sun to help moderate the water temps. It worked well along with adding fresh seawater and leftover ice blocks, keeping the trays within 3 degrees of ambient. Also I broke the one day record for number of brooders. All pops had multiple brooders with the northern pop almost being equal to the southern pop in terms of numbers. Dabob pop also performed very well. I think this has to do with last week's neap tide and the much warmer than average air temps.
Numbers as follow.
Temps in c
Pretreatment
Initial. 16
45. 16
1.5. 16
Treatment
Initial. 14
45. 14
1.5. 13
2.25. 12
Recovery
Initial. 16
45. 17
1.5. 17
Salinity in ppt
Pretreatment. 25
Treatment. 65
Recovery. 24
Brood collection
1N9-12
Brood. 8
Gaping. 73
Dead. 3
Closed. 2
Brooders
# size. Sick
1. 30. W
2. 22. W no sample, very few larvae
3. 39. W
4. 35. W
5. 31. W
6. 33. W
7. 28. W
8. 32. W no sample tile oyster
1H9-12
Brood. 6
Gaping. 83
Dead. 3
Closed. 3
Brooders
# size. Sick
1. 27. W
2. 27. W.
3. 28. W
4. 26. W
5. 26. W
6. 27. W
1S1-4
Brood. 10
Gaping. 82
Dead. 0
Closed. 1
Brooders
# size. Sick
1. 22. W
2. 22. W
3. 25. W
4. 28. W
5. 27. W
6. 27. W
7. 25. W
8. 24. W
9. 30. W
10. 30. W. No sample, tile oyster
Wednesday, July 9, 2014
7 9 2014 Manchester Repro Check
Hi 60s to Mid 80s, sunny
Participants: Joe Stevick and Jake Heare
Used the anesthesia SOP with insulation modification and ice blocks in both the treatment and recovery trays. Treatment stayed within 1 degree, while recovery trays stayed within 5 degrees. Found 1 brooder from the Dabob population which was pretty interesting considering that none of the other populations have been spawning.
Numbers as follow:
Temps in C
Pretreatment
Initial 11
45 min 13
1.5 hr 16
Treatment
Initial 11
45 min 12
1.5 hr 11
2.25 hr 10
Recovery
Initial 13
45 min 16
1.5 hr 17
Salinity in PPT
Pretreatment 26
Treatment 53
Recovery 27
Brood Collection
4N9-12
Brood 0
Gaping 45
Dead 12
Closed 2
4S9-12
Brood 0
Gaping 55
Dead 22
Closed 15
4H1-4
Brood 1
Gaping 60
Dead 5
Closed 21
Brooders
# size sick
1 21 w
Monday, July 7, 2014
7 7 2014 Running Tally of Brooders
Total Brooders | Manchester | Oyster Bay | Fidalgo |
N | 1 | 15 | 3 |
H | 1 | 8 | 1 |
S | 1 | 41 | 17 |
1st brood | 6/18/2014 | 5/29/2014 | 6/6/2014 |
Friday, July 4, 2014
7 4 2014 Fidalgo repro check
Anacortes wa
Mid 60s to low 70s
Participants: Jim cerkovnik and Jake heare
Performed anesthesia sop with insulation on treatment tub. Found brood Dr s in all three pops. Majority in southern pop, equal numbers in northern and dabob.
Numbers as follow:
Temps in c
Pretreatment
Initial. 9
45 11
1.5. 12
Treatment
Initial. 12
45. 12
1.5. 12
2.25. 12
Recovery
Initial. 13
45. 13
1.5. 15
Salinity in ppt
Pretreatment. 31
Treatment. 65
Recovery. 32
Brood collection
2H13-16
Brood. 1
Gaping. 88
Dead. 0
Closed. 9
Brooders
# size. Sick
1. 23. W
2S9-12
Brood. 6
Gaping 71
Dead. 0
Closed. 14
Brooders
# size. Sick
1. 30. W
2. 32. W
3. 32 W
4. 41. W
5. 32. Grey
6. 21. W
2N5-8
Brood. 1
Gaping. 82
Dead. 0
Closed. 13
Brooders
# size. Sick
1. 28. W
Thursday, July 3, 2014
7 3 2014 oyster bay repro check
Shelton, wa
Mid 60s to high 70s cloudy mostly till 2 pm then sunny
Performed the anesthesia sop with insulation on treatment. No ice blocks needed. Found no brooders in North pop but 3 in dabob. Lots still from south sound pop.
