20181017_Cgigas_ploidy_cDNA_calcs

	A	B	C	D	E	F	G	H	I	J	K
1	sample_ID	Dnased_RNA(ng/uL)	vol_to_100ng(uL)	vol_dT_primers_1:10_dilution(uL)	primer_denature_vol(uL)	vol_h2o_to_15uL(uL)	vol_5x_rxn_buffer(uL)	vol_10mM_dNTPs(uL)	vol_MMLV(uL)	vol_h2o_to_25uL(uL)	vol_final(uL)
2	Dnased_D01-C	25.1	3.98	1	15	7.03	5	1.25	0.1	3.65	25
3	Dnased_D02-C	20.3	4.93	1	15	5.15	5	1.25	0.1	3.65	25
4	Dnased_D09-C	23.8	4.20	1	15	6.60	5	1.25	0.1	3.65	25
5	Dnased_D10-C	21.9	4.57	1	15	5.87	5	1.25	0.1	3.65	25
6	Dnased_D11-C	20.1	4.98	1	15	5.05	5	1.25	0.1	3.65	25
7	Dnased_D12-C	27.3	3.66	1	15	7.67	5	1.25	0.1	3.65	25
8	Dnased_D19-C	13.9	7.19	1	15	0.61	5	1.25	0.1	3.65	25
9	Dnased_D20-C	25.2	3.97	1	15	7.06	5	1.25	0.1	3.65	25
10	Dnased_T01-C	22.4	4.46	1	15	6.07	5	1.25	0.1	3.65	25
11	Dnased_T02-C	21.4	4.67	1	15	5.65	5	1.25	0.1	3.65	25
12	Dnased_T09-C	25.2	3.97	1	15	7.06	5	1.25	0.1	3.65	25
13	Dnased_T10-C	19.9	5.03	1	15	4.95	5	1.25	0.1	3.65	25
14	Dnased_T11-C	20.6	4.85	1	15	5.29	5	1.25	0.1	3.65	25
15	Dnased_T12-C	18.2	5.49	1	15	4.01	5	1.25	0.1	3.65	25
16	Dnased_T19-C	25.9	3.86	1	15	7.28	5	1.25	0.1	3.65	25
17	Dnased_T20-C	26.5	3.77	1	15	7.45	5	1.25	0.1	3.65	25
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21	MMLV_master_mix	vol_single rxn	number of reactions	vol_total(uL)
22	5x_rxn_buffer	5	18	90
23	10mM_dNTPs	1.25	18	22.5
24	H2O	3.65	18	65.7
25	MMLV	0.1	18	1.8
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27				Add 10uL to each sample.
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