02142020-crab-qcr-samples

	A	B	C	D	E	F	G	H	I	J	K	L	S
1	POSITION	CONTENTS	tube_number	temp	cPCR_infection_status	RNA_ng.ul	sq_all.runs_mean	estimated_vol	estimated_total-RNA_ng	DNAsed	some-to-genewiz	notes	shellfishRNA-box-location

2	A1	6203_113_9	113	c	uninfected	38.1	3070			yes	yes
3	A2	6141_249_12	249	c	infected	18.6	6789	5	93	no	no	aliquoted out for genewiz in 0.5 ul; not sent
4	A3	6101_206_12	206	c	uninfected	19.9	42			yes	no
5	A4	6110_331_12	331	a	uninfected	21.9	89			yes	no		box 7 a 7
6	A5	6155_358_12	358	a	infected	3.71	958	45	166.95	no	no	low RNA concentration, but high elution volume
7	A6	6255_262_12	262	w	infected	41.3	1785			no	no
8	A7
9	A8
10	A9	6141_123_9	123	c	infected	13.1	6789			yes	no	original tube from RNA extraction
11	B1	6203_222_12	222	c	uninfected	30.6	3070			yes	yes
12	B2	6141_451_26	451	c	infected	65.6	6789	5	328	?	no	aliquoted out for genewiz in 0.5 ul; not sent; DNAse unknown because I don't know if aliquot came from 451-1(Grace extracted) or 451 (SJW extracted)
13	B3	6149_226_12	226	c	infected	26.2	66053			no	no
14	B4	6179_315_12	315	a	uninfected	17.8	24			no	no
15	B5	6111_356_12	356	a	infected	21.3	680			yes	no	high RNA concentration, but low hemato qpcr starting quant comapred to other infected samples	box 7 b5
16	B6	6261_273_12	273	w	infected	36.1	3345	15		no	no
17	B7
18	B8
19	B9	6141_249_12	249	c	infected	18.6	6789			no	no	original tube from RNA extraction
20	C1	6203_425_26	425	c	uninfected	29.9	3070			yes	yes
21	C2	6144_14_9	14	a	infected	4.92	1067	5	24.6	no	no	aliquoted out for genewiz in 0.5 ul; not sent
22	C3	6128_231_12	231	c	infected	37.9	7774			no	no
23	C4	6213_310_12	310	a	uninfected	27.3	75			no	no
24	C5	6223_270_12	270	w	uninfected	29.3	0			no	no	not sure i trust qpcr hemato starting quant...
25	C6	6240_362_12	362	w	infected	13.4	2115	15		no	no
26	C7
27	C8
28	C9	6141_451_26	451-2	c	infected	65.6	6789			no	no	original tube from RNA extraction
29	D1	6211_118_9	118	a	infected	51	105477			yes	yes
30	D2	6144_348_12	348	a	infected	5.76	1067	5	28.8	no	no	aliquoted out for genewiz in 0.5 ul; not sent
31	D3	6208_229_12	229	c	infected	2.12	87233	45	95.4	no	no	low concentration, so adding several option for cold, infected group. need to select best 5
32	D4	6123_345_12	345	a	uninfected	19.1	102			yes	no	high qpcr starting quant compared to other uninfected, but high RNA concentration	box7b1
33	D5	6228_290_12	290	w	uninfected	28.5	79			no	no
34	D6	6244_284_12	284	w	infected	27.6	235	15		no	no	low hemat qpcr starting quant
35	D7
36	D8
37	D9	6141_451_26	451	c	infected	4.56	6789			yes	no	original tube from RNA extraction
38	E1	6211_349_12	349	a	infected	5.56	105477			no	yes
39	E2	6144_477_26	477	a	infected	1.84	1067	20	36.8	no	no	aliquoted out for genewiz in 0.5 ul; not sent
40	E3	6164_257_12	257	c	infected	27.2	889			no	no	low qPCR start quant of Hematodinium compared to other infected samples but high RNA concentration.
41	E4	6272_338_12	338	a	uninfected	10.6	2			yes	no	low qpcr starting quant, and lower rna concentration	box 7 g4
42	E5	6230_292_12	292	w	uninfected	17.2	5			no	no
43	E6	6269_364_12	364	w	infected	21.1	257	15		yes	no	low hemat qpcr starting quant	box7b6
44	E7
45	E8
46	E9	6144_14_9	14	a	infected	4.92	1067			no	no	original tube from RNA extraction
47	F1	6211_481_26	481	a	infected	17.9	105477			yes	yes
48	F2	6104_259_12	259	c	uninfected	31.7	19			no	no
49	F3	6173_210_12	210	c	infected	33.3	838			no	no	low qPCR start quant of Hematodinium compared to other infected samples but high RNA concentration.
50	F4	6241_323_12	323	a	infected	22.2	15300			yes	no		box 7 g2
51	F5	6234_263_12	263	w	uninfected	26.6	0			no	no	not sure i trust qpcr hemato starting quant...
52	F6	6219_276_12	276	w	infected	4.2	652	45		no	no	can't find replacement tube for thisl
53	F7
54	F8
55	F9	6144_348_12	348	a	infected	5.76	1067			no	no	original tube from RNA extraction
56	G1	6273_127_9	127	w	infected	72	211000			yes	yes
57	G2	6189_216_12	216	c	uninfected	44.4	87			no	no
58	G3	6162_220_12	220	c	infected	12.9	879			no	no	low qPCR start quant of Hematodinium compared to other infected samples; not super high RNA concentration.
59	G4	6202_337_12	337	a	infected	23.7	1378			yes	no		box8a4
60	G5	6253_275_12	275	w	uninfected	29.8	0			no	no	not sure i trust qpcr hemato starting quant...
61	G6	6266_264_12	264	w	infected	40.5	199	15		no	no	low hemat qpcr starting quant, but high RNA concentration
62	G7
63	G8
64	G9	6144_477_26	477	a	infected	1.84	1067			no	no	original tube from RNA extraction
65	H1	6273_280_12	280	w	infected	17.9	211000			yes	yes
66	H2	6160_238_12	238	c	uninfected	38	29			no	no
67	H3	6279_219_12	219	c	infected	19.3	747			yes	no	low qPCR start quant of Hematodinium compared to other infected samples; not super high RNA concentration.
68	H4	6183_322_12	322	a	infected	2.69	1781	45	121.05	no	no	low RNA concentration, but high elution volume
69	H5	6265_282_12	282	w	uninfected	24.4	17			no	no
70	H6
71	H7
72	H8
73	H9
74	I1	6141_123_9	123	c	infected	13.1	6789	5	65.5	yes	no	aliquoted out for genewiz in 0.5 ul; not sent
75	I2	6153_209_12	209	c	uninfected	24.3	79			no	no
76	I3	6107_350_12	350	a	uninfected	20.4	61			yes	no		box 7 b3
77	I4	6115_307_12	307	a	infected	7.89	1460			yes	no		bx7F9
78	I5	6268_369_12	369	w	uninfected	31.5	0			yes	no	not sure i trust qpcr hemato starting quant...	box 7 b8
79	I6
80	I7
81	I8
82	I9
83
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86
87
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90
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