Numbers as follow
Temps in c
Pretreatment
Initial. 15
45. 15
1.5. 16
Treatment
Initial. 11
45. 10
1.5. 11
2.25. 11
Recovery
Initial. 16
45. 19
1.5. 22 sun came out. Kept adding water.
Salinity in ppt
Pretreatment. 25
Treatment. 73
Recovery. 26
Brood collection
1N5-8
Brood. 0
Gaping. 49
Dead. 0
Closed. 1
1H1-4
Brood. 3
Gaping. 66
Dead. 4
Closed. 4
Brooders
# size. Sick
1. 25. W
2. 26. Grey
3. 25. W
1S13-16
Brood. 9
Gaping. 78
Dead. 0
Closed. 3
Brooders
# size. Sick
1. 26. W
2. 28. W
3. 26. W
4. 30. W
5. 39. W
6. 25. W
7. 28. W
8. 29. W
9. 25. W. No larvae collected from 9. Tile oyster.
Wednesday, July 2, 2014
7 2 2014 Manchester Repro Update
Mid 60's to High 70s Sunny
Participants: L. Christine Savolainen and Jake Heare
Used the Anesthesia SOP with the insulation and ice blocks on the treatment tubs. Found absolutely no brooders. This site just does not have the conditions necessary for these animals to spawn. It must be due to the colder deep water that comes into the bay off the Sound.
Numbers as Follow:
Temps in C
Pretreatment
Initial 10
45 min 11
1.5 hr 15 * sun came out
Treatment
Initial 11
45 min 11
1.5 hr 10
2.25 9
Recovery
Initial 11
45 min 13
1.5 hr 15
Salinity in ppt
Pretreatment 28
Treatment 67
Recovery 28
Brood Collection
4H9-12
Brood 0
Gaping 52
Dead 20
Closed 8
4S13-16
Brood 0
Gaping 31
Dead 4
Closed 48 *Lots of boat traffic shaking the dock
4N1-4
Brood 0
Gaping 40
Dead 20
Closed 18
Monday, June 30, 2014
6 30 2014 June Survivorship Data
All numbers are for living animals only.
Oyster Bay | N | H | S |
1-4 | 73 | 86 | 97 |
5-8 | 63 | 56 | 0 |
9-12 | 85 | 97 | 1 |
13-16 | 0 | 12 | 93 |
Total | 221 | 251 | 191 |
Manchester | N | H | S |
1-4 | 90 | 96 | 65 |
5-8 | 95 | 92 | 93 |
9-12 | 61 | 89 | 98 |
13-16 | 87 | 79 | 94 |
Total | 333 | 356 | 350 |
Fidalgo | N | H | S |
1-4 | 96 | 93 | 93 |
5-8 | 97 | 99 | 93 |
9-12 | 93 | 87 | 97 |
13-16 | 110 | 91 | 87 |
Total | 396 | 370 | 370 |
Total Brooders | Manchester | Oyster Bay | Fidalgo |
N | 1 | 15 | 2 |
H | 1 | 5 | 0 |
S | 1 | 32 | 11 |
1st brood | 6/18/2014 | 5/29/2014 | 6/6/2014 |
*1N trays were sampled twice in one sample date |
Percent Brood | Manchester | Oyster Bay | Fidalgo |
N | 0.30% | 6.79% | 0.51% |
H | 0.28% | 1.99% | 0.00% |
S | 0.29% | 16.75% | 2.97% |
6 30 2014 South Sound Survivors
Pop Live
North 221
Dabob 251
South 191
Tray Break Down is such:
South Sound | |||
N | H | S | |
1-4 | 73 | 86 | 97 |
5-8 | 63 | 56 | 0 |
9-12 | 85 | 97 | 1 |
13-16 | 0 | 12 | 93 |
Total | 221 | 251 | 191 |
Friday, June 27, 2014
6 27 2014 fidalgo repro check
Initial. 9
45. 10
1.5. 10
Initial. 9
45. 10
1.5. 10
2.25. 12
Initial. 10
45. 10
1.5. 14
Pretreatment. 29
Treatment. 58
Recovery. 28
Brood. 3
Gaping. 87
Dead. 0
Closed. 6
# size. Sick
1. 27. W
2. 25. W
3. 26. W
Brood. 0
Gaping. 65
Dead. 0
Closed. 28
Brood. 1
Gaping. 87
Dead. 0
Closed. 6
# size. Sick
1. 31. W