Comments on: RNA Isolation – Jake’s O. lurida Ctenidia 1hr Heat Stress from 20150422 http://onsnetwork.org/kubu4/2015/05/06/rna-isolation-jakes-o-lurida-ctenidia-1hr-heat-stress-from-20150422/ University of Washington - Fishery Sciences - Roberts Lab Tue, 09 Apr 2019 18:37:58 +0000 hourly 1 http://wordpress.org/?v=4.0 By: DNase Treatment – Jake’s O.lurida Ctenidia RNA (1hr Heat Shock) from 20150506 | Sam's Notebook http://onsnetwork.org/kubu4/2015/05/06/rna-isolation-jakes-o-lurida-ctenidia-1hr-heat-stress-from-20150422/#comment-701 Fri, 15 May 2015 00:47:09 +0000 http://onsnetwork.org/kubu4/?p=1313#comment-701 […] the O.lurida RNA I isolated on 20150506 showed residual gDNA via qPCR, I treated 1.5μg of RNA from each sample using the Turbo DNA-free […]

]]> By: qPCR – Jake O.lurida ctenidia RNA (Heat Shock Samples) from 20150506 | Sam's Notebook http://onsnetwork.org/kubu4/2015/05/06/rna-isolation-jakes-o-lurida-ctenidia-1hr-heat-stress-from-20150422/#comment-682 Wed, 13 May 2015 00:43:02 +0000 http://onsnetwork.org/kubu4/?p=1313#comment-682 […] qPCRs on the O.lurida total RNA I isolated on 20150506 to assess presence of gDNA carryover with Oly Actin primers (SR IDs: 1505, […]

]]> By: RNA Isolation – Jake’s O. lurida Ctenidia Control from 20150422 | Sam's Notebook http://onsnetwork.org/kubu4/2015/05/06/rna-isolation-jakes-o-lurida-ctenidia-1hr-heat-stress-from-20150422/#comment-628 Thu, 07 May 2015 20:37:51 +0000 http://onsnetwork.org/kubu4/?p=1313#comment-628 […] pretty solid 260/280 ratios (>1.85). Interestingly, the 260/230 ratios aren’t so great (compared to yesterday’s isolations). I suspect that the reason for this is that there appeared to be more starting tissue in these […]

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