Comments on: RNA Isolation – Geoduck Gonad in Paraffin Histology Blocks http://onsnetwork.org/kubu4/2015/04/27/rna-isolation-geoduck-gonad-in-paraffin-histology-blocks-2/ University of Washington - Fishery Sciences - Roberts Lab Tue, 09 Apr 2019 18:37:58 +0000 hourly 1 http://wordpress.org/?v=4.0 By: Bioanalyzer – Geoduck Gonad RNA Quality Assessment | Sam's Notebook http://onsnetwork.org/kubu4/2015/04/27/rna-isolation-geoduck-gonad-in-paraffin-histology-blocks-2/#comment-1128 Tue, 30 Jun 2015 18:24:31 +0000 http://onsnetwork.org/kubu4/?p=1286#comment-1128 […] that was previously isolated on 20150508, 20150505, 20150427, and 20150424 (those notebook entries have been updated to report this consolidation and have a […]

]]> By: RNA Isolation – Geoduck Gonad in Paraffin Histology Blocks | Sam's Notebook http://onsnetwork.org/kubu4/2015/04/27/rna-isolation-geoduck-gonad-in-paraffin-histology-blocks-2/#comment-599 Tue, 05 May 2015 22:35:19 +0000 http://onsnetwork.org/kubu4/?p=1286#comment-599 […] Last week’s RNA isolation (a second attempt at obtaining RNA from the samples) performed poorly. I will re-isolate RNA from the following samples: […]

]]> By: Goals – May 2015 | Sam's Notebook http://onsnetwork.org/kubu4/2015/04/27/rna-isolation-geoduck-gonad-in-paraffin-histology-blocks-2/#comment-588 Fri, 01 May 2015 20:44:50 +0000 http://onsnetwork.org/kubu4/?p=1286#comment-588 […] primary goal for this project is to successfully isolate RNA from the remaining, troublesome paraffin blocks that have yet to yield any usable RNA. The next approach to obtain usable quantities of RNA is to directly gouge tissue from the blocks […]

]]> By: kubu4 http://onsnetwork.org/kubu4/2015/04/27/rna-isolation-geoduck-gonad-in-paraffin-histology-blocks-2/#comment-587 Fri, 01 May 2015 19:52:45 +0000 http://onsnetwork.org/kubu4/?p=1286#comment-587

I know we discussed this in person, but I’ll post here for posterity.

As far as I can tell from her notebook, there didn’t seem to be any issues. However, most of the samples that I have been unable to get RNA from are from the very first set that she prepped with the PAXgene Tissue Stabilizer. Could just be coincidence.

Anyway, as discussed, I’ll try taking some gouges of tissue directly from those blocks and running them through the RNA isolation procedure again.

]]> By: Steven http://onsnetwork.org/kubu4/2015/04/27/rna-isolation-geoduck-gonad-in-paraffin-histology-blocks-2/#comment-574 Tue, 28 Apr 2015 01:41:33 +0000 http://onsnetwork.org/kubu4/?p=1286#comment-574 Hindsight- she did have some issues with proper preservation on some occasions- using wrong sequence of chemicals and/or not getting into building?

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