Sam's Notebook » caligus tape http://onsnetwork.org/kubu4 University of Washington - Fishery Sciences - Roberts Lab Thu, 08 Nov 2018 21:47:12 +0000 en-US hourly 1 http://wordpress.org/?v=4.0 DNA Quantification – Sea Lice DNA from 20180523 http://onsnetwork.org/kubu4/2018/09/12/dna-quantification-sea-lice-dna-from-20180523/ http://onsnetwork.org/kubu4/2018/09/12/dna-quantification-sea-lice-dna-from-20180523/#comments Wed, 12 Sep 2018 20:52:08 +0000 http://onsnetwork.org/kubu4/?p=3545

We previously received sea lice (Caligus tape) DNA from Cris Gallardo-Escarate at Universidad de Concepción.

Steven asked that I quantify and assess the DNA quality.

Ran the samples on the Roberts Lab Qubit 3.0 using the dsDNA BR assay (Invitrogen) and 1uL of template DNA.

Ran the samples on the Roberts Lab NanoDrop1000 to get 260/280 values for quality assessment using 2uL of template DNA. NanoDrop1000 was blanked with water, but I don’t know what solvent the DNA is currently resuspended in.

RESULTS

Qubit data (Google Sheet):

SAMPLE CONCENTRATION (ng/uL)
FEMALE 1 23.8
FEMALE 2 9.6

NANODROP DATA

TABLE

ABSORBANCE PLOTS

DNA looks super clean. Not sure what this DNA is intended for so can’t speculate much on what the implications might be for the concentrations on downstream usage.

]]> http://onsnetwork.org/kubu4/2018/09/12/dna-quantification-sea-lice-dna-from-20180523/feed/ 0 DNA Received – Sea lice DNA from Cris Gallardo-Escarate at Universidad de Concepción http://onsnetwork.org/kubu4/2018/05/23/dna-received-sea-lice-dna-from-cris-gallardo-escarate-at-universidad-de-concepcion/ http://onsnetwork.org/kubu4/2018/05/23/dna-received-sea-lice-dna-from-cris-gallardo-escarate-at-universidad-de-concepcion/#comments Wed, 23 May 2018 18:15:55 +0000 http://onsnetwork.org/kubu4/?p=3379

Received Caligus tape DNA – two samples:

  • Female 1 .

  • Female 2 .

Stored in slots H4 and H5 in “Sam’s gDNA Box #2″ in the FTR 213 -20oC freezer.

Google Sheet: Sam’s gDNA Box #2

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