Showing posts with label Fidalgo. Show all posts
Showing posts with label Fidalgo. Show all posts

Friday, August 15, 2014

8 15 14 fidalgo repro check

Anacortes wa
High 50s to low 70s mostly cloudy light rain early dry later
Performed the anesthesia sop with insulation on treatment tub. weather allowed to go without ice blocks or tarp. Found no brooders. Thought that was weird. Checked previous entries and realized I treated the same stack twice in a row due to an error I made last week. Not sure if it really signifies the end of spawning or if I'm really efficient at collecting larvae.

Numbers as follow

Temps in c
Pretreatment
Initial.   12
45.       12
1.5.       13
Treatment
Initial.    12
45.        12
1.5.        12
2.25.      13
Recovery
Initial.     12
45.         13
1.5.         14

Salinity in ppt
Pretreatment.   25
Treatment.       63
Recovery.        24

Brood collection

2N13-16
Brood.    0
Gaping.   78
Dead.      0
Closed.    25

2H9-12
Brood.    0
Gaping.   83
Dead.      0
Closed.    3

2S1-4
Brood.   0
Gaping.  88
Dead.     0
Closed.   6
Pics have closed on same tile as tube label and calipers. Gapers on all other tiles.

Friday, August 8, 2014

8 8 2014 fidalgo repro check

Anacortes wa

Mid 60s to high 70s sunny

Performed the anesthesia sop with insulation on treatment tub and a tarp on the Pretreatment and recovery tubs. Treatment was effective as normal so it seemed to be a fluke the last couple of weeks. Also had pretty equal brooding across all populations. Finally the northern population had the most number of brooders. I think we should check these animals again around the full moon in September to see if they are still spawning.

Numbers as follow :

Temps in c
Pretreatment
Initial.   11
45.      13
1.5.       15

Treatment
Initial.     13
45.        14
1.5.         13
2.25.       14

Recovery
Initial.     11
45.        12
1.5.         15

Salinity in ppt
Pretreatment.       27
Treatment.           56
Recovery.            28

Brood collection
2S1-4
Brood.      2
Gaping.     70
Dead.        0
Closed.      22

Brooders
#   size.  Sick
1.     32.    Grey
2.     30.    W

2H9-12
Brood.       3
Gaping.      67
Dead.         0
Closed.      15

Brooders
#     size.     Sick
1.      30.       W
2.     29.        W
3.      20.      W

2N13-16
Brood.     5
Gaping.    89
Dead.       0
Closed.    10

Brooders
#   size.   Sick
1.     19.      W
2.    34.      W
3.    35.      W
4.    32.      W
5.    29.      W

Pics have brooders between Tube and calipers. Closed on same tile. Gaping on all other tiles.

Friday, August 1, 2014

8 1 2014 Fidalgo Repro Check

Anacortes WA

Mid 60s to high 70s. Sunny until 2 pm then partly cloudy

Performed the anesthesia SOP with insulation on the treatment tub and ice blocks in the Treatment and recovery tubs to stabilize temperature fluctuations. Had a really poor treatment of the north sound tray and a less than great treatment of the south pop tray. Less than a third of the animals were gaping in north pop and only a little over half in the South pop were open. The Dabob tray was over 95% treated. I'm not sure we missed any brooders though as South sound only had a few and Dabob had none. I'm not sure why the animals didn't open but I assume that it might be due to the fact that they were quickly sprayed with fresh water to help knock off some of the more intense sediment deposits on them. This great to know for next time so I don't accidentally deter treatment. It seems like spawning season is winding down in Fidalgo. I'll be interested to see what the next tidal cycle does. Also yesterday I had a problem with the Hobo Shuttle. It would not read the logger pendant. I brought the logger pendant and shuttle with me to make sure neither of them was screwed up. Today I retried the pendant with the shuttle and the data offload properly. I think what happened yesterday was the shuttle go hot sitting in the sun in my tool box. The air was pretty stagnant and it got much hotter than expected. I'll double check everything on Monday to make sure the file and shuttle are in working order.

Numbers as Follow:

Temps in C

Pretreatment * Lacking the tarp and with little breeze, the trays changed temps faster than expected.
Initial  10
45      15
1.5     15

Treatment
Initial     13
45         13
1.5        14
2.25      14

Recovery
Initial    15
45        15
1.5       15

Salinity in ppt
Pretreatment    27
Treatment        60
Recovery         28


Brood Collection

2N5-8
Brood    0
Gaping   28
Dead      0
Closed   68

2S9-12
Brood     3
Gaping    45
Dead       0
Closed    38

Brooder
#    size    sick
1    30      W
2    35      W
3    33      W

2H13-16
Brood   0
Gaping  84
Dead     0
Closed  2

As for Pics. There were so few treated in the North pop that the tube label and calipers are on the tile with the gapers and the rest of the tiles are closed. The South pop tray is like normal with the brooders between the tube label and calipers. The Dabob tray had so many gapers that they are on all tiles but the closed guys are above the tube and calipers.



