Sam's Notebook » BC05Ca18c/H5 http://onsnetwork.org/kubu4 University of Washington - Fishery Sciences - Roberts Lab Thu, 08 Nov 2018 21:47:12 +0000 en-US hourly 1 http://wordpress.org/?v=4.0 gDNA Isolation – New Dungan isolates http://onsnetwork.org/kubu4/2009/04/02/gdna-isolation-new-dungan-isolates/ http://onsnetwork.org/kubu4/2009/04/02/gdna-isolation-new-dungan-isolates/#comments Thu, 02 Apr 2009 23:51:26 +0000 http://onsnetwork.org/kubu4/?p=1157

gDNA was isolated from the following using the Qiagen DNEasy Kit:

xCvC-19t (3/26/2009)

xCvE-13t (3/16/2009)

xCvC-17t (3/18/2009)

UNTc-1.5t (3/18/2009)

VATm-1.2t (3/16/2009)

xCvC-11t (3/18/2009)

BC05Ca18c/H5 (3/27/2009)

xCvC-12t (3/16/2009)

500uL was of each sample was transferred to a 1.5mL snap cap tube. The samples were pelleted by spinning 5 mins @ 16,000g and washed 2x w/ 1x PBS pH=7.6. Samples were then processed according to Qiagen protocol. Initial digestion with Proteinase K was 2hrs.

]]>
http://onsnetwork.org/kubu4/2009/04/02/gdna-isolation-new-dungan-isolates/feed/ 0