7 18 2014 fidalgo bay repro check

Anacortes, WA

Mid 60s to mid 70s

Participants: alicia Godersky and Jake heare

Performed anesthesia sop with insulation on treatment tub.  Found brooders in only the dabob pop. It seems that they spawn later in the season but in smaller number. We will see in the coming weeks. Also treatment was surprisingly ineffective with no discernable reason. Water was calm. Temps were stable. No movement while in tubs. Full treatment strength and time.  Hopefully it was a fluke.

Numbers as follow:

Temps in c
Pretreatment
Initial.     10
45.         11
1.5.         11

Treatment
Initial.   10
45.       10
1.5.       11
2.25.     11

Recovery
Initial.      10
45.          11
1.5.          12

Salinity in ppt
Pretreatment.       28
Treatment.           66
Recovery.            28

Brood collection

2S1-4
Brood.      0
Gaping.     67
Dead.        0
Closed.     30

2H9-12
Brood.      2
Gaping.    32
Dead.       0
Closed.     50

Brooders
#     size.     Sick
1.       19.        W
2.      20.       W

2N13-16
Brood.     0
Gaping.    68
Dead.       0
Closed.    40

7 17 2014 oyster bay repro

Oyster bay

High 60s to high 70s

Participants:  alicia Godersky and Jake heare

Performed anesthesia sop with insulation on treatment and tarp with ice blocks on recovery tubs. Very heavy mussel set on all trays and oysters. Possibly causing spawning issues. Only one population had brooders which is very different than in previous weeks. The one pop was the dabob pop with 5 brooders, one of which looked like aborted spawn.

Numbers as follow.

Temps in c
Pretreatment
Initial.   16
45.      18
1.5.       18

Treatment
Initial.     13
45.         14
1.5.         14
2.25.       14

Recovery
Initial.      16
45.          16
1.5.          17

Salinity in ppt
Pretreatment.       24
Treatment.           66
Recovery.            25

Brood collection
1S13-16.        
Brood.     0
Gaping.     75
Dead.       1
Closed.     2

1N5-8
Brood.      0
Gaping.     48
Dead.        1
Closed.      1

1H1-4
Brood.      5. 
Gaping.     68
Dead.        6
Closed.      1

Brooders
#     size.    Sick
1.       30.      W
2.       20.     W
3.       26.      W
4.       26.      W
5.       27.      W. Possibly abort spawn.

7 16 2014 Manchester Repro Check

Manchester WA

Low 70s to Mid 80s

Participants: Alicia Godersky and Jake Heare

Performed the anesthesia SOP with insulation and ice blocks on the treatment group and tarp over the recovery and pretreatment tubs. Finally found multiple brooders in one population. If you can guess the pop, you can consider yourself an Oly Whisperer. The brooding animals were also much smaller than expected. Half were less than 20 mm which is quite odd.  Also temps fluxed on the tubs on the dock even with replacing water, the combination of sun and no breeze just baked the docks.

Numbers as follow:

Temps in C
Pretreatment
Initial     10
45         12
1.5        13

Treatment
Initial      12
45          13
1.5         12
2.25       11

Recovery
Initial      12
45          14
1.5         15


Salinity in ppt
Pretreatment      26
Treatment          66
Recovery           25


Brood Collection
4S5-8
Brood    3
Gaping   59
Dead      12
Closed   12

Brooders
#     size     sick
1      22      w
2      16      w
3      20      w

4H13-16
Brood     1
Gaping    56
Dead       8
Closed    17

Brooders
#     size    sick
1      17     W

4N13-16
Brood     0
Gaping    61
Dead       6
Closed    8

7 11 2014 fidalgo repro check

Anacortes, wa

Low 70s to mid 80s

Performed the anesthesia sop with insulation and ice blocks on treatment and tarp cover on Pretreatment and recovery.  Found lots of brooders in southern pop and none in the other two pops. South sound oysters seem to be very attune to tidal changes. Also temps remained within range using the tarp cover. I think I should add a board or stand to keep the tarp higher over the tubs to keep it out of the water and allow for air flow to keep air temps lower. Also note to self, next week I absolutely have to refill my EtOH, 50 ml falcon tubes, and zipties. Also need a new water proof write in the rain pen as I have lost my only good one.

