20151117_RAD_qPCR_data

	A	B	C	D	E	F	G	H	I	J	K	L	M	N	O	P
1	Well / Set	Dye	Content	Description	Efficiency	C(t)	pM	Avg C(t)	Max C(t)	Min C(t)	C(t) SD	Avg pM	Max pM	Min pM	pM SD	Wellset Type
2
3	NTC	SBGnew	Sample	NTC	21.99%	29.47	1.67E-06	29.45	29.68	29.22	0.19	1.72E-06	1.99E-06	1.46E-06	2.19E-07	Replicate
4	RAD 02	SBGnew	Sample	RAD02	97.28%	12.61	0.1715	12.68	13.25	12.19	0.44	0.1707	0.2284	0.1106	0.04811	Replicate
5	RAD 03	SBGnew	Sample	RAD03	92.35%	11.83	0.2914	11.84	12.07	11.69	0.17	0.2924	0.3203	0.2471	0.03234	Replicate
6	RAD04	SBGnew	Sample	RAD04	95.80%	12.21	0.2253	12.21	12.41	12.09	0.14	0.2254	0.245	0.1962	0.02106	Replicate
7	RAD06	SBGnew	Sample	RAD06	92.55%	13.23	0.1124	13.23	13.42	13.1	0.13	0.1124	0.1223	0.09868	0.01	Replicate
8	RAD07	SBGnew	Sample	RAD07	97.92%	9.21	1.752	9.23	9.58	9	0.26	1.756	2.026	1.357	0.2882	Replicate
9	RAD08	SBGnew	Sample	RAD08	102.48%	12.62	0.1701	12.64	12.82	12.36	0.2	0.1699	0.2036	0.1485	0.02413	Replicate
10	RAD14	SBGnew	Sample	RAD14	93.18%	10.31	0.826	10.33	10.61	10.08	0.22	0.824	0.9661	0.6733	0.1197	Replicate
11	RAD17	SBGnew	Sample	RAD17	95.81%	13.55	0.09018	13.56	13.87	13.31	0.24	0.09064	0.1063	0.07213	0.01408	Replicate
12	RAD23	SBGnew	Sample	RAD23	103.14%	12.52	0.182	12.57	12.97	12	0.41	0.1838	0.2607	0.1342	0.05511	Replicate
13	RAD30	SBGnew	Sample	RAD30	95.31%	14.7	0.04111	14.72	14.98	14.29	0.31	0.04141	0.05436	0.03379	0.009201	Replicate
14	Std1	SBGnew	Standard		101.14%	5.67	20	5.7	5.92	5.29	0.29	20	20	20	0	Replicate
15	Std2	SBGnew	Standard		96.81%	9.13	2	9.14	9.49	8.9	0.25	2	2	2	0	Replicate
16	Std3	SBGnew	Standard		95.25%	12.3	0.2	12.31	12.5	12.13	0.15	0.2	0.2	0.2	0	Replicate
17	Std4	SBGnew	Standard		92.98%	15.7	0.02	15.71	15.87	15.58	0.12	0.02	0.02	0.02	0	Replicate
18	Std5	SBGnew	Standard		74.72%	18.94	0.002	18.94	19.1	18.83	0.12	0.002	0.002	0.002	0	Replicate
19	Std6	SBGnew	Standard		68.43%	22.66	0.0002	22.68	22.92	22.38	0.23	0.0002	0.0002	0.0002	0	Replicate
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22												LIBRARY SUBMISSION CALCS
23	Library	Avg. Conc. (pM)	KAPA Standards Fragment Size (bp)	Library Fragment Size (bp)	Size-adjusted Conc. (pM)	qPCR Dilution Factor	Library Stock Conc. (pM)	Library Stock Conc. (nM)	Halved (nM)	Conc (ng/uL)	20ng (uL)	40ng (uL)	Amount	Conc
24	RAD 02	0.1715	452	166	0.4669759036	100000	46,697.59	46.70	23.35	4.71581687	4.24	8.48		4.427244982
25	RAD 03	0.2914	452	166	0.7934506024	100000	79,345.06	79.35	39.67	8.012764057	2.50	4.99
26	RAD04	0.2253	452	166	0.6134674699	100000	61,346.75	61.35	30.67	6.195180995	3.23	6.46
27	RAD06	0.1124	452	166	0.306053012	100000	30,605.30	30.61	15.30	3.090716129	6.47	12.94
28	RAD07	1.752	452	166	4.770506024	100000	477,050.60	477.05	238.53	48.17557525	0.42	0.83
29	RAD08	0.1701	452	166	0.4631638554	100000	46,316.39	46.32	23.16	4.677320405	4.28	8.55
