Comments on: FASTQC – Oly BGI GBS Raw Illumina Data http://onsnetwork.org/kubu4/2017/03/02/fastqc-oly-bgi-gbs-raw-illumina-data/ University of Washington - Fishery Sciences - Roberts Lab Tue, 09 Apr 2019 18:37:58 +0000 hourly 1 http://wordpress.org/?v=4.0 By: FASTQC – Oly BGI GBS Raw Illumina Data Demultiplexed | Sam's Notebook http://onsnetwork.org/kubu4/2017/03/02/fastqc-oly-bgi-gbs-raw-illumina-data/#comment-4840 Tue, 07 Mar 2017 17:47:07 +0000 http://onsnetwork.org/kubu4/?p=2497#comment-4840 […] Last week, I ran the two raw FASTQ files through FastQC. As expected, FastQC detected “errors”. These errors are due to the presence of adapter sequences, barcodes, and the use of a restriction enzyme (ApeKI) in library preparation. In summary, it’s not surprising that FastQC was not please with the data because it’s expecting a “standard” library prep that’s already been trimmed and demultiplexed. […]

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