A.cervicornis_DNA_Extractions(May_2017).xlsx

	A	B	C	D	E	F	G	H	I	J	K	L	M	N	O	P	Q	R	S	T	U	V
1	Tube	Sample ID	[DNA] ng/ul (NanoDrop)	[DNA] ng/uL (qubit)	Qubit Date	Qubit Initials	DNA in 8uL	Vol for 77.12ng DNA	Volume of 1:10 dilution	H2O to 8uL	Target Conc	Additional DNA	Additional H2O	Total DNA	Final Vol	Methylation Plate Position	Plate	Abs_01(450nm)	Abs_02(450nm)	Mean Abs	Methylated DNA(ng)	Percent Methylated

2	1	2h Block 1	175.5	9.64	20170510	SJW	77.12	8.00	NO DILUTION	0.00	9.64	9.60	0.00	17.60	17.60	G01	1	0.449		0.449	2.96	3.84
3	2	2h Block 2	126.5	16.6	20170510	SJW	132.80	4.65	NO DILUTION	3.35		5.57	4.03	10.22	17.60	G02	1	0.100	0.195	0.147	0.62	0.81
4	3	2h Block 3	420.5	63.8	20170510	SJW	510.40	1.21	NO DILUTION	6.79		1.45	8.15	2.66	17.60	A03	1	0.206	0.168	0.187	0.93	1.21
5	4	2h Block 4	443.5	53.8	20170510	SJW	430.40	1.43	NO DILUTION	6.57		1.72	7.88	3.15	17.60	C03	1	0.122	0.109	0.116	0.38	0.49
6	5	2h Block 5	266.0	26.2	20170510	SJW	209.60	2.94	NO DILUTION	5.06		3.53	6.07	6.48	17.60	E03	1	0.103	0.138	0.120	0.41	0.54
7	6	2h Block 6	259.5	37.4	20170510	SJW	299.20	2.06	NO DILUTION	5.94		2.47	7.13	4.54	17.60	G03	1	0.107	0.197	0.152	0.66	0.86
8	7	2h Block 8	187.0	21	20170511	SJW	168.00	3.67	NO DILUTION	4.33		4.41	5.19	8.08	17.60	A04	1	0.328	0.248	0.288	1.71	2.22
9	8	2h Block 9	156.5	13.4	20170510	SJW	107.20	5.76	NO DILUTION	2.24		6.91	2.69	12.66	17.60	C04	1	0.159	0.110	0.135	0.53	0.68
10	9	2h Block 10	153.5	16	20170510	SJW	128.00	4.82	NO DILUTION	3.18		5.78	3.82	10.60	17.60	E04	1	0.155	0.133	0.144	0.60	0.78
11	10	72h Block 1	194.5	19.4	20170510	SJW	155.20	3.98	NO DILUTION	4.02		4.77	4.83	8.75	17.60	G04	1	0.207	0.239	0.223	1.21	1.56
12	11	72h Block 2	239.5	26.6	20170510	SJW	212.80	2.90	NO DILUTION	5.10		3.48	6.12	6.38	17.60	A05	1	0.215	0.175	0.195	0.99	1.29
13	12	72h Block 3	198.5	25	20170510	SJW	200.00	3.08	NO DILUTION	4.92		3.70	5.90	6.79	17.60	C05	1	0.105	0.086	0.095	0.22	0.28
14	13	72h Block 4	2765.0	187	20170510	SJW	1,496.00	0.41	4.12	3.88		4.95	4.65	9.07	17.60	E05	1	0.083	0.114	0.098	0.24	0.32
15	14	72h Block 5	436.4	48	20170510	SJW	384.00	1.61	NO DILUTION	6.39		1.93	7.67	3.53	17.60	G05	1	0.121	0.179	0.150	0.64	0.83
16	15	72h Block 6	438.0	49.8	20170510	SJW	398.40	1.55	NO DILUTION	6.45		1.86	7.74	3.41	17.60	A06	1	0.169	0.155	0.162	0.74	0.96
17	16	72h Block 8	491.5	44.8	20170510	SJW	358.40	1.72	NO DILUTION	6.28		2.07	7.53	3.79	17.60	C06	1	0.142	0.086	0.114	0.36	0.47
18	17	72h Block 9	120.5	20.2	20170510	SJW	161.60	3.82	NO DILUTION	4.18		4.58	5.02	8.40	17.60	E06	1	0.166	0.141	0.154	0.67	0.87
19	18	72h Block 10	329.5	48	20170510	SJW	384.00	1.61	NO DILUTION	6.39		1.93	7.67	3.53	17.60	G06	1	0.140	0.143	0.142	0.58	0.75
20	19	D7 Block 1	264.5	36.4	20170510	SJW	291.20	2.12	NO DILUTION	5.88		2.54	7.06	4.66	17.60	A07	1	0.114	0.106	0.110	0.34	0.43
