20150616_Jake_Oly_cDNA_Calcs

	A	B	C	D	E	F	G	H	I	J	K	L	M
1	Plate Position	Sample ID	[DNased RNA] (ng/ul)	uL to 250ng RNA	uL dT primers (0.5ug/ug RNA) of a 1:10 dilution	H2O to 74.75uL	5x rxn buffer (uL)	10mM dNTPs (uL)	MMLV (uL)	Master mix	Single Reaction (uL)	# Reactions	Total (uL)

2	A07	DNased 42215 HC1	81.16	3.08	1.25	70.42	20	5	0.25	5x buffer	20	53	1060
3	B07	DNased 42215 HC2	79.93	3.13	1.25	70.37	20	5	0.25	dNTPs	5	53	265
4	C07	DNased 42215 HC3	79.08	3.16	1.25	70.34	20	5	0.25	MMLV	0.25	53	13.25
5	D07	DNased 42215 HC4	79	3.16	1.25	70.34	20	5	0.25
6	E07	DNased 42215 HC5	82.94	3.01	1.25	70.49	20	5	0.25
7	F07	DNased 42215 HC6	80.97	3.09	1.25	70.41	20	5	0.25
8	G07	DNased 42215 HC7	84.08	2.97	1.25	70.53	20	5	0.25
9	H07	DNased 42215 HC8	79.94	3.13	1.25	70.37	20	5	0.25
10	A08	DNased 42215 NC1	79.2	3.16	1.25	70.34	20	5	0.25
11	B08	DNased 42215 NC2	91.37	2.74	1.25	70.76	20	5	0.25
12	C08	DNased 42215 NC3	76.32	3.28	1.25	70.22	20	5	0.25
13	D08	DNased 42215 NC4	83.31	3.00	1.25	70.50	20	5	0.25
14	E08	DNased 42215 NC5	83.75	2.99	1.25	70.51	20	5	0.25
15	F08	DNased 42215 NC6	85.91	2.91	1.25	70.59	20	5	0.25
16	G08	DNased 42215 NC7	82.1	3.05	1.25	70.45	20	5	0.25
17	H08	DNased 42215 NC8	74.52	3.35	1.25	70.15	20	5	0.25
18	A09	DNased 42215 SC1	78.11	3.20	1.25	70.30	20	5	0.25
19	B09	DNased 42215 SC2	84.15	2.97	1.25	70.53	20	5	0.25
20	C09	DNased 42215 SC3	79.46	3.15	1.25	70.35	20	5	0.25
21	D09	DNased 42215 SC4	84.98	2.94	1.25	70.56	20	5	0.25
22	E09	DNased 42215 SC5	81.99	3.05	1.25	70.45	20	5	0.25
23	F09	DNased 42215 SC6	70.29	3.56	1.25	69.94	20	5	0.25
24	G09	DNased 42215 SC7	77.08	3.24	1.25	70.26	20	5	0.25
25	H09	DNased 42215 SC8	84.22	2.97	1.25	70.53	20	5	0.25
26	A10	DNased 42215 HT1 1	24.29	10.29	1.25	63.21	20	5	0.25
27	B10	DNased 42215 HT1 2	25.13	9.95	1.25	63.55	20	5	0.25
28	C10	DNased 42215 HT1 3	24.2	10.33	1.25	63.17	20	5	0.25
29	D10	DNased 42215 HT1 4	23.45	10.66	1.25	62.84	20	5	0.25
30	E10	DNased 42215 HT1 5	24.42	10.24	1.25	63.26	20	5	0.25
31	F10	DNased 42215 HT1 6	23.85	10.48	1.25	63.02	20	5	0.25
32	G10	DNased 42215 HT1 7	31.59	7.91	1.25	65.59	20	5	0.25
33	H10	DNased 42215 HT1 8	119.54	2.09	1.25	71.41	20	5	0.25
34	A11	DNased 42215 NT1 1	35.18	7.11	1.25	66.39	20	5	0.25
35	B11	DNased 42215 NT1 2	25.27	9.89	1.25	63.61	20	5	0.25
36	C11	DNased 42215 NT1 3	23.02	10.86	1.25	62.64	20	5	0.25
37	D11	DNased 42215 NT1 4	24.21	10.33	1.25	63.17	20	5	0.25
38	E11	DNased 42215 NT1 5	23.41	10.68	1.25	62.82	20	5	0.25
39	F11	DNased 42215 NT1 6	24.63	10.15	1.25	63.35	20	5	0.25
40	G11	DNased 42215 NT1 7	18.15	13.77	1.25	59.73	20	5	0.25
41	H11	DNased 42215 NT1 8	7.13	35.06	1.25	38.44	20	5	0.25
42	A12	DNased 42215 ST1 1	22.91	10.91	1.25	62.59	20	5	0.25
43	B12	DNased 42215 ST1 2	22.57	11.08	1.25	62.42	20	5	0.25
44	C12	DNased 42215 ST1 3	24.18	10.34	1.25	63.16	20	5	0.25
45	D12	DNased 42215 ST1 4	28.65	8.73	1.25	64.77	20	5	0.25
46	E12	DNased 42215 ST1 5	26.28	9.51	1.25	63.99	20	5	0.25
47	F12	DNased 42215 ST1 6	19.98	12.51	1.25	60.99	20	5	0.25
48	G12	DNased 42215 ST1 7	24.4	10.25	1.25	63.25	20	5	0.25
49	H12	DNased 42215 ST1 8	23.26	10.75	1.25	62.75	20	5	0.25