My face when treatments don't work.

Friday, July 25, 2014

7 25 2014 Fidalgo repro check

Anacortes wa

Mid 60s to mid 70s sunny

Performed the anesthesia sop with insulation on treatment tub and a tarp on the Pretreatment and recovery tubs. Treatment less effective than normal but still had an acceptable amount of Gaping oysters. Found many grey sick animals in the south pop.

Numbers as follow :

Temps in c
Pretreatment
Initial.          9
45.             11
1.5.             11

Treatment.
Initial.        11
45.           11
1.5.            11
2.25.         11

Recovery
Initial.         11
45.            12
1.5.            14

Salinity in ppt
Pretreatment.      27
Treatment.          63
Recovery.           25

Brood collection
2N9-12
Brood.      0
Gaping.     55
Dead.        0
Closed.      45

2H1-4
Brood.       2
Gaping.      50
Dead.         0
Closed.      36

Brooders
#     size.   Sick
1.       20.      W
2.      22.       W

2S5-8
Brood.     6
Gaping.    72
Dead.       0
Closed.    25

Brooders
#     size.    Sick
1.       29.      Grey
2.      25.      Grey
3.      30.      Grey
4.      30.      W
5.      32.      W
6.      31.       Grey

Oyster below calipers closed. Oysters between calipers and label tube brooders.

Friday, July 18, 2014

7 18 2014 fidalgo bay repro check

Anacortes, WA

Mid 60s to mid 70s

Participants: alicia Godersky and Jake heare

Performed anesthesia sop with insulation on treatment tub.  Found brooders in only the dabob pop. It seems that they spawn later in the season but in smaller number. We will see in the coming weeks. Also treatment was surprisingly ineffective with no discernable reason. Water was calm. Temps were stable. No movement while in tubs. Full treatment strength and time.  Hopefully it was a fluke.

Numbers as follow:

Temps in c
Pretreatment
Initial.     10
45.         11
1.5.         11

Treatment
Initial.   10
45.       10
1.5.       11
2.25.     11

Recovery
Initial.      10
45.          11
1.5.          12

Salinity in ppt
Pretreatment.       28
Treatment.           66
Recovery.            28

Brood collection

2S1-4
Brood.      0
Gaping.     67
Dead.        0
Closed.     30

2H9-12
Brood.      2
Gaping.    32
Dead.       0
Closed.     50

Brooders
#     size.     Sick
1.       19.        W
2.      20.       W

2N13-16
Brood.     0
Gaping.    68
Dead.       0
Closed.    40

Friday, July 11, 2014

7 11 2014 fidalgo repro check

Anacortes, wa

Low 70s to mid 80s

Performed the anesthesia sop with insulation and ice blocks on treatment and tarp cover on Pretreatment and recovery.  Found lots of brooders in southern pop and none in the other two pops. South sound oysters seem to be very attune to tidal changes. Also temps remained within range using the tarp cover. I think I should add a board or stand to keep the tarp higher over the tubs to keep it out of the water and allow for air flow to keep air temps lower. Also note to self, next week I absolutely have to refill my EtOH, 50 ml falcon tubes, and zipties. Also need a new water proof write in the rain pen as I have lost my only good one.

Numbers as follow :

Temps in c
Pretreatment
Initial.   16
45.         16
1.5.        17

Treatment
Initial.          14
45.                15
1.5.               14
2.25.             14

Recovery
Initial.         16
45.               16
1.5.              17

Salinity
Pretreatment.         20
Treatment.              68
Recovery.               23

Brood collection

2H5-8
Brood.     0
Gaping.   70
Dead.       0
Closed.    28

2S13-16
Brood.       11
Gaping.     81
Dead.         0
Closed.      11

Brooders
#    size.    Sick
1.    27.       W
2.    25.       W
3.    26.       W
4.    30.       W
5.    28.       W
6.    30.       W
7.    27.       W
8.    31.       W
9.    25.       W
10.  32.       W
11.  31.       W

2N1-4
Brood.      0
Gaping.    78
Dead.        0
Closed.     17

Friday, July 4, 2014

7 4 2014 Fidalgo repro check

Anacortes wa

Mid 60s to low 70s

Participants: Jim cerkovnik and Jake heare

Performed anesthesia sop with insulation on treatment tub. Found brood Dr s in all three pops. Majority in southern pop, equal numbers in northern and dabob.