21	20	D7 Block 2	320.5	25.4	20170510	SJW	203.20	3.04	NO DILUTION	4.96		3.64	5.96	6.68	17.60	C07	1	0.108	0.086	0.097	0.23	0.30
22	21	D7 Block 3	373.0	43.8	20170510	SJW	350.40	1.76	NO DILUTION	6.24		2.11	7.49	3.87	17.60	E07	1	0.122	0.106	0.114	0.36	0.47
23	22	D7 Block 4	196.0	34.8	20170510	SJW	278.40	2.22	NO DILUTION	5.78		2.66	6.94	4.88	17.60	G07	1	0.210	0.253	0.232	1.28	1.65
24	23	D7 Block 5	237.5	29.4	20170510	SJW	235.20	2.62	NO DILUTION	5.38		3.15	6.45	5.77	17.60	A08	1	0.161	0.129	0.145	0.61	0.79
25	24	D7 Block 6	267.5	34.6	20170510	SJW	276.80	2.23	NO DILUTION	5.77		2.67	6.93	4.90	17.60	C08	1	0.104	0.078	0.091	0.19	0.24
26	25	D7 Block 8	144.5	19.6	20170510	SJW	156.80	3.93	NO DILUTION	4.07		4.72	4.88	8.66	17.60	E08	1	0.123	0.115	0.119	0.40	0.52
27	26	D7 Block 9	173.5	18.4	20170510	SJW	147.20	4.19	NO DILUTION	3.81		5.03	4.57	9.22	17.60	G08	1	0.166	0.185	0.176	0.84	1.09
28	27	D7 Block 10	148.5	27.6	20170510	SJW	220.80	2.79	NO DILUTION	5.21		3.35	6.25	6.15	17.60	A09	1	0.173	0.092	0.132	0.51	0.66
29	28	D14 Block 1	948.0	197	20170510	SJW	1,576.00	0.39	3.91	4.09		4.70	4.90	8.61	17.60	C09	1	0.115	0.117	0.116	0.38	0.50
30	29	D14 Block 2	2748.0	175	20170510	SJW	1,400.00	0.44	4.41	3.59		5.29	4.31	9.70	17.60	E09	1	0.109	0.115	0.112	0.35	0.45
31	30	D14 Block 3	1272.0	193	20170510	SJW	1,544.00	0.40	4.00	4.00		4.80	4.80	8.79	17.60	G09	1	0.132	0.167	0.150	0.64	0.83
32	31	D14 Block 4	642.0	185	20170510	SJW	1,480.00	0.42	4.17	3.83		5.00	4.60	9.17	17.60	A10	1	0.181	0.142	0.161	0.73	0.95
33	32	D14 Block 5	511.0	115	20170510	SJW	920.00	0.67	6.71	1.29		8.05	1.55	14.75	17.60	C10	1	0.123	0.119	0.121	0.42	0.54
34	33	D14 Block 6	1434.0	153	20170510	SJW	1,224.00	0.50	5.04	2.96		6.05	3.55	11.09	17.60	E10	1	0.098	0.111	0.104	0.29	0.37
35	34	D14 Block 8	737.5	147	20170510	SJW	1,176.00	0.52	5.25	2.75		6.30	3.30	11.54	17.60	G10	1	0.160	0.216	0.188	0.94	1.22
36	35	D14 Block 9	909.5	88.8	20170510	SJW	710.40	0.87	NO DILUTION	7.13		1.04	8.56	1.91	17.60	A11	1	0.194	0.131	0.162	0.74	0.96
37	36	D14 Block 10	448.0	102	20170510	SJW	816.00	0.76	7.56	0.44		9.07	0.53	16.63	17.60	C11	1	0.126	0.118	0.122	0.43	0.56
38	37	D20 Block 1	282.0	44.8	20170510	SJW	358.40	1.72	NO DILUTION	6.28		2.07	7.53	3.79	17.60	E11	1	0.106	0.109	0.107	0.31	0.41
39	38	D20 Block 2	152.5	38.4	20170510	SJW	307.20	2.01	NO DILUTION	5.99		2.41	7.19	4.42	17.60	G11	1	0.146	0.198	0.172	0.82	1.06
40	39	D20 Block 3	291.0	59.2	20170510	SJW	473.60	1.30	NO DILUTION	6.70		1.56	8.04	2.87	17.60	A12	1	0.216	0.143	0.180	0.87	1.13
41	40	D20 Block 4	298.0	37.2	20170510	SJW	297.60	2.07	NO DILUTION	5.93		2.49	7.11	4.56	17.60	C12	1	0.134	0.130	0.132	0.50	0.65
42	41	D20 Block 5	201.5	35.6	20170510	SJW	284.80	2.17	NO DILUTION	5.83		2.60	7.00	4.77	17.60	E12	1	0.121	0.156	0.139	0.55	0.72
