Sam's Notebook » H.crach_16s_SYB_F/R http://onsnetwork.org/kubu4 University of Washington - Fishery Sciences - Roberts Lab Thu, 08 Nov 2018 21:47:12 +0000 en-US hourly 1 http://wordpress.org/?v=4.0 qPCR – Abalone Dg DNased RNA from yesterday and earlier today http://onsnetwork.org/kubu4/2009/06/25/qpcr-abalone-dg-dnased-rna-from-yesterday-and-earlier-today/ http://onsnetwork.org/kubu4/2009/06/25/qpcr-abalone-dg-dnased-rna-from-yesterday-and-earlier-today/#comments Thu, 25 Jun 2009 23:27:16 +0000 http://onsnetwork.org/kubu4/?p=993

Check Abalone RNA for residual gDNA contamination after DNase treatment. Ran qPCR with H.crach_16s_SYB_F/R primers. Plate layout/PCR set up here.

Results: Still gDNA in virtually every sample! This is totally insane. Will find/design primers that will only amplify gDNA to aid in subsequent analysis…

]]> http://onsnetwork.org/kubu4/2009/06/25/qpcr-abalone-dg-dnased-rna-from-yesterday-and-earlier-today/feed/ 0 qPCR – Re-DNased abalone Dg RNA from earlier today http://onsnetwork.org/kubu4/2009/06/08/qpcr-re-dnased-abalone-dg-rna-from-earlier-today-3/ http://onsnetwork.org/kubu4/2009/06/08/qpcr-re-dnased-abalone-dg-rna-from-earlier-today-3/#comments Tue, 09 Jun 2009 04:42:12 +0000 http://onsnetwork.org/kubu4/?p=1035

Done to verify removal of gDNA from RNA. Used H.crach_16s_syb_f/r primers. PCR workup/plate layout is here.

Results: Still f’ing gDNA! I’m pretty convinced that this is indeed due to the Ambion kit I’m using being old. Got mixed up with a newer kit, but neither had dates. Mac is going to be running a qPCR later today on DNased RNA that used the other Ambion kit. I will wait until the results of her qPCR to proceed.

]]> http://onsnetwork.org/kubu4/2009/06/08/qpcr-re-dnased-abalone-dg-rna-from-earlier-today-3/feed/ 0 qPCR – DNased abalone Dg RNA from earlier today http://onsnetwork.org/kubu4/2009/06/05/qpcr-dnased-abalone-dg-rna-from-earlier-today/ http://onsnetwork.org/kubu4/2009/06/05/qpcr-dnased-abalone-dg-rna-from-earlier-today/#comments Sat, 06 Jun 2009 04:47:58 +0000 http://onsnetwork.org/kubu4/?p=1041

Done to verify removal of gDNA from RNA. Used H.crach_16s_syb_f/r primers. PCR workup/plate layout is here.

Results: RNA is still contaminated. Will have to re-DNase the samples.

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