Comments on: Restriction Digest – Oly gDNA for RAD-seq w/AlfI http://onsnetwork.org/kubu4/2015/10/28/restriction-digest-oly-gdna-for-rad-seq-walfi-3/ University of Washington - Fishery Sciences - Roberts Lab Tue, 09 Apr 2019 18:37:58 +0000 hourly 1 http://wordpress.org/?v=4.0 By: Adaptor Ligation – Oly AlfI-Digested gDNA for RAD-seq | Sam's Notebook http://onsnetwork.org/kubu4/2015/10/28/restriction-digest-oly-gdna-for-rad-seq-walfi-3/#comment-2363 Fri, 30 Oct 2015 00:04:41 +0000 http://onsnetwork.org/kubu4/?p=1741#comment-2363 […] Digested DNA from yesterday was heat inactivated for 10mins @ 65C and was not run out on a gel due to the fact that the input gDNA was degraded and a shift in the high molecular weight band (indicating the digestion was successful) would not exist because a high molecular weight band is absent in these samples. […]

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