Sam's Notebook » GSTO1 http://onsnetwork.org/kubu4 University of Washington - Fishery Sciences - Roberts Lab Thu, 08 Nov 2018 21:47:12 +0000 en-US hourly 1 http://wordpress.org/?v=4.0 qPCR – BB & DH cDNA (from 20091223) http://onsnetwork.org/kubu4/2009/12/31/qpcr-bb-dh-cdna-from-20091223/ http://onsnetwork.org/kubu4/2009/12/31/qpcr-bb-dh-cdna-from-20091223/#comments Thu, 31 Dec 2009 23:24:05 +0000 http://onsnetwork.org/kubu4/?p=724

qPCR was set up on these cDNAs using the following primers:

FP008495.p.cg.6 (“GSTO1″, “Glutathione S-transferase omega-1″) – This was upregulated in BB SOLiD data.

These were run in duplicate to take up a full PCR plate. qPCR set up and plate layout can be found here.

Results:

qPCR Data File (Opticon): 20091231_152520.tad

Data workup: PROPS BB_DH Gene Expression Miner Workup

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