Sam's Notebook » H.crach_16s http://onsnetwork.org/kubu4 University of Washington - Fishery Sciences - Roberts Lab Thu, 08 Nov 2018 21:47:12 +0000 en-US hourly 1 http://wordpress.org/?v=4.0 qPCR – Abalone gDNA http://onsnetwork.org/kubu4/2009/07/15/qpcr-abalone-gdna/ http://onsnetwork.org/kubu4/2009/07/15/qpcr-abalone-gdna/#comments Thu, 16 Jul 2009 02:44:10 +0000 http://onsnetwork.org/kubu4/?p=946

Used up the remainder of the one positive control gDNA that worked with all the primers in yesterday’s reaction (H.crach_h-1fg_intron, H.iris_actin_intron, H.crach_16s), so need to find a new set of gDNA to use for future positive controls. qPCR plate layout/set up is here. Anneal temp 50C. Used the following gDNA with :

06:50-9 – This was the good gDNA used as previous controls. Added 10uL of H2O to the tube in hopes of getting more useable DNA.

06:4-7 – No date/info available on tube.

07:12-15 – No date/info available on tube.

Results: Got decent signals with the H.crach_h-1fg primers for two of the three gDNAs. Will use the 07:12-15 gDNA as a positive control for tomorrow’s qPCR.

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