30	RAD14	0.826	452	166	2.249108434	100000	224,910.84	224.91	112.46	22.7129139	0.88	1.76
31	RAD17	0.09018	452	166	0.2455503614	100000	24,555.04	24.56	12.28	2.479722247	8.07	16.13
32	RAD23	0.182	452	166	0.4955662651	100000	49,556.63	49.56	24.78	5.004540351	4.00	7.99
33	RAD30	0.04111	452	166	0.1119380723	100000	11,193.81	11.19	NA	1.130421175	17.69
34												85.83
35
36	Well / Set	Dye	Content	Description	Efficiency	C(t)	pM
37	A1	SBGnew	Sample	RAD02	91.19%	13.25	0.1106
38	A2	SBGnew	Sample	RAD02	97.13%	12.59	0.1732
39	A3	SBGnew	Sample	RAD02	98.84%	12.19	0.2284
40	A4	SBGnew	Sample	RAD03	94.87%	11.74	0.3099
41	A5	SBGnew	Sample	RAD03	84.76%	11.69	0.3203
42	A6	SBGnew	Sample	RAD03	91.29%	12.07	0.2471
43	A7	SBGnew	Sample	RAD04	96.92%	12.41	0.1962
44	A8	SBGnew	Sample	RAD04	95.54%	12.09	0.245
45	A9	SBGnew	Sample	RAD04	95.13%	12.15	0.2351
46	A10	SBGnew	Sample	RAD06	90.71%	13.18	0.1161
47	A11	SBGnew	Sample	RAD06	94.19%	13.1	0.1223
48	A12	SBGnew	Sample	RAD06	92.70%	13.42	0.09868
49	B1	SBGnew	Sample	RAD07	101.06%	9.58	1.357
50	B2	SBGnew	Sample	RAD07	112.08%	9	2.026
51	B3	SBGnew	Sample	RAD07	97.59%	9.1	1.886
52	B4	SBGnew	Sample	RAD08	103.76%	12.36	0.2036
53	B5	SBGnew	Sample	RAD08	100.24%	12.73	0.1575
54	B6	SBGnew	Sample	RAD08	103.12%	12.82	0.1485
55	B7	SBGnew	Sample	RAD14	89.67%	10.61	0.6733
56	B8	SBGnew	Sample	RAD14	95.79%	10.08	0.9661
57	B9	SBGnew	Sample	RAD14	93.06%	10.3	0.8327
58	B10	SBGnew	Sample	RAD17	92.16%	13.31	0.1063
59	B11	SBGnew	Sample	RAD17	94.79%	13.49	0.09355
60	B12	SBGnew	Sample	RAD17	102.71%	13.87	0.07213
61	C1	SBGnew	Sample	RAD23	109.52%	12.97	0.1342
62	C2	SBGnew	Sample	RAD23	107.87%	12.74	0.1566
63	C3	SBGnew	Sample	RAD23	112.07%	12	0.2607
64	C4	SBGnew	Sample	RAD30	92.01%	14.29	0.05436
65	C5	SBGnew	Sample	RAD30	113.49%	14.98	0.03379
66	C6	SBGnew	Sample	RAD30	85.14%	14.89	0.03609
67	C7	SBGnew	Standard	Std1	100.28%	5.92	20
68	C8	SBGnew	Standard	Std1	96.27%	5.29	20
69	C9	SBGnew	Standard	Std1	109.63%	5.87	20
70	C10	SBGnew	Standard	Std2	107.17%	8.9	2
71	C11	SBGnew	Standard	Std2	96.14%	9.05	2
72	C12	SBGnew	Standard	Std2	101.15%	9.49	2
73	D1	SBGnew	Standard	Std3	88.63%	12.5	0.2
74	D2	SBGnew	Standard	Std3	95.46%	12.29	0.2
75	D3	SBGnew	Standard	Std3	100.33%	12.13	0.2
76	D4	SBGnew	Standard	Std4	92.68%	15.68	0.02
77	D5	SBGnew	Standard	Std4	99.20%	15.58	0.02
78	D6	SBGnew	Standard	Std4	86.12%	15.87	0.02
79	D7	SBGnew	Standard	Std5	79.85%	19.1	0.002
80	D8	SBGnew	Standard	Std5	67.91%	18.83	0.002
81	D9	SBGnew	Standard	Std5	76.29%	18.88	0.002
82	D10	SBGnew	Standard	Std6	70.05%	22.38	0.0002
83	D11	SBGnew	Standard	Std6	68.09%	22.73	0.0002
84	D12	SBGnew	Standard	Std6	66.69%	22.92	0.0002
85	E1	SBGnew	Sample	NTC	19.95%	29.45	1.71E-06
86	E2	SBGnew	Sample	NTC	28.09%	29.68	1.46E-06
87	E3	SBGnew	Sample	NTC	18.58%	29.22	1.99E-06
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