Sam's Notebook » D11-C http://onsnetwork.org/kubu4 University of Washington - Fishery Sciences - Roberts Lab Thu, 08 Nov 2018 21:47:12 +0000 en-US hourly 1 http://wordpress.org/?v=4.0 RNA Quantification – Ronit’s C.gigas Ploidy/Dessication RNA http://onsnetwork.org/kubu4/2018/10/16/rna-quantification-ronits-c-gigas-ploidydessication-rna/ http://onsnetwork.org/kubu4/2018/10/16/rna-quantification-ronits-c-gigas-ploidydessication-rna/#comments Tue, 16 Oct 2018 15:14:51 +0000 http://onsnetwork.org/kubu4/?p=3642

Last Friday, Ronit quantified 1:10 dilutions of the RNA I isolated on 20181003 and the RNA he finished isolating on 20181011, but two of the samples (D11-C, T10-C) were still too concentrated.

I made 1:20 dilutions (1uL RNA in 19uL 0.1% DEPC-treated H2O) and quantified them using the Roberts Lab Qubit 3.0, with the RNA HS assay. Used 1uL of the diluted RNA.

RESULTS

Qubit data (Google Sheet):

Everything looks good. Added final concentration values (Qubit data x 20, to account for dilution factor) to Ronit’s master sheet (Google Sheet):

Will proceed with DNasing.

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