FastQCFastQC Report
Mon 13 Apr 2015
2112_lane1_TTAGGC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_TTAGGC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16000000
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC170297810.643612500000001TruSeq Adapter, Index 3 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGT962970.60185625TruSeq Adapter, Index 3 (100% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC447730.27983125TruSeq Adapter, Index 3 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC347380.21711249999999999TruSeq Adapter, Index 3 (98% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC248320.1552TruSeq Adapter, Index 3 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC187500.1171875TruSeq Adapter, Index 3 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGAAG126400.084.7886663
GACCGGA123300.084.428991
ACCGGAA135200.078.991252
TCCGAAG53400.070.7247853
CGAAGAG62650.070.658315
GAGCACA3481350.062.3288239
GCGGAAG49350.062.1857873
ACGGAAG67100.061.098993
AGAGCAC3558600.060.978468
CATCGGA37700.060.1158941
CCGAAGA74250.059.5006074
AAGAGCA3653400.059.4546667
GAAGAGC3706550.058.629216
GAGCGGA50300.058.470251
AGCGGAA52100.058.266982
AATCATA1371450.056.4862025
GGAAGAG3781150.056.182955
GATTGGA86300.055.9364851
GAACGGA71350.055.7370261
TTGGAAG87550.055.183673
Produced by FastQC (version 0.11.2)