Numbers as follow:

Temps in c
Pretreatment
Initial.        9
45              11
1.5.            12

Treatment
Initial.        12
45.              12
1.5.             12
2.25.           12

Recovery
Initial.          13
45.                13
1.5.               15

Salinity in ppt
Pretreatment.       31
Treatment.            65
Recovery.              32

Brood collection
2H13-16
Brood.     1
Gaping.   88
Dead.       0
Closed.    9

Brooders
#         size.     Sick
1.         23.         W

2S9-12
Brood.      6
Gaping     71
Dead.        0
Closed.     14

Brooders
#     size.     Sick
1.     30.        W
2.     32.         W
3.     32         W
4.      41.        W
5.       32.      Grey
6.       21.       W

2N5-8
Brood.      1
Gaping.     82
Dead.        0
Closed.      13

Brooders
#     size.     Sick
1.       28.      W
 

Monday, June 30, 2014

6 30 2014 June Survivorship Data

Just for toots and googles I worked up the survivorship data from June just to see where we stand number wise out in the field. This comes from the weekly counts of gapers and closed animals which together make up the total number of living animals. These numbers are tentative and will be verified in the future when we do a full scale work up of the samples in August or September for the annual sample taking.

All numbers are for living animals only.

Oyster BayNHS
1-4738697
5-863560
9-1285971
13-1601293
Total221251191
ManchesterNHS
1-4909665
5-8959293
9-12618998
13-16877994
Total333356350
FidalgoNHS
1-4969393
5-8979993
9-12938797
13-161109187
Total396370370

While there has been a pretty major loss in Oyster Bay, I think we can safely proceed into year 2 with enough animals to get significant data from their development as well as Year 2 reproductive effort. 

Also in previous counts Manchester had the highest survival but is now well below that of Fidalgo. I think this is due to the much warmer sunnier conditions at Manchester which lead to a 10% mortality event in the beginning of May and may continue to cause a small 1-2% mortality each month during repro sampling. 

This info is also really cool when you compare it to the number of Brooders I've found over the course of the season for each population at each site. 

Total BroodersManchesterOyster BayFidalgo
N1152
H150
S13211
1st brood6/18/20145/29/20146/6/2014
*1N trays were sampled twice in one sample date
Percent brooders from each pop for entirety of sampling period is as follows

Percent BroodManchesterOyster BayFidalgo
N0.30%6.79%0.51%
H0.28%1.99%0.00%
S0.29%16.75%2.97%

So almost 17% of the South Sound population at Oyster Bay has spawned and about 3% of the same population at Fidalgo has spawned. It blows me away that they are nearly 6 times as productive as the Dabob population in their home site but at Manchester they are not significantly different.  Its also cool that they are twice as productive as the Northern population in South sound and almost 5 times more productive at Fidalgo bay. 

Bonnie has suggested to me that the animals taken from Fidalgo for the original broodstock are possibly from a population that is being restored with animals from South Sound. This could be leading to an outbreeding depression with regards to local adaptation in the northern population and the reason the South sound animals are more prolific than the northern pops. 

The other take away from these numbers is that it seems to me that Dabob animals are juggernauts that don't spawn. They have the highest survivorship at two of the sites (though Oyster Bay is arguable due to the many accidental exposure and other mishaps) and are very close to the North Sound populace at Fidalgo. 

Eventually graphing all this with temps and tide times would be helpful to determine any differences in week to week spawn collections. 

Friday, June 27, 2014

6 27 2014 fidalgo repro check

Anacortes wa
Mid 50 to mid 60s

Participants:  Sean Bennett and Jake heare

Followed the anesthesia sop modified with insulation on bottom of tub. Too windy to put cover on, also forgot to get ice. Cool enough to keep treatment with 3 degrees. Found brooders in south sound and north sound pop but none in dabob still.

Numbers as follow:

Temps in c
Pretreatment
Initial.         9
45.              10
1.5.             10

Treatment
Initial.            9
45.                 10
1.5.                10
2.25.              12

Recovery
Initial.          10
45.                10
1.5.               14

Salinity in ppt
Pretreatment.           29
Treatment.                58
Recovery.                 28

Brood collection
2S5-8
Brood.          3
Gaping.        87
Dead.            0
Closed.        6
Brooders
#         size.         Sick
1.         27.             W
2.         25.             W
3.         26.             W

2H1-4
Brood.       0
Gaping.     65
Dead.         0
Closed.      28

2N9-12
Brood.      1
Gaping.    87
Dead.        0
Closed.     6
Brooders
#         size.         Sick
1.         31.            W

