Comments on: Genome Assembly – minimap/miniasm/racon Overview http://onsnetwork.org/kubu4/2017/10/04/genome-assembly-minimapminiasmracon-overview/ University of Washington - Fishery Sciences - Roberts Lab Tue, 09 Apr 2019 18:37:58 +0000 hourly 1 http://wordpress.org/?v=4.0 By: tom http://onsnetwork.org/kubu4/2017/10/04/genome-assembly-minimapminiasmracon-overview/#comment-7137 Tue, 09 Apr 2019 18:37:58 +0000 http://onsnetwork.org/kubu4/?p=2744#comment-7137 In case somebody else finds this (like i did today) and despairs because it doesn’t work (like i did today):

I’m pretty sure there is an error in step 4. You don’t “map the fastq reads back to the contigs (the PAF file) created in the first step”. You instead map the fastq reads against the converted assembly from step 3.

The example given would suggest mapping the alignment map from step 1 against the assembly created in step 2. Which is not what the text says, but doesn’t look correct either.

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