Jake Heare Research Central: Oyster Bay

Showing posts with label Oyster Bay. Show all posts

Thursday, June 5, 2014

6 5 2014 oyster bay repro check

Shelton, wa

Participants. Katie Jackson and Jake heare.

High 60s to mid 70s sunny

Followed the anesthesia protocol but modified it by adding ice block and bags of Ice into pools. Temperature started at 13 but stabilized at 16 c. Temp increase unavoidable but non lethal.

Numbers as follow

Temps
Pretreatment.
Initial. 13
45 min. 16
1.5 hrs. 16

Treatment
Initial.    10
45 min    17
1.5 hrs.   16
2.25 hrs.   16

Recovery
Initial 13
45 min. 17
1.5 hrs 17

Salinity
Pretreatment.     26
Treatment.          63
Recovery.            26

Brood collection.
1S13-16
Brood. 2
Gaping. 60
Dead. 12

Brooders
#     size.    Sick
1.     23.        W
2.     29.        W

1H1-4.
Brood.   2
Gaping 79
Dead.     7

Brooders
#      size.      Sick
1.       25.         W
2.       24.          Grey

1N5-8
Brood. 1
Gaping. 52
Dead 7

Brooders
# size. Sick
1. 30. W

Multiple males with milt in their shells. Possibly spawning at the moment.

Thursday, May 29, 2014

5 29 2014 oyster bay repro check

Shelton wa
Mid 60s

Performed standard repro check from anesthesia sop. Found brooders!

Also took temp, salinity and size of brooding females.

Numbers as follow:

Temps
President treatment bath
Initial. 15.5
45 min. 15.5
1.5 hrs. 16.5

Treatment temp
Initial. 11
45 min. 12
1.5 hrs. 15
2.25 hrs. 16

Recovery bath Temps
Initial.   14
45 min   17
1.5 hrs.   18
2.25 hrs. 18

Salinity in ppt
Pretreatment. 25
Treatment. 70
Recovery. 25

Brooding data
1H1-4
Brood. 1
Gaping 80
Dead.    7
Brooders info
#       size (mm).      Larvae taken
1.        24.                   y

1S13-16
Brood.   5
Gaping 74
Dead.     9
Brooders info
#        size (mm)         larvae taken
1.        36.                       Y
2.         26.                      Y
3.         26.                      Y
4.         25.                      Y
5.         28.                      Y

1N5-8
Brood.   2
Gaping 51
Dead.     6
Brooders info
#            size (mm)         larvae taken
1.            27.                     Y
2.            28.                      Y

Brooding larvae are easy to spot. They appear as pools or swarms of white material around the mouth portion of the visceral mass. If oyster has water still in it they maybe swarming inside of the animal. To confirm their presence use black tip of zip tie to collect small portion for viewing. Small white grainy substance is brooding larvae. Grey or green substance most likely algae or food.

Also hazard note. Small portion of ethanol fell on to some gaping animals when transferring larvae to tube. Washed off immediately but worried that undue mortality may occur. That or drunk oyster party.

Attempted to get pics of brooding but not good ones. Posted below.

Friday, May 23, 2014

5 23 2014 oyster bay emergency sampling

Oyster bay, WA

Mid 60s 70s

Participants Steven Roberts, Brent vadopalas, and Jake heare

We came back to oyster bay today to check if mortality occurred across the board.

Upon inspection of the three remaining stacks. There was no indication that the mortality event was spread to the other trays. It seems that a mix of extended exposure, high temperatures, and the treatment caused a mass mortality event. Dabob was the first group to be treated and thus did not experience the same duration of high heat and low oxygen that the north and south sound trays did. We decided to same from the affected trays anyway. We collected 8 from the north sound tray, 4 from the south sound tray, and 10 from the dabob tray. We also individually bagged the shells from the dead oyster in hopes of using them for future data efforts.

On a side note, there appeared to be 2 dabob oysters that had recently spawned as evidenced by the post gonadal canals. Since they showed no signs of brooding it is assumed they spawned as male and may spawn again in the near future as female.

We also did a semi brood check on the other full set of trays and found no brooders.

Shells were placed on dry ice, tissue samples stored in rna later. Will be transferred to cold storage in the near future.

