Sam's Notebook » Dermo http://onsnetwork.org/kubu4 University of Washington - Fishery Sciences - Roberts Lab Thu, 08 Nov 2018 21:47:12 +0000 en-US hourly 1 http://wordpress.org/?v=4.0 gDNA Isolation – Gigas Dermo Samples http://onsnetwork.org/kubu4/2009/05/15/gdna-isolation-gigas-dermo-samples/ http://onsnetwork.org/kubu4/2009/05/15/gdna-isolation-gigas-dermo-samples/#comments Fri, 15 May 2009 16:51:44 +0000 http://onsnetwork.org/kubu4/?p=1067

Transferred tissue from previously Chelexed samples:

WP3, WP7, WP13, WP14, WP15, CY1 – These all tested negative for virginica gDNA (18s qPCR by Rony on DATE)

CY42, CY36 – These tested positive for virginica gDNA (18s qPCR by Rony on DATE).

Used Qiagen DNeasy Kit. Digested samples for 3 hrs. at 55C in Proteinase K according to protocol.

Results: Horrible. Virtually no DNA in any of these samples. Additionally, the quality of the DNA is atrocious. Not sure what to do now.

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