20120208 - cDNA Calcs - Google Sheets

	A	B	C	D	E
1	Sample ID	ng/ul	Vol. for 175ng (uL)	Random Primers for 4.375ng (uL)	H2O to 18.25uL
2	DNased RNA 4-12-11 #132	15.06	11.62	0.875	5.75
3	DNased RNA 4-12-11 #134	17.05	10.26	0.875	7.11
4	DNased RNA 4-12-11 #136	12.27	14.26	0.875	3.11
5	DNased RNA 4-12-11 #138	12.51	13.99	0.875	3.39
6	DNased RNA 4-12-11 #140	13.54	12.92	0.875	4.45
7	DNased RNA 4-12-11 #142	10.21	17.14	0.875	0.23
8	DNased RNA 4-12-11 #407	12.95	13.51	0.875	3.86
9	DNased RNA 4-12-11 #409	11.44	15.30	0.875	2.08
10	DNased RNA 4-12-11 #411	12.07	14.50	0.875	2.88
11	DNased RNA 4-12-11 #413	11.68	14.98	0.875	2.39
12	DNased RNA 4-12-11 #415	12.93	13.53	0.875	3.84
13	DNased RNA 4-12-11 #417	11.54	15.16	0.875	2.21
14
15	Master Mix	Single (uL)	# Rxns	Total (uL)
16	5x Buffer	5	13	65
17	10mM dNTPs	1.25	13	16.25
18	MMLV RT	0.0875	13	1.1375
19	Total			Add 6.75uL to each tube