Sam's Notebook » Clupea pallasii http://onsnetwork.org/kubu4 University of Washington - Fishery Sciences - Roberts Lab Thu, 08 Nov 2018 21:47:12 +0000 en-US hourly 1 http://wordpress.org/?v=4.0 Reverse Transcription – Herring RNA from 20091026 http://onsnetwork.org/kubu4/2013/02/13/reverse-transcription-herring-rna-from-20091026/ http://onsnetwork.org/kubu4/2013/02/13/reverse-transcription-herring-rna-from-20091026/#comments Wed, 13 Feb 2013 23:24:07 +0000 http://onsnetwork.org/kubu4/?p=202

Performed an RT reaction on pooled herring gonad and liver mRNA from 20091026 for James Raymond at the UNLV. A single RT reaction was performed using 12.75uL (208ng) of the pooled gonad mRNA and 5uL (132.5ng) of the pooled liver RNA, according to our default MMLV (Promega) protocol. After reaction was completed, sample was stored @ -20C and then shipped to James Raymond on 20130214.

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