Comments on: DNA Isolation – C.gigas larvae from 2011 NOAA OA Experiment http://onsnetwork.org/kubu4/2015/01/09/dna-isolation-c-gigas-larvae-from-2011-noaa-oa-experiment/ University of Washington - Fishery Sciences - Roberts Lab Tue, 09 Apr 2019 18:37:58 +0000 hourly 1 http://wordpress.org/?v=4.0 By: DNA Quantification – C.gigas Larvae 1000ppm | Sam's Notebook http://onsnetwork.org/kubu4/2015/01/09/dna-isolation-c-gigas-larvae-from-2011-noaa-oa-experiment/#comment-268 Fri, 27 Feb 2015 04:19:33 +0000 http://onsnetwork.org/kubu4/?p=9#comment-268 […] DNA pool, I decided to try to recover any residual DNA left in the 1B2 sample. Sample 1B2 (sheared on 20150109) was dry, so I added 20μL of Buffer EB (Qiagen) to the tube. I vortexed both the 1B1 and 1B2 […]

]]> By: Library Prep – Quantification of C.gigas larvae OA 1000ppm library | Sam's Notebook http://onsnetwork.org/kubu4/2015/01/09/dna-isolation-c-gigas-larvae-from-2011-noaa-oa-experiment/#comment-210 Tue, 10 Feb 2015 00:57:10 +0000 http://onsnetwork.org/kubu4/?p=9#comment-210 […] something is totally shot with this sample?  Will quantify the sheared DNA and decide what to […]

]]> By: Bisulfite NGS Library Prep – Bisulfite Conversion & Illumina Library Construction of C.gigas larvae DNA | Sam's Notebook http://onsnetwork.org/kubu4/2015/01/09/dna-isolation-c-gigas-larvae-from-2011-noaa-oa-experiment/#comment-204 Sat, 07 Feb 2015 00:48:54 +0000 http://onsnetwork.org/kubu4/?p=9#comment-204 […] previous attempt at constructing a library for the 1000ppm larvae samples failed. I had previously sheared, quantified, and concentrated the DNA from this sample. As I had done previously, I combined 50ng from each of […]

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