Sam's Notebook » DEF1 http://onsnetwork.org/kubu4 University of Washington - Fishery Sciences - Roberts Lab Thu, 08 Nov 2018 21:47:12 +0000 en-US hourly 1 http://wordpress.org/?v=4.0 qPCRs – BB & DH cDNA (from 20091223) http://onsnetwork.org/kubu4/2009/12/30/qpcrs-bb-dh-cdna-from-20091223/ http://onsnetwork.org/kubu4/2009/12/30/qpcrs-bb-dh-cdna-from-20091223/#comments Wed, 30 Dec 2009 23:28:28 +0000 http://onsnetwork.org/kubu4/?p=726

qPCR was set up on these cDNAs using the following primers:

AJ582629.p.cg.6 (“DEF1″, “Defensin 1″) – This was upregulated in BB SOLiD data.

CB617519.p.cg.6 (“RETST”, “All-trans retinol”) – This was upregulated in BB SOLiD data.

qPCR set up and plate layout can be found here.

Results:

qPCR Data File (Opticon): 20091230_173747.tad

Data workup: PROPS BB_DH Gene Expression Miner Workup

 

 

qPCR was set up on these cDNAs using the following primers:

CU684779.p.cg.6 (“SEMSA”, “Semaphorin-SA”) – This was upregulated in BB SOLiD data.

FP004879.p.cg.6 (“TIMP3″, “Metalloproteinase inhibitor 3″) – This was upregulated in BB SOLiD data.

qPCR set up and plate layout can be found here.

Results:

qPCR Data File (Opticon): 20091230_143643.tad

Data workup: PROPS BB_DH Gene Expression Miner Workup

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