Friday, June 20, 2014

6 20 2014 fidalgo repro check

Anacortes wa
Mid 60s cloudy to sunny
Participants: L. Christine savolainen and Jake heare
Followed the anesthesia sop with insulation on the treatment group.  Found one brooder from the southern population. Two animals looked like possible brooders in the northern pop but they closed quickly and I was unable to confirm brooding in them.
Also Brian found the missing stack at oyster bay. They will be worked up next Thursday as they are safely secured to the dock again.
Numbers as follows
Temps in c
Pretreatment.
Initial.       8
45 min.     8
1.5 hrs.     9
Treatment
Initial.           9
45 min.         9
1.5 hrs.         10
2.25 hrs.       10
Recovery
Initial.        7
45 min.      8
1.5 hrs.      8
Salinity in ppt
Pretreatment.      35
Treatment.           65
Recovery.             35
Brood collection
2S1-4
Brood.       1
Gaping.     83
Dead.         0
Closed.      10
Brooders
#       size.     Sick
1.       27.        W
2H9-12
Brood.        0
Gaping.      77
Dead.          0
Closed.       10
2N13-16
Brood.     0
Gaping.    76
Dead.       0
Closed.    34

Monday, June 16, 2014

Temperature Logger Readings from May to Mid June 2014

Last week I pulled temperature data from the logger pendants out in the field using the Shuttle. Everything performed stellarly and I have the temp graphs on the computer. I'll go by site for each temp graph.

Manchester, WA


From the temperature readings it appears that Manchester has stayed below the 12.5 C mark for most of the logging period. Only recently have temperatures crested the 12.5 mark and stayed that way for several days. It may be soon that the animals will begin spawning and brooding (or spawned within the last week or so). 

Oyster Bay, WA


From this data and the amount of brooders we have seen at Oyster Bay, its clear that temps have been above the 12.5 C mark  since early May. This again correlates with expectations and as temperatures steadily increase I believe we may see a slowing of reproduction due to stress demands. 

Fidalgo Bay, WA


Contrary to the observations in the field, it seems that Fidalgo bay has been much warmer since the beginning of June. Though it does look like in early May there was a warm period for several days. As of June 1st temperatures crested the 12.5 C mark and headed into spawning temps. We saw our first brooder approximately 6 days after that and saw a large number of brooders approximately 12 days after temps went above 12.5. Though temps have somewhat decreased recently I believe we will continue to see spawning across the board.


Overall I think that the populations are responding to temperature cues nicely, though it does seem that North sound respond quickly to temperature increases. The south sound animals seem to have nearly synchronous spawning during warm periods which would be of obvious benefit in South Sound where temps continue to increase and only stay within the spawning window for a short amount of time. The dabob animals seem to have reduced response to temperature cues and do not actively spawn unless temps are somewhat stable for a duration of time. 


Friday, June 13, 2014

6 13 2014. Fidalgo repro check

Anacortes wa

Mid 60s partly cloudy lite rain

Participants:  Sean Bennett and Jake Heare

Used the anesthesia sop with insulation on the treatment group. Temps stayed steady due to insulation and weather. Found multiple brooders in only one population. If you can guess which pop, you get a sticker.

Numbers as follow:

Temps in c

Pretreatment
Initial.         7
45 min.       7
1.5 hrs.       10

Treatment
Initial.          6
45 min.        6
1.5 hrs.         6
2.25 hrs.       6

Recovery
Initial.             7
45 min.           8
1.5 hrs.           9

Salinity in ppt
Pretreatment.     27
Treatment.           66
Recovery.             26

2N1-4
Brood.       0
Gaping.     68
Dead.         1
Closed.       27

2S13-16
Brood.     7
Gaping     83
Dead.       1
Closed.     3

Brooders
#           size.              Sick
1.            30.                W
2.             26.                W
3.             26.                W
4.              25.              W
5.              26.               W
6.              28.               W
7.              25.              W

2H5-8
Brood.         0
Gaping.       72
Dead.           0
Closed.        27

Lots of males with semen. Expect more brooders next week.

Friday, June 6, 2014

6 6 2014 fidalgo repro check

Followed the anesthesia sop at fidalgo. Attempted to keep the treatment Temps around 11 c where the ambient water was. The direct sun had other plans. Will need way way way more gel bags. even then it might be more than can actually fit in the tub with the samples.

Also to my and hopefully everyone else's surprise. I found a Brooding oyster! If you can guess which population it came from before you look below, you are one smart cookie.