Numbers as follow :

Temps in c
Pretreatment
Initial.   16
45.         16
1.5.        17

Treatment
Initial.          14
45.                15
1.5.               14
2.25.             14

Recovery
Initial.         16
45.               16
1.5.              17

Salinity
Pretreatment.         20
Treatment.              68
Recovery.               23

Brood collection

2H5-8
Brood.     0
Gaping.   70
Dead.       0
Closed.    28

2S13-16
Brood.       11
Gaping.     81
Dead.         0
Closed.      11

Brooders
#    size.    Sick
1.    27.       W
2.    25.       W
3.    26.       W
4.    30.       W
5.    28.       W
6.    30.       W
7.    27.       W
8.    31.       W
9.    25.       W
10.  32.       W
11.  31.       W

2N1-4
Brood.      0
Gaping.    78
Dead.        0
Closed.     17

7 10 2014 oyster bay repro check

Klamiche shores, wa

Hi 60s to low 80s

Performed the anesthesia sop with insulation and ice blocks on treat. I also covered the Pretreatment and recovery tubs with a tarp to shade them from direct sun to help moderate the water temps. It worked well along with adding fresh seawater and leftover ice blocks, keeping the trays within 3 degrees of ambient. Also I broke the one day record for number of brooders. All pops had multiple brooders with the northern pop almost being equal to the southern pop in terms of numbers. Dabob pop also performed very well. I think this has to do with last week's neap tide and the much warmer than average air temps.

Numbers as follow.

Temps in c
Pretreatment
Initial.        16
45.              16
1.5.             16

Treatment
Initial.         14
45.               14
1.5.              13
2.25.            12

Recovery
Initial.          16
45.               17
1.5.               17

Salinity in ppt
Pretreatment.     25
Treatment.          65
Recovery.            24

Brood collection

1N9-12
Brood.      8
Gaping.    73
Dead.        3
Closed.     2

Brooders
#     size.     Sick
1.     30.        W
2.     22.        W  no sample, very few larvae
3.     39.        W
4.     35.        W
5.     31.        W
6.     33.        W
7.     28.        W
8.     32.        W  no sample tile oyster

1H9-12
Brood.       6
Gaping.     83
Dead.         3
Closed.      3

Brooders
#      size.     Sick
1.      27.        W
2.      27.        W.
3.      28.        W
4.      26.        W
5.      26.        W
6.      27.        W

1S1-4
Brood.      10
Gaping.    82
Dead.        0
Closed.     1

Brooders
#        size.       Sick
1.        22.          W
2.        22.          W
3.        25.          W
4.        28.          W
5.        27.          W
6.        27.          W
7.        25.          W
8.        24.          W
9.        30.          W
10.      30.          W.  No sample, tile oyster

7 9 2014 Manchester Repro Check

Manchester, WA

Hi 60s to Mid 80s, sunny

Participants: Joe Stevick and Jake Heare

Used the anesthesia SOP with insulation modification and ice blocks in both the treatment and recovery trays. Treatment stayed within 1 degree, while recovery trays stayed within 5 degrees. Found 1 brooder from the Dabob population which was pretty interesting considering that none of the other populations have been spawning.

Numbers as follow:

Temps in C

Pretreatment
Initial        11
45 min      13
1.5 hr       16


Treatment
Initial       11
45 min     12
1.5 hr      11
2.25 hr    10

Recovery
Initial       13
45 min     16
1.5 hr      17

Salinity in PPT

Pretreatment     26
Treatment        53
Recovery         27


Brood Collection
4N9-12
Brood       0
Gaping      45
Dead        12
Closed      2

4S9-12
Brood       0
Gaping      55
Dead        22
Closed      15

4H1-4
Brood       1
Gaping      60
Dead         5
Closed      21

Brooders
#       size     sick
1       21       w

7 4 2014 Fidalgo repro check

Anacortes wa

Mid 60s to low 70s

Participants: Jim cerkovnik and Jake heare

Performed anesthesia sop with insulation on treatment tub. Found brood Dr s in all three pops. Majority in southern pop, equal numbers in northern and dabob.

