20181018_qPCR_Cgigas_ploidy_cDNA - Google Sheets

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	A	B	C	D	E	F	G	H	I	J	K	L	M	N	O	P	Q	R	S	T
1	Master Mix Calcs
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4		Ci	Cf	Vol. (uL)	# Rxns	Total Vol (uL)
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6	Template (cDNA)			1
7	2x Sso Fast EVAgreen	2	1	10	40	400
8	Pf	10	0.2	0.5	40	20
9	Pr	10	0.2	0.5	40	20
10	H2O			8	40	320
11	Total			20		Add 19.0uL to each well
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