FastQCFastQC Report
Mon 13 Apr 2015
2112_lane1_TTAGGC_L001_R1_002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_TTAGGC_L001_R1_002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9752634
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC109977911.276738161198297TruSeq Adapter, Index 3 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC719880.7381390504349902TruSeq Adapter, Index 3 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGT643520.6598422538977675TruSeq Adapter, Index 3 (100% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC469990.48191083557529174TruSeq Adapter, Index 3 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC307570.31537121151065445TruSeq Adapter, Index 3 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC215790.22126330179108536TruSeq Adapter, Index 3 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA88900.086.835721
CCGGAAG94650.086.376563
ACCGGAA100200.081.308612
CATCGGA36550.070.706341
ACGGAAG49350.070.4627463
GATTGGA77950.069.354231
ATTGGAA78950.068.775572
TTGGAAG79700.068.42573
TGGAAGA87600.066.912754
GAGCACA2261950.063.2763569
GAACGGA53550.062.2765351
AGAGCAC2308200.062.0287328
AAGAGCA2363150.060.654727
GCGGAAG27350.060.4446073
GAAGAGC2400650.059.742866
AGCGGAA27300.059.6858832
GAGCGGA25500.059.6151471
AATCATA840850.057.86885
GGAAGAG2452200.057.4449235
CGGAAGA2429800.055.5371064
Produced by FastQC (version 0.11.2)