Numbers as follow:

Temps in c
Pretreatment
Initial.        9
45              11
1.5.            12

Treatment
Initial.        12
45.              12
1.5.             12
2.25.           12

Recovery
Initial.          13
45.                13
1.5.               15

Salinity in ppt
Pretreatment.       31
Treatment.            65
Recovery.              32

Brood collection
2H13-16
Brood.     1
Gaping.   88
Dead.       0
Closed.    9

Brooders
#         size.     Sick
1.         23.         W

2S9-12
Brood.      6
Gaping     71
Dead.        0
Closed.     14

Brooders
#     size.     Sick
1.     30.        W
2.     32.         W
3.     32         W
4.      41.        W
5.       32.      Grey
6.       21.       W

2N5-8
Brood.      1
Gaping.     82
Dead.        0
Closed.      13

Brooders
#     size.     Sick
1.       28.      W
 

7 3 2014 oyster bay repro check

Shelton, wa

Mid 60s to high 70s cloudy mostly till 2 pm then sunny

Performed the anesthesia sop with insulation on treatment. No ice blocks needed. Found no brooders in North pop but 3 in dabob. Lots still from south sound pop.

Numbers as follow

Temps in c
Pretreatment
Initial.      15
45.            15
1.5.           16

Treatment
Initial.       11
45.             10
1.5.            11
2.25.          11

Recovery
Initial.     16
45.           19
1.5.           22 sun came out. Kept adding water.

Salinity in ppt
Pretreatment.       25
Treatment.            73
Recovery.              26

Brood collection
1N5-8
Brood.    0
Gaping.   49
Dead.      0
Closed.    1

1H1-4
Brood.       3
Gaping.     66
Dead.         4
Closed.      4

Brooders
#          size.      Sick
1.          25.         W
2.          26.          Grey
3.          25.          W

1S13-16
Brood.     9
Gaping.   78
Dead.      0
Closed.    3

Brooders
#       size.        Sick
1.       26.           W
2.       28.           W
3.       26.           W
4.       30.           W
5.       39.           W
6.       25.           W
7.       28.           W
8.       29.           W
9.       25.           W. No larvae collected from 9. Tile oyster.

7 2 2014 Manchester Repro Update

Manchester WA

Mid 60's to High 70s Sunny

Participants: L. Christine Savolainen and Jake Heare

Used the Anesthesia SOP with the insulation and ice blocks on the treatment tubs. Found absolutely no brooders. This site just does not have the conditions necessary for these animals to spawn. It must be due to the colder deep water that comes into the bay off the Sound.

Numbers as Follow:

Temps in C

Pretreatment
Initial     10
45 min   11
1.5 hr     15 * sun came out

Treatment
Initial     11
45 min   11
1.5 hr    10
2.25       9

Recovery
Initial     11
45 min   13
1.5 hr    15

Salinity in ppt
Pretreatment    28
Treatment        67
Recovery        28

Brood Collection

4H9-12
Brood          0
Gaping         52
Dead           20
Closed         8

4S13-16
Brood           0
Gaping          31
Dead             4
Closed          48 *Lots of boat traffic shaking the dock

4N1-4
Brood          0
Gaping        40
Dead           20
Closed         18

6 27 2014 fidalgo repro check

Anacortes wa

Mid 50 to mid 60s

Participants:  Sean Bennett and Jake heare

Followed the anesthesia sop modified with insulation on bottom of tub. Too windy to put cover on, also forgot to get ice. Cool enough to keep treatment with 3 degrees. Found brooders in south sound and north sound pop but none in dabob still.

Numbers as follow:

Temps in c

Pretreatment
Initial.         9
45.              10
1.5.             10

Treatment
Initial.            9
45.                 10
1.5.                10
2.25.              12

Recovery
Initial.          10
45.                10
1.5.               14

Salinity in ppt
Pretreatment.           29
Treatment.                58
Recovery.                 28

Brood collection

2S5-8
Brood.          3
Gaping.        87
Dead.            0
Closed.        6

Brooders
#         size.         Sick
1.         27.             W
2.         25.             W
3.         26.             W

2H1-4
Brood.       0
Gaping.     65
Dead.         0
Closed.      28

2N9-12
Brood.      1
Gaping.    87
Dead.        0
Closed.     6

Brooders
#         size.         Sick
1.         31.            W

6 26 2014 oyster bay repro

Used the modified anesthesia sop with insulation and ice blocks. Collected from the water the lost stack as well as the hhn stack to reshuffle oyster trays. Big things of note, 1 south sound tray was 99% dead. It was a middle tray, had no predators and no fouling. No idea why those animals died but the other two trays in the stack survived. Brooding in all sampled trays seemed to have followed expectations. With no pop with way more or way less brooders than expected. Also collected temp logger info. All trays were kept in ambient seawater during Pretreatment or out plant if not sampled

Lost trays marked with orange ziptie, hhn trays labeled with blue ziptie.

