FastQCFastQC Report
Thu 8 Sep 2022
Geoduck-juvenile-ambient-exposure-RNA-EPI-123-6_S46_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeoduck-juvenile-ambient-exposure-RNA-EPI-123-6_S46_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1197
Sequences flagged as poor quality0
Sequence length45-151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC151.2531328320802004No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121.0025062656641603No Hit
TTCCGAAGACGGGAAGAAGCCGAGAGAGCAGTGAAGGATATCAACTTTAA70.5847953216374269No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG70.5847953216374269Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTACTTTGCGCAATAAGTGACTTGTAACCTGTTTTTTTATTGGGCTGC60.5012531328320802No Hit
TTTTTTGCATGGTTGAGTGGCTAATGAAGAACGACATAGTGATACTTTTT50.4177109440267335No Hit
GTTTACGAACTCGAAACTCTATTGTAACAGAGCTACAGTCTGTACAAAGA50.4177109440267335No Hit
TTTTAATCTACTTGTAAATGTTTTTATAAAATACATCTCATGACCAACAC50.4177109440267335No Hit
CTTTATCATAATTTGGCTTTATTAGTATTAGTTTTCATTATAAGGATAGT40.3341687552213868No Hit
TGACATTCGGTCTTTCCGGGGTATATAAACAGTTCCTTAATTGGATAGTT40.3341687552213868No Hit
CTTTAACTAATATACATATATTAACAATTCACCAACAGACACCATATACA30.2506265664160401No Hit
GCGGCTATTATTAAGAGTGATGCGTAAAATGAATACTCGACTGGAACCGG30.2506265664160401No Hit
TGTAAATGGCAAAATAAATGGGATTAAAAAGCATATAAATGGACATACGA30.2506265664160401No Hit
ATGCCTATTTCATCTTCATTCAGAACTAAATGTAGCCCTGATATTAGTAT20.1670843776106934No Hit
CCCAGGGCAGTATGATGGGACCAGGAATGCAGGGTGGTCCAAACAGCATG20.1670843776106934No Hit
GACACCCTACTAGCGTCTGGGAGTGCGCCAATCCAAATGTATATAGTGAA20.1670843776106934No Hit
CTGGTATGCTGTCAGATACCATTGTTTGGTATTATGTCCTCAGACTCTGC20.1670843776106934No Hit
AGAGGTAAAATAGCCAAGGCATCTGGTAATTATGCCACTGTCATCTCACA20.1670843776106934No Hit
TAGATATTGTCATATAGGATGGTATGTCAACAAAGATTCAAGTGTAGCTG20.1670843776106934No Hit
GTTGTCCCCAAGGATGTCAACGCCGCCATTGCTACCATCAAGACCAAGAG20.1670843776106934No Hit
GTCAAATACTCATTTAGGGATATATATATATATTCTGTGTATAAACAAAC20.1670843776106934No Hit
TAACTCATAAAACCCACCAATCTTATTTTCTTGTTGGTAGAGTGCCTGTA20.1670843776106934No Hit
CACATGCTTTCTGCTGTAAACCATATATTTTAAATGAATTTTAAATGAAT20.1670843776106934No Hit
GTTGATGATGAAGATGATATTTTTGGCCCTTATCATGTCAAGCGATTGTT20.1670843776106934No Hit
TAAAAATTGGTTTAAAAGTTAGTGAAGCATACAACTTAAGAACTTTAATA20.1670843776106934No Hit
CGTCGAGCCATACAACTCCATCCTGACCACCCATACCACCCTGGAGCACT20.1670843776106934No Hit
ATTTGGACTTGACCGAAGATTTGGAAGGAAACGTCAAATACGAAGAATGC20.1670843776106934No Hit
CGAGAACGTAATTTCGGTTCCCAGCAAGAAGGTAGACAACTGGGCGCTTC20.1670843776106934No Hit
CGTCAGTGAACGTACGGACAAATTTTCGACAAACTCTTTAAAAAACTTTT20.1670843776106934No Hit
AAAACTGTGTGTATGTCTGTTTACATCAACTGCCATGGCCGATTGGACAA20.1670843776106934No Hit
CGATGATGTTGCTGCTTTGGTTGTGGACAATGGATCCGGTATGTGTAAGG20.1670843776106934No Hit
GTCTTCCTCCTTTATTAGGGTCAGTTTTAAAATTGTATGGCGTTTTGGTG20.1670843776106934No Hit
CGTGTCTTCTATCGTGTACGATCTTCCTGCGCCCGTGAATATTTCTGCTA20.1670843776106934No Hit
CGTTCTTCTTGCTTTTTTTTAGATTATTTTTCGACAGTTTTTATCATTGT20.1670843776106934No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1670843776106934No Hit
TTTTTATCTGTTTTATTTCCTATATGGCTGATGGATCCAGAGAATTTTAA20.1670843776106934No Hit
GATGAAGAAGGTGATGATGAAGAAGACCATGATCATGACCACGATGAATT20.1670843776106934No Hit
GAATTGTTGAGGATTTCCAAGGACAAAAGAGCACTGAAGTTCTGTAAAAA20.1670843776106934No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT20.1670843776106934Illumina Single End PCR Primer 1 (100% over 50bp)
CTTCTTCCGTGTTTTTTTTCGTCATTCATTTTTTGTTAATTGTTGATCAT20.1670843776106934No Hit
CGAGTCCAGTGTGTCTGTGATTGTGATTTTGTATGAATCCTGGATATTGT20.1670843776106934No Hit
TTCACATGTAGCTGTAATGACGGATACGACGGGAACGGTGTCACTTGTAC20.1670843776106934No Hit
CCACTTTAAACAAGCCTCCAACTTCCTGTGGCCATTCAAATTGAACACGC20.1670843776106934No Hit
CAGTCAGCAGCCATCGTCGCAGCCGCAGCCGGATACTACAGCGCAGTCGG20.1670843776106934No Hit
CGGGTGTACGAGATTGTTTATTTCAGTGGGACATACTTTATGTGCCAGAT20.1670843776106934No Hit
ATTAATACCATATCAGAGTAACTGTGATAATGCTTGCGTTGTCAAATCCA20.1670843776106934No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)