Sam's Notebook » Bioruptor 300 http://onsnetwork.org/kubu4 University of Washington - Fishery Sciences - Roberts Lab Thu, 08 Nov 2018 21:47:12 +0000 en-US hourly 1 http://wordpress.org/?v=4.0 DNA Sonication – Oly gDNA for MBD http://onsnetwork.org/kubu4/2015/11/19/dna-sonication-oly-gdna-for-mbd/ http://onsnetwork.org/kubu4/2015/11/19/dna-sonication-oly-gdna-for-mbd/#comments Fri, 20 Nov 2015 04:47:07 +0000 http://onsnetwork.org/kubu4/?p=1794

In preparation for MBD enrichment, fragmented Olympia oyster gDNA with a target size of ~350bp.

Genomic DNA samples were isolated and provided to us by Katherine Silliman at UIC. Selected samples will compare Hood Canal (HC) and Oyster Bay (SS, South Sound) populations.

Used the Seeb Lab’s Bioruptor 300 (Diagenode) sonicator.

After sonication, samples were run on a the Seeb Lab’s 2100 Bioanalyzer (Agilent) on DNA 12000 chips.

Results:

HOOD CANAL SAMPLES

 

OYSTER BAY SAMPLES

More detailed analysis (including average fragment size for each samples) will be coming soon…

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