Sam's Notebook » Cg_HIF1 http://onsnetwork.org/kubu4 University of Washington - Fishery Sciences - Roberts Lab Thu, 08 Nov 2018 21:47:12 +0000 en-US hourly 1 http://wordpress.org/?v=4.0 qPCRs – Tim’s Adult Gigas gill cDNA (from 20091009) http://onsnetwork.org/kubu4/2010/01/05/qpcrs-tims-adult-gigas-gill-cdna-from-20091009-2/ http://onsnetwork.org/kubu4/2010/01/05/qpcrs-tims-adult-gigas-gill-cdna-from-20091009-2/#comments Tue, 05 Jan 2010 20:16:58 +0000 http://onsnetwork.org/kubu4/?p=722

Duplicates of earlier qPCRs.

Primers: Cg_HIF1 and IL17 Iso D.

The previous version of IL17 primers used (IL17 internal) were NOT the ones used for the paper. The IL17 Iso D are the correct primers and are the same ones that Tim previously used on the juvenille gill samples.

qPCR set up and plate layout can be found here.

Results:

Duplicates of earlier qPCRs.

Primers: EF1 and IL17 Iso D.

The previous version of IL17 primers used (IL17 internal) were NOT the ones used for the paper. The IL17 Iso D are the correct primers and are the same ones that Tim previously used on the juvenille gill samples. qPCR set up and plate layout can be found here.

Results:

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