Sam's Notebook » bleeding http://onsnetwork.org/kubu4 University of Washington - Fishery Sciences - Roberts Lab Thu, 08 Nov 2018 21:47:12 +0000 en-US hourly 1 http://wordpress.org/?v=4.0 Bleeding/Tissue Collection – Hard Clams http://onsnetwork.org/kubu4/2009/01/20/bleedingtissue-collection-hard-clams/ http://onsnetwork.org/kubu4/2009/01/20/bleedingtissue-collection-hard-clams/#comments Wed, 21 Jan 2009 03:16:16 +0000 http://onsnetwork.org/kubu4/?p=1207

Hemos were collected from the remaining 12 clams as before and stored @ -80C. NOTE: 4 hemocyte samples were extremely cloudy. Possibly not hemos? Maybe gonad/d.g.? Gill tissue was collected from 5 clams and stored @ -80C.

]]> http://onsnetwork.org/kubu4/2009/01/20/bleedingtissue-collection-hard-clams/feed/ 0 Bleeding – Hard Clams http://onsnetwork.org/kubu4/2009/01/14/bleeding-hard-clams/ http://onsnetwork.org/kubu4/2009/01/14/bleeding-hard-clams/#comments Wed, 14 Jan 2009 22:59:30 +0000 http://onsnetwork.org/kubu4/?p=1213

Bled 7 clams from 20090108 and 20090109. Bled clams using a 23g 1.5 needle on a 3mL syringe. Fluid was gathered and ranged from ~0.4-1.0mL. Hemolymph was transferred to individual 1.5mL snap cap tubes and spun @ 100g for 30mins @ 4C. Most of the supe was removed, but left ~100uL in each tube to avoid disturbing any pellet. Samples were stored @ -80C in the red box with previous hard clam hemo samples.

NOTE: One sample was EXTREMELY cloudy. Likely not hemos.

]]> http://onsnetwork.org/kubu4/2009/01/14/bleeding-hard-clams/feed/ 0 Bleeding – Hard Clams http://onsnetwork.org/kubu4/2009/01/12/bleeding-hard-clams-2/ http://onsnetwork.org/kubu4/2009/01/12/bleeding-hard-clams-2/#comments Mon, 12 Jan 2009 23:03:31 +0000 http://onsnetwork.org/kubu4/?p=1219

Bled 8 clams from 20090108 and 20090109, #4, 6, 8, 15, 16, 17, 21, 26. Bled clams using a 23g 1.5 needle on a 3mL syringe. Fluid was gathered and ranged from ~0.4-1.0mL. Hemolymph was transferred to individual 1.5mL snap cap tubes and spun @ 100g for 30mins @ 4C. Most of the supe was removed, but left ~100uL in each tube to avoid disturbing any pellet. Samples were stored @ -80C in the red box with previous hard clam hemo samples.

Pellets were apparent in all 8 samples, whereas they had not been noticeable before in last week’s bleeds.

Also, 3 clams were found with cracked shells, but alive, including the one pictured below that is split open entirely.

]]> http://onsnetwork.org/kubu4/2009/01/12/bleeding-hard-clams-2/feed/ 0 Bleeding – Hard Clams http://onsnetwork.org/kubu4/2009/01/09/bleeding-hard-clams-3/ http://onsnetwork.org/kubu4/2009/01/09/bleeding-hard-clams-3/#comments Fri, 09 Jan 2009 23:04:44 +0000 http://onsnetwork.org/kubu4/?p=1221

Bled 24 hard clams using a 23g 1.5 needle on a 3mL syringe. Fluid was gathered and ranged from ~0.4-1.0mL. Hemolymph was transferred to individual 1.5mL snap cap tubes and spun @ 100g for 30mins @ 4C. Most of the supe was removed, but left ~100uL in each tube to avoid disturbing any pellet. Samples were stored @ -80C in the red box wiht previous hard clam hemo samples. Clams were numbered and transferred to a holding tank. Gill and mantle tissue was collected from 10 of the clams. The collected tissue and the rest of the carcasses were stored @ -80C.

]]> http://onsnetwork.org/kubu4/2009/01/09/bleeding-hard-clams-3/feed/ 0 Bleeding – Hard Clams http://onsnetwork.org/kubu4/2009/01/08/bleeding-hard-clams-4/ http://onsnetwork.org/kubu4/2009/01/08/bleeding-hard-clams-4/#comments Thu, 08 Jan 2009 23:05:49 +0000 http://onsnetwork.org/kubu4/?p=1223

Bled 6 hard clams using a 23g 1.5 needle on a 3mL syringe. Fluid was gathered and ranged from ~0.4-1.0mL. Hemolymph was transferred to individual 1.5mL snap cap tubes and spun @ 100g for 30mins @ 4C. Most of the supe was removed, but left ~100uL in each tube to avoid disturbing any pellet. Samples were stored @ -80C in the red box with previous hard clam hemo samples. Clams were numbered and transferred to a holding tank.

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