43	42	D20 Block 6	224.0	73	20170510	SJW	584.00	1.06	NO DILUTION	6.94		1.27	8.33	2.32	17.60	G12	1	0.150	0.171	0.161	0.73	0.94
44	43	D20 Block 8	123.0	35.8	20170510	SJW	286.40	2.15	NO DILUTION	5.85		2.59	7.01	4.74	17.60	G01	2	0.259	0.328	0.294	2.04	2.65
45	44	D20 Block 9	121.5	26.6	20170510	SJW	212.80	2.90	NO DILUTION	5.10		3.48	6.12	6.38	17.60	G02	2	0.238	0.158	0.198	1.18	1.53
46	45	D20 Block 10	115.5	20.8	20170510	SJW	166.40	3.71	NO DILUTION	4.29		4.45	5.15	8.16	17.60	A03	2	0.097	0.123	0.110	0.39	0.51
47	46	D27 Block 1	166.5	28.2	20170510	SJW	225.60	2.73	NO DILUTION	5.27		3.28	6.32	6.02	17.60	C03	2	0.140	0.147	0.144	0.70	0.90
48	47	D27 Block 2	186.5	32.4	20170510	SJW	259.20	2.38	NO DILUTION	5.62		2.86	6.74	5.24	17.60	E03	2	0.481	0.403	0.442	3.38	4.38
49	48	D27 Block 3	202.0	45.4	20170510	SJW	363.20	1.70	NO DILUTION	6.30		2.04	7.56	3.74	17.60	G03	2	0.183	0.221	0.202	1.22	1.58
50	49	D27 Block 4	115.0	35.2	20170510	SJW	281.60	2.19	NO DILUTION	5.81		2.63	6.97	4.82	17.60	A04	2	0.169	0.141	0.155	0.80	1.04
51	50	D27 Block 5	178.0	47.6	20170510	SJW	380.80	1.62	NO DILUTION	6.38		1.94	7.66	3.56	17.60	C04	2	0.145	0.119	0.132	0.59	0.77
52	51	D27 Block 6	358.0	59.4	20170510	SJW	475.20	1.30	NO DILUTION	6.70		1.56	8.04	2.86	17.60	E04	2	0.147	0.123	0.135	0.62	0.80
53	52	D27 Block 8	83.0	20.2	20170510	SJW	161.60	3.82	NO DILUTION	4.18		4.58	5.02	8.40	17.60	G04	2	0.789	0.798	0.793	6.53	8.47
54	53	D27 Block 9	114.5	39	20170510	SJW	312.00	1.98	NO DILUTION	6.02		2.37	7.23	4.35	17.60	A05	2	0.151	0.227	0.189	1.10	1.43
55	54	D27 Block 10	127.0	17.8	20170510	SJW	142.40	4.33	NO DILUTION	3.67		5.20	4.40	9.53	17.60	C05	2	0.141	0.175	0.158	0.83	1.07
56	55	D35 Block 1	155.5	15.3	20170510	SJW	122.40	5.04	NO DILUTION	2.96		6.05	3.55	11.09	17.60	E05	2	0.125	0.139	0.132	0.59	0.77
57	56	D35 Block 2	163.5	19.9	20170510	SJW	159.20	3.88	NO DILUTION	4.12		4.65	4.95	8.53	17.60	G05	2	0.099	0.134	0.116	0.45	0.59
58	57	D35 Block 3	122.5	26.8	20170510	SJW	214.40	2.88	NO DILUTION	5.12		3.45	6.15	6.33	17.60	A06	2	0.113	0.102	0.108	0.37	0.48
59	58	D35 Block 4	149.5	20.8	20170510	SJW	166.40	3.71	NO DILUTION	4.29		4.45	5.15	8.16	17.60	C06	2	0.114	0.147	0.131	0.58	0.75
60	59	D35 Block 5	216.0	44.8	20170510	SJW	358.40	1.72	NO DILUTION	6.28		2.07	7.53	3.79	17.60	E06	2	0.128	0.170	0.149	0.75	0.97
61	60	D35 Block 6	169.0	33	20170510	SJW	264.00	2.34	NO DILUTION	5.66		2.80	6.80	5.14	17.60	G06	2	0.133	0.130	0.132	0.59	0.77
62	61	D35 Block 8	65.0	20.6	20170511	SJW	164.80	3.74	NO DILUTION	4.26		4.49	5.11	8.24	17.60	A07	2	0.340	0.212	0.276	1.89	2.45
63	62	D35 Block 9	636.0	19.3	20170510	SJW	154.40	4.00	NO DILUTION	4.00		4.80	4.80	8.79	17.60	C07	2	0.088	0.127	0.108	0.38	0.49
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