Sam's Notebook » FIU http://onsnetwork.org/kubu4 University of Washington - Fishery Sciences - Roberts Lab Thu, 08 Nov 2018 21:47:12 +0000 en-US hourly 1 http://wordpress.org/?v=4.0 DNA Concentration – Acropora cervicornis (Staghorn coral) DNA from Javier Casariego (FIU) http://onsnetwork.org/kubu4/2017/05/10/dna-concentration-acropora-cervicornis-staghorn-coral-dna-from-javier-casariego-fiu/ http://onsnetwork.org/kubu4/2017/05/10/dna-concentration-acropora-cervicornis-staghorn-coral-dna-from-javier-casariego-fiu/#comments Wed, 10 May 2017 22:22:17 +0000 http://onsnetwork.org/kubu4/?p=2573

Three samples (of the 62 total) that were quantified earlier today, had concentrations too low for use in the methylation assay:

  • 2h Block 1
  • 2h Block 8
  • D35 Block 8

These samples were dried to completion in a SpeedVac.

They will be allowed to rehydrate O/N in 10uL of Buffer EB (Qiagen) and will be re-quantified tomorrow morning.

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Received 62 coral (Acropora cervicornis) DNA samples from Javier Casariego at FIU.

Spreadsheet of samples and NanoDrop concentrations provided by Javier (converted to Google Sheet): A.cervicornis_DNA_Extractions(May_2017).xlsx

Samples were temporarily stored at 4c (in FTR 213) until I can perform global methylation assessment on them tomorrow.

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