20150806_Jake_oly_mech_stress_cDNA_calcs

	A	B	C	D	E	F	G	H	I	J	K	L	M
1	Plate Position	Sample ID	[DNased RNA] (ng/ul)	uL to 250ng RNA	uL dT primers (0.5ug/ug RNA) of a 1:10 dilution	H2O to 74.75uL	5x rxn buffer (uL)	10mM dNTPs (uL)	MMLV (uL)	Master mix	Single Reaction (uL)	# Reactions	Total (uL)

2	A01	DNased 42215 HM1 1	28.72	8.70	1.25	64.80	20	5	0.25	5x buffer	20	27	540
3	B01	DNased 42215 HM1 2	22.24	11.24	1.25	62.26	20	5	0.25	dNTPs	5	27	135
4	C01	DNased 42215 HM1 3	25.14	9.94	1.25	63.56	20	5	0.25	MMLV	0.25	27	6.75
5	D01	DNased 42215 HM1 4	22.05	11.34	1.25	62.16	20	5	0.25
6	E01	DNased 42215 HM1 5	22.78	10.97	1.25	62.53	20	5	0.25
7	F01	DNased 42215 HM1 6	28.44	8.79	1.25	64.71	20	5	0.25
8	G01	DNased 42215 HM1 7	28.26	8.85	1.25	64.65	20	5	0.25
9	H01	DNased 42215 HM1 8	22.38	11.17	1.25	62.33	20	5	0.25
10	A02	DNased 42215 NM1 1	29.19	8.56	1.25	64.94	20	5	0.25
11	B02	DNased 42215 NM1 2	26.53	9.42	1.25	64.08	20	5	0.25
12	C02	DNased 42215 NM1 3	27.88	8.97	1.25	64.53	20	5	0.25
13	D02	DNased 42215 NM1 4	29.71	8.41	1.25	65.09	20	5	0.25
14	E02	DNased 42215 NM1 5	31.22	8.01	1.25	65.49	20	5	0.25
15	F02	DNased 42215 NM1 6	30.89	8.09	1.25	65.41	20	5	0.25
16	G02	DNased 42215 NM1 7	24.48	10.21	1.25	63.29	20	5	0.25
17	H02	DNased 42215 NM1 8	29.11	8.59	1.25	64.91	20	5	0.25
18	A03	DNased 42215 SM1 1	22.61	11.06	1.25	62.44	20	5	0.25
19	B03	DNased 42215 SM1 2	29.96	8.34	1.25	65.16	20	5	0.25
20	C03	DNased 42215 SM1 3	27.01	9.26	1.25	64.24	20	5	0.25
21	D03	DNased 42215 SM1 4	27.2	9.19	1.25	64.31	20	5	0.25
22	E03	DNased 42215 SM1 5	38.18	6.55	1.25	66.95	20	5	0.25
23	F03	DNased 42215 SM1 6	28.03	8.92	1.25	64.58	20	5	0.25
24	G03	DNased 42215 SM1 7	31.06	8.05	1.25	65.45	20	5	0.25
25	H03	DNased 42215 SM1 8	25.34	9.87	1.25	63.63	20	5	0.25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100