Will continue repro check at fidalgo tomorrow.

Enjoy this selfie of Brent and I in the car as I write reports.

Thursday, May 22, 2014

5 22 2014 oyster bay repro

Oyster bay, WA

High mid 70s.

Participants Katie Jackson and Jake heare.

Tested for reproductive active today. Very bad news.

There has been a huge mortality event at oyster bay. Numbers for brooding, gaping, dead, and alive but not open are as follows.

1n13-16
Brood. 0
Gaping. 3
Dead 93
Alive 5

1s5-8
Brood 0
Gaping 2
Dead 68
Alive 4

1h5-8
Brood 0
Gaping 47
Dead 51
Alive. 9

Clearly dabob has survived whatever mortality event much better than north and south sounds populations. We will be sampling this site tomorrow to ensure fresh samples.

Monday, May 19, 2014

5-14 to 15-16 2014 Reproduction Work Up

5-14-2014

Manchester WA

Participants: Brent Vadopalas and Jake Heare

We retrieved the next set of trays in the dosing sequence and allowed each tray to dessicate for 45 minutes. Trays were then treated in a 10 gallon bath of 50% sea water to 50% freshwater mixed with 7 lbs of epsom salt. The tray was treated for 45 minutes, at which point it was removed and gaping animals were examined for signs of brooding. After examination the trays were then placed into a recovery tub with 100% sea water until the last tray examined had be in the recovery tub for 45 minutes. After each treatment the treatment water was replaced with fresh treatment to reduce temperature flux. Then trays were rebuilt into a stack and hung off the dock.

We counted the number of gaping animals and brooders for each tray.

They are as follows:

4H1-4

brooders 0

Gaping 25

Closed 72
% Open 25.8%

4S9-12

Brooders 0

Gaping 43

Closed 55
% Open 43.9%

4N9-12

Brooders 0

Gaping 31

Closed 28
% Open 52.5%

5-15-2014

Oyster Bay WA

Participants: Katie Jackson and Jake Heare

We followed a procedure similar to that at Manchester. The difference being that the treatment water was not replaced after each treatment. We also counted the dead in each tray.

1H1-4

Brooders 0

Gaping 49

Dead 7

Closed 33
% Open 59.8%

1N5-8

Brooders 0

Gaping 46

Dead 7

Closed 14
% Open 76.7%

1S13-16

Brooders 0

Gaping 59

Dead 8

Closed 26
% Open 69.4%

5-16-2014

Fidalgo WA

Participants: Steven Roberts and Jake Heare

Same procedure as at Oyster Bay.

2N1-4

Brood 0

Open 53

Dead 0

Closed 46
% Open 53.5%

2S13-16

Brood 0

Gaping 55

Dead 0

Closed 39
% Open 58.5%

2H5-8

Brood 0

Gaping 48

Dead 0
Closed 52
% Open 48%

Wednesday, May 7, 2014

Oyster Bay 5-1-14

5-1-14

Shelton WA
Oyster Bay/Totten Inlet
Start Time: 9:30 AM
Finish Time: 5:30 PM
Temps: Mid 60s to Mid 80s

Participants: Jake Heare

I arrived earlier in the morning. I pulled up the hanging devices and broke them down into individual trays. I collected dead, counted live, and photographed trays for size/growth for later analysis. Upon completing these metrics the trays were reorganized into stacks containing one tray from each population at a random level in stack (top, middle, bottom). Since one stack is missing we were unable to completely reorganize the trays as such. One hanging tray has 2 Hood Canal Pops and 1 North Sound pop tray. 2 stacks were then rebuilt and hung off the docks.

The 3rd stack was treated with an epsom salt mixture to anesthetize the animals for brooding larvae inspection. One tray was dosed at a time in a 10 gallon mixture of 50/50 sea water/freshwater with 6-8 cups of epsom salt in them (roughly 75-80 g/L). Gaping oysters in each tray were individually inspected for brooding larvae. Once inspection was completed, trays were then transferred to a tub filled with 10-15 gallons of fresh seawater to recover from treatment for 30 minutes to 1 hour depending on recovery rate. In the first tray we had 25 animals open, in the second we had 3, in the third we had 4. None of these animals showed signs of brooding larvae.