Numbers as follow

Temps
Pretreatment
Initial.      10
45 min.     15
1.5 hrs.      19

Treatment
Initial.         11
45 min.       16
1.5 hrs.        17
2.25 hrs.      19

Recovery
Initial     10
45 min.   15
1.5 hrs.    19

Salinity
Pretreatment.     20
Treatment.           63
Recovery.             21

Brood collection
2S9-12
Brood.      0
Gaping.    53
Dead.        1
Closed.      43

2H13-16
Brood.       0
Gaping.     58
Dead.         0
Closed.       33

2N5-8
Brood.      1
Gaping.    59
Dead.        2
Closed.      36

Brooders
#         size(mm).        Sick
1.          31.                     W

Lots of nukaluka in the water. Some may have been collected with brooding larvae. May muddy up counting.

Picture of brooders follows.

Friday, May 30, 2014

5 30 2014 fidalgo repro check

Anacortes wa
Mid 60s to mid 70s
Performed standard reproduction check using the anesthesia sop. Found no brooders. Also surface water Temps at 3 pm were only 9C.
Temps
Pretreatment
Initial 10
45 min. 12
1.5 hrs   16
Treatment
Initial. 9
45 min.  13
1.5 hrs.  16
2.25 hrs.  19
Recovery
Initial.  9
45 min. 13
1.5 hrs.  16
Salinity
Seawater.  30 ppt
Treatment.  65 ppt
Brood
2S5-8
Brood.   0
Gaping.  77
Dead.      1
2N9-12
Brood.   0
Gaping.  46
Dead.     0
2H1-4
Brood.  0
Gaping. 50
Dead.   1
Also forgot to dessicate 2N tray. Placed it straight in treatment from bath. Still got ok reaction from it.

Saturday, May 24, 2014

5 24 2014 fidalgo repro

Anacortes, WA
Temps mid 50 to 60
Light rain
Participants L. Christine savolainen and Jake heare


Checked the third stack for reproductive activities using Anesthesia SOP. Also checked the first stack to see if there were any mortality events. The first stack was completely fine with minimal mortalities.
The third stack had no signs of brooding.
Numbers for pops as follows

2H9-12
Brood. 0
Gaping. 24
Dead 0

2N13-16
Brood. 0
Gaping 53
Dead 0

2S1-4
Brood. 0
Gaping. 77
Dead. 0
#priorities

Tuesday, May 20, 2014

Capsized boat at Fidalgo Marina

Apparently a boat capsized roughly 500 feet from the samples at Fidalgo Bay. Depending on what leaked into the water, the size of the wake and other disturbances caused by rescue efforts. This might cause some issues with the animals. You can watch a time lapse video of the recovery effort here.

Monday, May 19, 2014

5-14 to 15-16 2014 Reproduction Work Up

5-14-2014

Manchester WA

Participants: Brent Vadopalas and Jake Heare

We retrieved the next set of trays in the dosing sequence and allowed each tray to dessicate for 45 minutes. Trays were then treated in a 10 gallon bath of 50% sea water to 50% freshwater mixed with 7 lbs of epsom salt. The tray was treated for 45 minutes, at which point it was removed and gaping animals were examined for signs of brooding. After examination the trays were then placed into a recovery tub with 100% sea water until the last tray examined had be in the recovery tub for 45 minutes. After each treatment the treatment water was replaced with fresh treatment to reduce temperature flux.  Then trays were rebuilt into a stack and hung off the dock. 

We counted the number of gaping animals and brooders for each tray. 

They are as follows:
4H1-4
brooders 0
Gaping 25
Closed 72
% Open  25.8%

4S9-12
Brooders 0
Gaping  43
Closed  55
% Open  43.9%

4N9-12
Brooders 0
Gaping 31
Closed  28
% Open 52.5%








5-15-2014

Oyster Bay WA

Participants: Katie Jackson and Jake Heare

We followed a procedure similar to that at Manchester. The difference being that the treatment water was not replaced after each treatment. We also counted the dead in each tray.

1H1-4
Brooders 0
Gaping    49
Dead      7
Closed   33
% Open  59.8%

1N5-8
Brooders 0
Gaping    46
Dead      7
Closed  14
% Open  76.7%

1S13-16
Brooders 0
Gaping     59
Dead        8
Closed    26
% Open  69.4%













5-16-2014

Fidalgo WA

Participants: Steven Roberts and Jake Heare

Same procedure as at Oyster Bay. 

2N1-4
Brood 0
Open 53
Dead  0
Closed  46
% Open  53.5%

2S13-16
Brood 0
Gaping  55
Dead   0
Closed 39
% Open  58.5%

2H5-8
Brood 0
Gaping 48
Dead  0
Closed  52
% Open   48%