Sam's Notebook » 2L HKOD09 http://onsnetwork.org/kubu4 University of Washington - Fishery Sciences - Roberts Lab Thu, 08 Nov 2018 21:47:12 +0000 en-US hourly 1 http://wordpress.org/?v=4.0 RNA Precipitation – Herring Liver RNA for SOLiD Libraries http://onsnetwork.org/kubu4/2009/12/01/rna-precipitation-herring-liver-rna-for-solid-libraries/ http://onsnetwork.org/kubu4/2009/12/01/rna-precipitation-herring-liver-rna-for-solid-libraries/#comments Wed, 02 Dec 2009 01:15:02 +0000 http://onsnetwork.org/kubu4/?p=782

50uL of RNA from each of the following were precipitated O/N @ -20C:

2L HKOD09 – 2.157 ug/uL x 50uL = 107.85ug

4L HTOG09 – 2.089 ug/uL x 50uL = 104.45ug

3L HSITK09 – 1.089 ug/uL x 50uL = 54.45ug

6L HPWS09 – 1.703 ug/uL x 50uL = 85.15ug

0.1 volumes (5uL) of 3M NaOAc ph = 5.2 was added to each sample. Then 2 volumes (110uL) of 100% EtOH was added. Samples were vortexed and incubated O/N @ -20C.

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