Sam's Notebook » ATCC http://onsnetwork.org/kubu4 University of Washington - Fishery Sciences - Roberts Lab Thu, 08 Nov 2018 21:47:12 +0000 en-US hourly 1 http://wordpress.org/?v=4.0 Bacteria – C. pugettii culture http://onsnetwork.org/kubu4/2009/04/19/bacteria-c-pugettii-culture/ http://onsnetwork.org/kubu4/2009/04/19/bacteria-c-pugettii-culture/#comments Sun, 19 Apr 2009 18:59:11 +0000 http://onsnetwork.org/kubu4/?p=1113

Rehydrated ATCC isolate according to directions. Added 5mL of 1x Marine Broth to a glass culture tube. Used 1mL of this to rehydrate ATCC sample and then added back into the culture tube. Added a few crystals of biphenyl, which had been exposed to UV for ~5mins prior. Incubated at 20C, no shaking. This was done at ~11:30AM.

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