Sam's Notebook » 5-mC monoclonal antibody cl. b http://onsnetwork.org/kubu4 University of Washington - Fishery Sciences - Roberts Lab Thu, 08 Nov 2018 21:47:12 +0000 en-US hourly 1 http://wordpress.org/?v=4.0 MeDIP – SB/WB Fragmented gDNA (from 20100625) http://onsnetwork.org/kubu4/2010/06/30/medip-sbwb-fragmented-gdna-from-20100625/ http://onsnetwork.org/kubu4/2010/06/30/medip-sbwb-fragmented-gdna-from-20100625/#comments Thu, 01 Jul 2010 04:54:55 +0000 http://onsnetwork.org/kubu4/?p=625

After confirming proper fragmentation (~460bp average fragment size) via Bioanalyzer earlier today, began the MeDIP process. Brought fragmented DNA samples up to 350uL with TE. Heated samples @ 95C for 10mins, then incubated on ice 5mins. Added 100uL of 5x MeDIP Buffer (50mM Na2HPO4, 700mM NaCl, 0.25% Triton-X 100), 45uL of TE and 5uL (5ug) of anti-methyl cytidine antibody (Diagenode; 5-mC monoclonal antibody cl. b). Incubated O/N, 4C rotating.

]]> http://onsnetwork.org/kubu4/2010/06/30/medip-sbwb-fragmented-gdna-from-20100625/feed/ 0