Numbers as follows:

Temps in c

Pretreatment
Initial.       14
45.             14
1.5.            14
2.25.          16

Treatment
Initial.        11
45.              11
1.5.             11
2.25.            9
3.                 8

Recovery
Initial.          13
45.                15
1.5.               16
2.25.             16

Salinity
Pretreatment.         26
Treatment.               68
Recovery.                 24

Trays not used for Brood collection

1S9-12
Live.           1
Dead.         82

1H13-16
Live.          12
Dead.         0

Brood collection

1N1-4. From hhn stack
Brood.       2
Gaping.     72
Dead.         10
Closed.      1

Brooders
#        size.      Sick
1.        25.         W
2.        31.         Grey

(Three previous trays were put out immediately as stack 1. Far left from shore)

1H9-12
Brood.       1
Gaping.      92
Dead.         4
Closed.      1

Brooders
#      size.      Sick
1.      27.          Grey

1S1-4
Brood.       11
Gaping.      85
Dead.          10
Closed.       2

Brooders
#          size.         Sick
1.          29.            W
2.          25.            W
3.          28.            W
4.          30.            W
5.          26.            W
6.          30.            W
7.          25.            W
8.          26.            W
9.          28.            W
10.        31.            W
11.        27.            W

10/11. Were dirty samples, had lots of mud mixed in with larvae.

1N9-12
Brood.          5
Gaping.        84
Dead.            16
Closed.          1

Brooders
#        size.        Sick
1.        31.           W
2.        29.           W
3.        36.           W
4.        28.           W
5.        30.           W

6 25 2014 Manchester Repro Check

Manchester WA

Mid 60's to mid 70s sunny

Participants: L. Christine Savolainen and Jake Heare

Performed the Anesthesia SOP modified with insulation on the treatment tub as well as ice blocks to keep temperatures within range. Found one brooder in the Northern population with minimal larvae and found one brooder from Dabob that had a very strange mix of eggs and what appeared to be semen. This might be evidence for an abortive spawn. also collected all mortalities from trays.

numbers as follow.

Temps in C

Pretreatment
Initial       10
45 min     15
1.5 hr       15

Treatment
Initial       11
45 min     11
1.5 hrs     11
2.25 hr     11

Recovery
Initial        10
45 min      15
1.5 hr        17


Salinity
Pretreatment      27
Treatment          65
Recovery           21


Brood Collection

4S1-4
Brood    0
Gaping     50
Dead      22
Closed    13

4N5-8
Brood        1
Gaping       53
Dead         23
Closed       19

Brooders
#       size      sick
1        25        w

4H5-8
Brood       1
Gaping      52
Dead        7
Closed      33

Brooders
#        size         sick
1        15           W

Harbor Seal watching us work all day. 

6 20 2014 fidalgo repro check

Anacortes wa

Mid 60s cloudy to sunny

Participants: L. Christine savolainen and Jake heare

Followed the anesthesia sop with insulation on the treatment group.  Found one brooder from the southern population. Two animals looked like possible brooders in the northern pop but they closed quickly and I was unable to confirm brooding in them.

Also Brian found the missing stack at oyster bay. They will be worked up next Thursday as they are safely secured to the dock again.

Numbers as follows

Temps in c
Pretreatment.
Initial.       8
45 min.     8
1.5 hrs.     9

Treatment
Initial.           9
45 min.         9
1.5 hrs.         10
2.25 hrs.       10

Recovery
Initial.        7
45 min.      8
1.5 hrs.      8

Salinity in ppt
Pretreatment.      35
Treatment.           65
Recovery.             35

Brood collection
2S1-4
Brood.       1
Gaping.     83
Dead.         0
Closed.      10
Brooders
#       size.     Sick
1.       27.        W

2H9-12
Brood.        0
Gaping.      77
Dead.          0
Closed.       10

2N13-16
Brood.     0
Gaping.    76
Dead.       0
Closed.    34